Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 106972
• Name: oriT_pL8-A
• Organism: Enterococcus faecalis strain L8
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP042217 (2859..2896 [+], 38 nt)
• oriT length: 38 nt
• IRs (inverted repeats): 14..22, 29..37 (TAAAGTATA..TATACTTTA) 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 38 nt

>oriT_pL8-A
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_FOA09_RS13430_pL8-A

• ID: 4603
• GenBank: WP_000119405
• Name: mobV_FOA09_RS13430_pL8-A
• UniProt ID: F5X340
• Length: 420 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 420 a.a.
• Molecular weight: 49660.33 Da
• Isoelectric Point: 9.9693

>WP_000119405.1 MULTISPECIES: MobV family relaxase [Bacteria]
MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTR
KTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVK
KDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDN
DRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSL
IYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

• AlphaFold DB: F5X340

Host bacterium

• ID: 7409
• GenBank: NZ_CP042217
• Plasmid name: pL8-A
• Incompatibility group: -
• Plasmid size: 85951 bp
• Coordinate of oriT [Strand]: 2859..2896 [+]
• Host baterium: Enterococcus faecalis strain L8

Cargo genes

• Drug resistance gene: tet(L)
• Virulence gene: -
• Metal resistance gene: tcrB
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21