Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 106975 |
| Name | oriT_p48411CZ_1  |
| Organism | Enterobacter hormaechei strain 48411CZ |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP085781 (68540..68626 [-], 87 nt) |
| oriT length | 87 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 87 nt
>oriT_p48411CZ_1
GGGTGTCGGGGCGGAGCCCTGACCAGGTGGCATTTGTGATATCGTGCGTGCGCGGTATTACAAATGCACATCCTGTCCCGTCTTTCG
GGGTGTCGGGGCGGAGCCCTGACCAGGTGGCATTTGTGATATCGTGCGTGCGCGGTATTACAAATGCACATCCTGTCCCGTCTTTCG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 4606 | GenBank | WP_022650008 |
| Name | Relaxase_LJU35_RS23140_p48411CZ_1 |
UniProt ID | A0A4Q2QTK9 |
| Length | 899 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 899 a.a. Molecular weight: 102314.37 Da Isoelectric Point: 8.2756
>WP_022650008.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacterales]
MIPVIPPKRQDGKSSFGDLVSYVSVRDEPRDDDLMEAVRASGTKPEMPHSSRFSRLVDYATKLRDESFIS
LVDVMPDGGEWVNFYGVTCFHNCTSIESAAEEMEFSARKTKFAHNKSDPVFHYILSWQSHESPRPEQIYD
SVSHTLSRLGLAGHQFVAAVHTDTDNLHVHIAVNRVHPETGYLNRLAYSQEKLSKACRELELKHGFSPDN
GCYVIAPDNRIVRRTSVERDRQSAWKRGRNQSLREYIADTAIAGLRESPVTDWSSLHQRFAKEGLFLTKE
SGELKVKDGWDSERPGVTLSAFGHAFDTDKLIRKLGEFVPPVQDIFSQVPAVGRYEPDKIIMPSRPERMT
EKESLSDYAIARLRAPLIALDQDPTQRTIQSVHTLLAQSGLYLKEQHDRLVICDGYDSTRTPVRAERVWP
ALTKAILDSYKGGWQPVPKDIFQQVPPAEQFTGGSLEATPVSDREWRKLRTGSGPQGALKREIFSDKESL
WGYAVSHCRQDIESLIGSGEFTWERCHELLARQGLLLMREQQGLVVMDAYNHEQTPVKASHVHPDLTLAR
AEPHAGSFVSVPADIFERVKPASRYNPELAVSDRDIPGMKRDPELRRQRREARAAAREDLKARYAAWRAN
WQRPDLHGRERYQAIHDECRIRKARIRIEHRDPLVRKLHYHIAELQRMQALIALKETMKAERAELIGQGK
WYPPSYRQWVEAEAVKGDKAAISQLRGWDYRDRRGNAVKSTTDNRCVVICEPGGTPMYSGVPGLRASLKK
NGRVQFRDPETGRHICTDYGDRVIFRNSGDYDALKRDMVKVAPVLFSRSPSVAMAPEGNNEQFNTAFAKM
VAWHNVNHRDEGEYQISRPEVDQVRVESEMRFAEMTNQGSNTEAVRNESGPNWKPPSPL
MIPVIPPKRQDGKSSFGDLVSYVSVRDEPRDDDLMEAVRASGTKPEMPHSSRFSRLVDYATKLRDESFIS
LVDVMPDGGEWVNFYGVTCFHNCTSIESAAEEMEFSARKTKFAHNKSDPVFHYILSWQSHESPRPEQIYD
SVSHTLSRLGLAGHQFVAAVHTDTDNLHVHIAVNRVHPETGYLNRLAYSQEKLSKACRELELKHGFSPDN
GCYVIAPDNRIVRRTSVERDRQSAWKRGRNQSLREYIADTAIAGLRESPVTDWSSLHQRFAKEGLFLTKE
SGELKVKDGWDSERPGVTLSAFGHAFDTDKLIRKLGEFVPPVQDIFSQVPAVGRYEPDKIIMPSRPERMT
EKESLSDYAIARLRAPLIALDQDPTQRTIQSVHTLLAQSGLYLKEQHDRLVICDGYDSTRTPVRAERVWP
ALTKAILDSYKGGWQPVPKDIFQQVPPAEQFTGGSLEATPVSDREWRKLRTGSGPQGALKREIFSDKESL
WGYAVSHCRQDIESLIGSGEFTWERCHELLARQGLLLMREQQGLVVMDAYNHEQTPVKASHVHPDLTLAR
AEPHAGSFVSVPADIFERVKPASRYNPELAVSDRDIPGMKRDPELRRQRREARAAAREDLKARYAAWRAN
WQRPDLHGRERYQAIHDECRIRKARIRIEHRDPLVRKLHYHIAELQRMQALIALKETMKAERAELIGQGK
WYPPSYRQWVEAEAVKGDKAAISQLRGWDYRDRRGNAVKSTTDNRCVVICEPGGTPMYSGVPGLRASLKK
NGRVQFRDPETGRHICTDYGDRVIFRNSGDYDALKRDMVKVAPVLFSRSPSVAMAPEGNNEQFNTAFAKM
VAWHNVNHRDEGEYQISRPEVDQVRVESEMRFAEMTNQGSNTEAVRNESGPNWKPPSPL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4Q2QTK9 |
T4CP
| ID | 4699 | GenBank | WP_022650006 |
| Name | trbC_LJU35_RS23155_p48411CZ_1 |
UniProt ID | _ |
| Length | 766 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 766 a.a. Molecular weight: 87660.94 Da Isoelectric Point: 6.8660
>WP_022650006.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MNYTPVNQALMKRNAWNSPVLELLRDHLLYAGIMAGSVVAGFIWPLAIPLLLLLAIVASISFGTHRWRMP
MRMPVHLNKVDPSEDRKVRRSLFRRFPSLFQYETTQESKGKGIFYLGYRRMNDIGRELWLTIDDLTRHVM
FFASTGGGKTETIYAWMLNSFCWGRGFTFVDGKAQNDTARTCYYLARRFGREDDVEYINFMNSGMSRSEM
IHNGDKSRPQSHQWNPFCYSTEAFVAETMQSMLPTNVQGGEWQSRAIAMNKALVFGTKFYCVRENKTMSL
QLLREFMPLEKLAELYCRAIDDQWPEEAVSPLYNYLVDVPGFDMATVRQPSAWTEEPRKQHSYLTGQFLE
TFNTFTETFGNIFAEDAGDIDIRDSIHSDRILLVLIPAMNTSQHTTSALGRMFVTQQSMILARDLGHRLE
GLDSEALEITKYKGPFPYMNYLDEVGAYYTERIAVQATQVRSLDFSIVMMSQDQERIESQTSATNVATLM
QNAGTKVAGKIVSDDKTAKTITNAAGQEARANMVSLQRRDGIIGTSWIDGDHINIQMENKINVQDLIKLQ
PGENYTVFQGDPVPGASFYIEPQDKTCNAPVIINRYITINPPNLKQLRKLVPRTAQRRLPAPENVSKIIG
VLTEKPSRRRKAAKAEPWKIIDTFQQRLANRQEAHNLMTEYDTDHRGREENLWEEALEVIRTTTMAERTI
RYITLNKPESEPTQVSAEEIQLAPDAILRTLTLPQPAYIPPSRYRNEPFISPKLPDSPHPDMEWPE
MNYTPVNQALMKRNAWNSPVLELLRDHLLYAGIMAGSVVAGFIWPLAIPLLLLLAIVASISFGTHRWRMP
MRMPVHLNKVDPSEDRKVRRSLFRRFPSLFQYETTQESKGKGIFYLGYRRMNDIGRELWLTIDDLTRHVM
FFASTGGGKTETIYAWMLNSFCWGRGFTFVDGKAQNDTARTCYYLARRFGREDDVEYINFMNSGMSRSEM
IHNGDKSRPQSHQWNPFCYSTEAFVAETMQSMLPTNVQGGEWQSRAIAMNKALVFGTKFYCVRENKTMSL
QLLREFMPLEKLAELYCRAIDDQWPEEAVSPLYNYLVDVPGFDMATVRQPSAWTEEPRKQHSYLTGQFLE
TFNTFTETFGNIFAEDAGDIDIRDSIHSDRILLVLIPAMNTSQHTTSALGRMFVTQQSMILARDLGHRLE
GLDSEALEITKYKGPFPYMNYLDEVGAYYTERIAVQATQVRSLDFSIVMMSQDQERIESQTSATNVATLM
QNAGTKVAGKIVSDDKTAKTITNAAGQEARANMVSLQRRDGIIGTSWIDGDHINIQMENKINVQDLIKLQ
PGENYTVFQGDPVPGASFYIEPQDKTCNAPVIINRYITINPPNLKQLRKLVPRTAQRRLPAPENVSKIIG
VLTEKPSRRRKAAKAEPWKIIDTFQQRLANRQEAHNLMTEYDTDHRGREENLWEEALEVIRTTTMAERTI
RYITLNKPESEPTQVSAEEIQLAPDAILRTLTLPQPAYIPPSRYRNEPFISPKLPDSPHPDMEWPE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 74749..105839
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LJU35_RS23145 (LJU35_23145) | 71765..71968 | - | 204 | WP_123839396 | hypothetical protein | - |
| LJU35_RS23150 (LJU35_23150) | 72102..72371 | - | 270 | WP_022650007 | hypothetical protein | - |
| LJU35_RS23155 (LJU35_23155) | 72445..74745 | - | 2301 | WP_022650006 | F-type conjugative transfer protein TrbC | - |
| LJU35_RS23160 (LJU35_23160) | 74749..76005 | - | 1257 | WP_126530067 | hypothetical protein | trbB |
| LJU35_RS23165 (LJU35_23165) | 75986..77194 | - | 1209 | WP_022650004 | hypothetical protein | trbA |
| LJU35_RS23170 (LJU35_23170) | 77515..78147 | - | 633 | WP_022650003 | plasmid IncI1-type surface exclusion protein ExcA | - |
| LJU35_RS23175 (LJU35_23175) | 78209..80368 | - | 2160 | WP_022650002 | DotA/TraY family protein | traY |
| LJU35_RS23180 (LJU35_23180) | 80426..80968 | - | 543 | WP_022650001 | conjugal transfer protein TraX | - |
| LJU35_RS23185 (LJU35_23185) | 80965..82167 | - | 1203 | WP_022650000 | conjugal transfer protein TraW | traW |
| LJU35_RS23190 (LJU35_23190) | 82180..82749 | - | 570 | WP_022649999 | hypothetical protein | traV |
| LJU35_RS23195 (LJU35_23195) | 82749..85802 | - | 3054 | WP_022649998 | hypothetical protein | traU |
| LJU35_RS23200 (LJU35_23200) | 85889..86614 | - | 726 | WP_063864836 | hypothetical protein | traT |
| LJU35_RS23205 (LJU35_23205) | 86798..87202 | - | 405 | WP_022649996 | DUF6750 family protein | traR |
| LJU35_RS23210 (LJU35_23210) | 87237..87761 | - | 525 | WP_022649995 | conjugal transfer protein TraQ | traQ |
| LJU35_RS23215 (LJU35_23215) | 87761..88534 | - | 774 | WP_123839398 | conjugal transfer protein TraP | traP |
| LJU35_RS23220 (LJU35_23220) | 88515..89822 | - | 1308 | WP_022649993 | conjugal transfer protein TraO | traO |
| LJU35_RS23225 (LJU35_23225) | 89822..90802 | - | 981 | WP_022649992 | DotH/IcmK family type IV secretion protein | traN |
| LJU35_RS23230 (LJU35_23230) | 90813..91430 | - | 618 | WP_179297403 | DotI/IcmL family type IV secretion protein | traM |
| LJU35_RS23235 (LJU35_23235) | 91520..91870 | - | 351 | WP_022649990 | hypothetical protein | traL |
| LJU35_RS23240 (LJU35_23240) | 91888..95430 | - | 3543 | WP_022649989 | LPD7 domain-containing protein | - |
| LJU35_RS23245 (LJU35_23245) | 95502..95789 | - | 288 | WP_022649988 | hypothetical protein | traK |
| LJU35_RS23250 (LJU35_23250) | 95786..96946 | - | 1161 | WP_022649987 | plasmid transfer ATPase TraJ | virB11 |
| LJU35_RS23255 (LJU35_23255) | 96959..97765 | - | 807 | WP_022649986 | type IV secretory system conjugative DNA transfer family protein | traI |
| LJU35_RS23260 (LJU35_23260) | 97762..98235 | - | 474 | WP_022649985 | DotD/TraH family lipoprotein | - |
| LJU35_RS23265 (LJU35_23265) | 98306..98722 | - | 417 | WP_123839394 | hypothetical protein | - |
| LJU35_RS23270 (LJU35_23270) | 98831..100036 | - | 1206 | WP_022649984 | conjugal transfer protein TraF | - |
| LJU35_RS23275 (LJU35_23275) | 100142..100978 | - | 837 | WP_022649983 | hypothetical protein | traE |
| LJU35_RS23280 (LJU35_23280) | 101125..101601 | - | 477 | WP_022649982 | hypothetical protein | - |
| LJU35_RS23285 (LJU35_23285) | 101621..102781 | - | 1161 | WP_022649981 | site-specific integrase | - |
| LJU35_RS23290 (LJU35_23290) | 103673..104701 | - | 1029 | WP_342453751 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| LJU35_RS23295 (LJU35_23295) | 104698..105372 | - | 675 | WP_022649979 | prepilin peptidase | - |
| LJU35_RS23300 (LJU35_23300) | 105357..105839 | - | 483 | WP_045261878 | lytic transglycosylase domain-containing protein | virB1 |
| LJU35_RS23305 (LJU35_23305) | 105924..106538 | - | 615 | WP_022649977 | type 4 pilus major pilin | - |
| LJU35_RS23310 (LJU35_23310) | 106552..107646 | - | 1095 | WP_032623575 | type II secretion system F family protein | - |
Host bacterium
| ID | 7412 | GenBank | NZ_CP085781 |
| Plasmid name | p48411CZ_1 | Incompatibility group | - |
| Plasmid size | 107928 bp | Coordinate of oriT [Strand] | 68540..68626 [-] |
| Host baterium | Enterobacter hormaechei strain 48411CZ |
Cargo genes
| Drug resistance gene | blaLAP-2, qnrS1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIC1, AcrIIA7 |