Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 106965
• Name: oriT_pRetMIM1b
• Organism: Rhizobium etli bv. mimosae str. Mim1
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NC_021907 (84397..84425 [+], 29 nt)
• oriT length: 29 nt
• IRs (inverted repeats): 14..19, 24..29 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 29 nt

>oriT_pRetMIM1b
AGGGCGCAATATACGTCGCTGGCGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_REMIM1_RS22245_pRetMIM1b

• ID: 4600
• GenBank: WP_041678954
• Name: traA_REMIM1_RS22245_pRetMIM1b
• UniProt ID: _
• Length: 1545 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1545 a.a.
• Molecular weight: 171061.23 Da
• Isoelectric Point: 7.5193

>WP_041678954.1 Ti-type conjugative transfer relaxase TraA [Rhizobium etli]
MAIMFVRAQVISRGAGRSIVSAAAYRHRARMMDEQAGTSFSYRGGSRELVHEELALPDQIPAWLTAAIGG
QSVARASEVLWNALDASETRADAQLARELIIALPEELTRAENIALAREFVRDSFTSMGMVADWVYHDNDG
NPHIHLMTTLRPLTEEGFGPKKVPVTGEDGKPLRVVTPDRPNGKIVYRVWAGDKETLKAWKIAWAETANR
HLALAGHEIRLDGRSYAEQGLDGIAQRHLGPEKAALARKRRELYFAPADLARRHEMADRLLAEPELLLKQ
LGNERSTFDEKDIAKALHRYVDDPADFANIRARLMASDELVMLKPQEFDGETLKVADPAVFTTRGMLRIE
YDMAQSVRVLSERAGFGVSARQVDAAIERVESGDPKHPFRLDAEQVDAIRHVTGDSGIAAVVGLAGAGKS
TLLAAARLAWEGEGRRVIGAAVAGKAAEGLEDSSGIKSRTLAAWEMAWANGRDTLHRGDVLVIDEAGMVS
SQQMARVLDIVEAAGAKVVLVGDAMQLQPIQAGAAFRAISDRIGFAELAGVRRQHEEWAREASRLFARGE
VEKGLDAYARHGRLVEAETRQEIVDRIVADWSQARREAIGRSVSEGRDGNLRGDELLVLAHTNDDVGKLN
EALRAVMAGEGALSDSRTFQTARGAREFAVGDRIIFLENARFLEPRARSSGPQYVKNGMLGTVISTGDKR
GEALMSVRLDNGHKVVFSEDSYRNVDHGYAATIHKAQGATVERTLVLATGMMDQHLTYVAMTRHRDRVDL
YAASEDFEPKPEWGRKPRVDHAAGVTGELVETGEAKFRPDDEDADDTPYADIRTDDGTVHRLWGVSLPKA
LEEGGLSEGDTVTLRKDGVERVKVQIAVVDEETGQKRYEEREVDRNVWTARQIETAEARQDRIERESHRP
DLFKQLVERLSRSGAKTTTLDFESEAGYRAHAQDFARRRGFEYLSLAAAEIEGDIARRFAWIAAKREQVE
KLWERASMALGFAIERERRVSYSEVRTAPHDVPGANAGKARYLIPPTTDFARSLNEDARLAQLSSPAWKD
REAILRPVLEKIYRDPDRALVALNARASEAGTEPRRLADDLAASPDRLGRLRGSELIVDGRAAREERNVA
LAAVTELLPLARAHASEFRRNAKRFEIREQARRAHMSLSVPALSEGAMARLMEIEAVRNRGGDDAYKTAF
ALAAQDRTVVREIKAVSEAITARFGWSAFTAKADAIADRNMVDRMPEDLTDERREKLIRLVEAVRRFAEE
QHLAERQDRSKIVAAASAVAGTELEKWKVTMPPMFAAVTEFRTPVDEEARSRVLSAPLYRQQRAALADAA
RTVWRDPAGAVGKIEELFAKGFAADRTAAAVTNDPAAYGALRGSGRLIDRMLASGRERKEAVQAVPEAAT
RLRSLGTAYANLFDAERQGIIDERRRMAVAIPGLSKVAEEALMRLTAEARKNDRKRGIATAPLDANIRRE
FADVSRALDQRFGRNAIIRGDKDLINRVPEAQRQAFVAMQEKLKVLQQAVRAQSSQQVLSERRQRGVNRG
RGIDI

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (17-249)

• AAA domain (AAA_30) (391-578)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1312-1406)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1049-1127)

Protein structure

No available structure.

Host bacterium

• ID: 7402
• GenBank: NC_021907
• Plasmid name: pRetMIM1b
• Incompatibility group: -
• Plasmid size: 252689 bp
• Coordinate of oriT [Strand]: 84397..84425 [+]
• Host baterium: Rhizobium etli bv. mimosae str. Mim1

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -