Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106961
Name   oriT_pSI173-1 in_silico
Organism   Salmonella enterica subsp. enterica serovar Indiana strain SI173
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP050758 (33576..33664 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pSI173-1
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4599 GenBank   WP_071447698
Name   nikB_HEC84_RS22890_pSI173-1 insolico UniProt ID   A0A1S7B047
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103868.35 Da        Isoelectric Point: 7.4198

>WP_071447698.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMEGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A1S7B047


Auxiliary protein

ID   2068 GenBank   WP_001283947
Name   WP_001283947_pSI173-1 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   4694 GenBank   WP_001289275
Name   trbC_HEC84_RS22895_pSI173-1 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86957.15 Da        Isoelectric Point: 6.7713

>WP_001289275.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHLTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 39042..78460

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HEC84_RS22895 (HEC84_22895) 36758..39049 - 2292 WP_001289275 F-type conjugative transfer protein TrbC -
HEC84_RS22900 (HEC84_22900) 39042..40112 - 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
HEC84_RS22905 (HEC84_22905) 40131..41339 - 1209 WP_001383960 IncI1-type conjugal transfer protein TrbA trbA
HEC84_RS22910 (HEC84_22910) 41557..42543 - 987 WP_001257838 hypothetical protein -
HEC84_RS22915 (HEC84_22915) 42816..42968 + 153 WP_001331364 Hok/Gef family protein -
HEC84_RS22920 (HEC84_22920) 43040..43291 - 252 WP_001291964 hypothetical protein -
HEC84_RS23725 43790..43885 + 96 WP_001303310 DinQ-like type I toxin DqlB -
HEC84_RS22925 (HEC84_22925) 43950..44126 - 177 WP_001054900 hypothetical protein -
HEC84_RS22930 (HEC84_22930) 44458..44667 + 210 WP_000062602 HEAT repeat domain-containing protein -
HEC84_RS22935 (HEC84_22935) 44739..45401 - 663 WP_000644792 plasmid IncI1-type surface exclusion protein ExcA -
HEC84_RS22940 (HEC84_22940) 45466..47634 - 2169 WP_000698366 DotA/TraY family protein traY
HEC84_RS22945 (HEC84_22945) 47731..48315 - 585 WP_001037996 IncI1-type conjugal transfer protein TraX -
HEC84_RS22950 (HEC84_22950) 48344..49546 - 1203 WP_001189156 IncI1-type conjugal transfer protein TraW traW
HEC84_RS22955 (HEC84_22955) 49513..50127 - 615 WP_000337399 IncI1-type conjugal transfer protein TraV traV
HEC84_RS22960 (HEC84_22960) 50127..53171 - 3045 WP_001024776 IncI1-type conjugal transfer protein TraU traU
HEC84_RS22965 (HEC84_22965) 53261..54061 - 801 WP_001164792 IncI1-type conjugal transfer protein TraT traT
HEC84_RS22970 (HEC84_22970) 54045..54233 - 189 WP_001277255 putative conjugal transfer protein TraS -
HEC84_RS22975 (HEC84_22975) 54297..54701 - 405 WP_000086960 IncI1-type conjugal transfer protein TraR traR
HEC84_RS22980 (HEC84_22980) 54752..55279 - 528 WP_001055569 conjugal transfer protein TraQ traQ
HEC84_RS22985 (HEC84_22985) 55279..55983 - 705 WP_023155244 IncI1-type conjugal transfer protein TraP traP
HEC84_RS22990 (HEC84_22990) 55983..57272 - 1290 WP_001271994 conjugal transfer protein TraO traO
HEC84_RS22995 (HEC84_22995) 57275..58258 - 984 WP_021513963 IncI1-type conjugal transfer protein TraN traN
HEC84_RS23000 (HEC84_23000) 58269..58961 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
HEC84_RS23005 (HEC84_23005) 58958..59305 - 348 WP_001055900 conjugal transfer protein traL
HEC84_RS23010 (HEC84_23010) 59323..63090 - 3768 WP_077134477 LPD7 domain-containing protein -
HEC84_RS23015 (HEC84_23015) 63180..63731 - 552 WP_000014584 phospholipase D family protein -
HEC84_RS23020 (HEC84_23020) 63746..64036 - 291 WP_001299214 hypothetical protein traK
HEC84_RS23025 (HEC84_23025) 64033..65181 - 1149 WP_001024976 plasmid transfer ATPase TraJ virB11
HEC84_RS23030 (HEC84_23030) 65178..65996 - 819 WP_000646097 IncI1-type conjugal transfer lipoprotein TraI traI
HEC84_RS23035 (HEC84_23035) 65993..66451 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
HEC84_RS23040 (HEC84_23040) 66846..67430 - 585 WP_000977520 histidine phosphatase family protein -
HEC84_RS23045 (HEC84_23045) 67490..68692 - 1203 WP_000976357 conjugal transfer protein TraF -
HEC84_RS23050 (HEC84_23050) 68778..69602 - 825 WP_015058912 conjugal transfer protein TraE traE
HEC84_RS23055 (HEC84_23055) 69753..70907 - 1155 WP_001139955 site-specific integrase -
HEC84_RS23775 72322..72546 + 225 Protein_82 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
HEC84_RS23060 (HEC84_23060) 72543..73979 - 1437 WP_015058913 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
HEC84_RS23065 (HEC84_23065) 73967..74623 - 657 WP_001193551 A24 family peptidase -
HEC84_RS23070 (HEC84_23070) 74608..75168 - 561 WP_000014005 lytic transglycosylase domain-containing protein virB1
HEC84_RS23075 (HEC84_23075) 75178..75792 - 615 WP_000908228 type 4 pilus major pilin -
HEC84_RS23080 (HEC84_23080) 75809..76894 - 1086 WP_001208803 type II secretion system F family protein -
HEC84_RS23085 (HEC84_23085) 76907..78460 - 1554 WP_000362204 ATPase, T2SS/T4P/T4SS family virB11
HEC84_RS23090 (HEC84_23090) 78471..78923 - 453 WP_001247333 type IV pilus biogenesis protein PilP -
HEC84_RS23095 (HEC84_23095) 78910..80205 - 1296 WP_000752776 type 4b pilus protein PilO2 -
HEC84_RS23100 (HEC84_23100) 80198..81880 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
HEC84_RS23105 (HEC84_23105) 81894..82331 - 438 WP_000539811 type IV pilus biogenesis protein PilM -
HEC84_RS23110 (HEC84_23110) 82331..83398 - 1068 WP_021513968 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   7398 GenBank   NZ_CP050758
Plasmid name   pSI173-1 Incompatibility group   IncI1
Plasmid size   87883 bp Coordinate of oriT [Strand]   33576..33664 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Indiana strain SI173

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -