Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106978
Name   oriT_p54569CZ_2 in_silico
Organism   Enterobacter hormaechei strain 54569CZ
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP085759 (18460..18559 [+], 100 nt)
oriT length   100 nt
IRs (inverted repeats)      32..38, 42..48  (TTACGAT..ATCGTAA)
Location of nic site      61..62
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 100 nt

>oriT_p54569CZ_2
AATTAGAATAATTTGTTTTGTTTTCAAGCATTTACGATGAAATCGTAATTGCGTATGGTGTATAGCCATTAAGGGATACCATAACACGCCTTTTTTAAGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4608 GenBank   WP_021536814
Name   mobF_LJU41_RS22105_p54569CZ_2 insolico UniProt ID   _
Length   969 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 969 a.a.        Molecular weight: 108483.54 Da        Isoelectric Point: 9.7610

>WP_021536814.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLNITTITRQRVDKVVDYYADGADDYYAKDGNAMQWQGAGAEELGLTGEVEQKRFAKLLDGKVTDDISVM
RKTANSESKERLGYDLTFSAPKGVTLQALVNGDKRIIEAHDRAVTKAIEEAERLALGRFTVNKKTHVENT
NKLIVGKFRHETSRELDPDLHTHAFVMNLTKTSDGKWRSLTNDGIVNSLTLLGNVYKSELARELELAGYN
LRYDRNGTFDLAHFSQEQIAQFSQRSQQIEAELAKNGLSRETASHEQKNAAALKTRKSKGEVDRDVLYEG
WKNRAKELQIDFDSREWKGAANDDIARNTQPAALEKPIEYQADKVMTFAIRSLTERQSVITERELMDVAM
KHGYGRLTLDDIRAARERAITSGNLIKEEATYAAATTNKKQQNVAMTREQWVTELVKAGRNKDEARKLVD
KGITNGRLVQQKPRFTTQLAQQRERNILKMEREGRGKIQTPYTREFSEGWLASRTLKPEQLKAVMGIIHT
PNQFISVHGFAGTGKSYMTKSAADFLKEQGVHVTSLAPYGSQVKALQAEGLESRTLQSFLRASDKKIGPG
SVVFIDEAGVIPARQMEETMRVIRDAGARAVFLGDTKQTKAVEAGKPFEQLIKAGMETAYMKDIQRQKDP
ELLKAALNAAEGKIKASLTHVTSIVEEKNHDQRYRRIVGDYVAMPPTDRANALIITGTNDSRKKINEYIQ
AELGLKGQGINYPLLNRLDTTQAQRQHSKYYEKGAVIIPERDYSNGLKRGAVYTVLDTGPGNKLTVSDGS
GDTIAFSPARFSKLSVYSVEKTELAVGDQVRITRNDAHLDLANGDRFKVKGVQSGEVLLENEKGRLVKID
ANKPMYLGLAYASTVHSAQGLTCDKVFINMDTRSLTTAKDVYYVAVTRAKHEAVIYTDDEKKLDKAASRE
GFKTAALELEQLKRYAKEQQHDAREKGRDKTEPTQEKDNAKRKGKGHDKNNDERAFTSL

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(475-649)

UvrD-like helicase C-terminal domain (UvrD_C_2)

(850-896)

TrwC relaxase (TrwC)

(14-287)


  Protein structure



No available structure.




T4CP

ID   4701 GenBank   WP_021536815
Name   t4cp2_LJU41_RS22110_p54569CZ_2 insolico UniProt ID   _
Length   525 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 525 a.a.        Molecular weight: 58961.81 Da        Isoelectric Point: 9.3727

>WP_021536815.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MNEADKGKICLGALLFTPLVTWFIAAKALYGFTPKDARERLVLLIQQTFDLWPLWGALLVGEIIAIIGLI
IFFKFNHQIFKGAPFKKIYRGTRLVSQRALASITKEREPQITIADIPIPTKAEGTHISIAGATGVGKSTI
FAEMMKCCLMRGKMQRVASNKDRMIILDPDGDFLSRFYKKGDKILNPYDARTEGWVFFNEIKSDFDFERF
GVSIVPTAKDSQTEEWNGYGRLLFTEVSRKVFNTSRNPTMEEVFYWTNEVPLEELEEYVRGTKAQALFAG
SGRAVGSARFVLSNKLAAHLKMPAGNFSIREWLEDPKGGNLYITWTDEMREALKSLISCWTDSIFSIVLG
MSKSNSRKIWTFIDELESLDYLPSLRDALTKGRKKGLRVVTGYQSYSQVISIYGSDVAETLLSNHRTSVV
MAAGRLGERTLEFVSKSLGEIEGEREKTGISRRFGQLGTKNTNDDHKRERAVTPTEIATLDDLTGYIAFP
GNLPVAKFETHHVNYTRSNPVPGVVLRESTAFAGM

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(111-499)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 228..18018

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU41_RS22005 (LJU41_22005) 228..542 - 315 WP_023223516 hypothetical protein tfc15
LJU41_RS22010 (LJU41_22010) 545..862 - 318 WP_023223517 TrbM/KikA/MpfK family conjugal transfer protein -
LJU41_RS22015 (LJU41_22015) 867..1304 - 438 WP_023187074 hypothetical protein -
LJU41_RS22020 (LJU41_22020) 1316..1552 - 237 WP_021536804 H-NS histone family protein -
LJU41_RS22025 (LJU41_22025) 1615..2355 + 741 WP_042041934 lytic transglycosylase domain-containing protein virB1
LJU41_RS22030 (LJU41_22030) 2378..2695 + 318 WP_001267114 hypothetical protein -
LJU41_RS22035 (LJU41_22035) 2679..2969 + 291 WP_023223518 TrbC/VirB2 family protein virB2
LJU41_RS22040 (LJU41_22040) 3021..3335 + 315 WP_001053888 VirB3 family type IV secretion system protein virB3
LJU41_RS22045 (LJU41_22045) 3335..5965 + 2631 WP_042041932 VirB4 family type IV secretion/conjugal transfer ATPase virb4
LJU41_RS22050 (LJU41_22050) 5975..6682 + 708 WP_023187073 type IV secretion system protein virB5
LJU41_RS22055 (LJU41_22055) 6686..6922 + 237 WP_000733633 EexN family lipoprotein -
LJU41_RS22060 (LJU41_22060) 6941..7984 + 1044 WP_021536808 type IV secretion system protein virB6
LJU41_RS22065 (LJU41_22065) 8079..8249 + 171 WP_021536809 hypothetical protein -
LJU41_RS22070 (LJU41_22070) 8252..8968 + 717 WP_000914645 type IV secretion system protein virB8
LJU41_RS22075 (LJU41_22075) 8971..9858 + 888 WP_000744355 TrbG/VirB9 family P-type conjugative transfer protein virB9
LJU41_RS22080 (LJU41_22080) 9855..11009 + 1155 WP_001224296 type IV secretion system protein VirB10 virB10
LJU41_RS22085 (LJU41_22085) 11002..12033 + 1032 WP_042041926 P-type DNA transfer ATPase VirB11 virB11
LJU41_RS22090 (LJU41_22090) 11996..12550 + 555 WP_000616063 phospholipase D family protein -
LJU41_RS22095 (LJU41_22095) 12563..13045 + 483 WP_042041949 GNAT family protein -
LJU41_RS22100 (LJU41_22100) 13057..13251 + 195 WP_000529710 hypothetical protein -
LJU41_RS22105 (LJU41_22105) 13529..16438 - 2910 WP_021536814 MobF family relaxase -
LJU41_RS22110 (LJU41_22110) 16441..18018 - 1578 WP_021536815 type IV secretion system DNA-binding domain-containing protein virb4
LJU41_RS22115 (LJU41_22115) 18011..18358 - 348 WP_001139329 hypothetical protein -
LJU41_RS22120 (LJU41_22120) 18844..19278 + 435 WP_021536817 hypothetical protein -
LJU41_RS22125 (LJU41_22125) 19291..20001 + 711 WP_000686338 StbB family protein -
LJU41_RS22130 (LJU41_22130) 20003..20371 + 369 WP_015345231 plasmid stabilization protein StbC -
LJU41_RS22135 (LJU41_22135) 20585..21355 + 771 WP_021536818 SprT-like domain-containing protein -
LJU41_RS22140 (LJU41_22140) 21396..21584 - 189 WP_001001010 hypothetical protein -
LJU41_RS22145 (LJU41_22145) 21589..21846 - 258 WP_021536820 hypothetical protein -
LJU41_RS22150 (LJU41_22150) 21919..22074 - 156 WP_021536821 hypothetical protein -
LJU41_RS22155 (LJU41_22155) 22227..22469 - 243 WP_000526841 hypothetical protein -
LJU41_RS22160 (LJU41_22160) 22524..22811 - 288 WP_001098466 hypothetical protein -

Host bacterium

ID   7415 GenBank   NZ_CP085759
Plasmid name   p54569CZ_2 Incompatibility group   -
Plasmid size   34050 bp Coordinate of oriT [Strand]   18460..18559 [+]
Host baterium   Enterobacter hormaechei strain 54569CZ

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -