Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 106948
• Name: oriT_psymB
• Organism: Sinorhizobium meliloti strain M270
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP021814 (918092..918120 [-], 29 nt)
• oriT length: 29 nt
• IRs (inverted repeats): 14..19, 24..29 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 29 nt

>oriT_psymB
AGGGCGCAATATACGTCGCTGGCGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_CDO26_RS30085_psymB

• ID: 4588
• GenBank: WP_088198608
• Name: traA_CDO26_RS30085_psymB
• UniProt ID: _
• Length: 1539 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1539 a.a.
• Molecular weight: 169908.58 Da
• Isoelectric Point: 9.5024

>WP_088198608.1 Ti-type conjugative transfer relaxase TraA [Sinorhizobium meliloti]
MAIMFVRAQVIGRGAGRSIVSAAAYRHRTRMIDEQAGTSFSYRGGASELVHEELALPDDIPAWLKAAIDG
QSVAKASEALWNAVEAHETRADAQLARELIIALPEELTRAENIALVREFVRDNLTSKGMVADWVYHDKDG
NPHIHLMAALRPLTEQGFGPKKVPVLGEDGEPLRVVTPDRPNGKIVYKLWAGDKETIKAWKIAWAETANR
HLALAGHEIRLDGRSYAEQGLDGIAQKHLGPEKAALARKGIAMYFAPADFARRQEMADRLLAEPGLLLKQ
LGNERSTFDERDIARALHRYVDDPVDFANIRARLMASDELVLLKPQQVDAETGKAKQPAVFTTREMLRIE
YAMAQSAEVLSRRKGFGVSNARAAAAVRSIETADTEKPFRLDPEQVDAVRHVTRDNAIAAVVGLAGAGKS
TLLAAARVAWEGEGRRVIGAALAGKAAEGLEDSSGIRSRTLASWELAWESGREQLQRGDVLVIDEAGMVS
SQQMARILKAVEDAGAKAVLVGDALQLQPIEAGAAFRAITERIGFAELAGVRRQRDAWARDASRLFARSK
VEEGLDAYAQQGRIVETETRAEIVDRIVADWANARRDLLQKSADGEHPGRLRGDELLVLAHTNDDVRKLN
EALRQVMIGEGALAGAREFQTARGLREFAAGDRIIFLENARFVEPRARRLGPQYVKNGMLGTVVSTGDRR
GDTLLSVRLDSGRDVVISQDSYRNVDHGYAATIHKSQGSTVDRTFVLATGMMDQHLTYVAMTRHRDRADL
YAAKEDFEAKPDWGRRPRVDHAAGVTGELVKEGMAKFRPNDEDADESPYADIRTDDGTVQRLWGVSLPKA
LKDAGVAEGDTITLRKDGVERVKVQVPIVDAQTGEKRFEERQVDRNVWSASQLETAAARRERIERESHRP
QLFKQLVERLSRSGAKTTTLDFEDEAGYQAQARDFARRRGLYHLSLVAAGMEAEVLRRWAGIAEKREQVA
KLWERASVALGFAIERERRVAYNEERTETLSTGIPSDGKYLVPPTTTFSRSVAEDARLAQLSSQRWKERE
AIVHPVLAKIYRDPDGALSALNALASDAAIEPRKLAEDLGLAPDRLGRLRGSELVVDGRAARDERTAATV
ALSELLPLARAHATEFRRNAERFGIREQQRRAHMALSVPALSKTAMARLVEIEAVREQGGDDAYRTAFAF
AVEDRLLVQEVKAVNEALTARFGWSAFTAKADVIAERNIAERMPEDLAPERREKLTRLFAVIRRFAEEQH
LAERQDRSKIVAGASVELGKGTFAVLPMLAPVTEFKTTVDEEARERALAAPHYAHHRAALVETATRVWRD
PADAIGKIEDLIVKGFAGERIAAAVTNDPAAYGALRGSDRIMDKLLAVGRERKGALQAVPEAASRIRSLG
ASYASALDAETRSITEERRRMAVAIPGLSPAAEDALKRLAAQIKNKDGKLDVAAGSLDPHIAREFAKVSR
ALDERFGRNAILRGETDVINRVSPAQRRAFEAMRDRLQVLQQAVRIQSSQKIVSERRQRAINQSRGIRM

Protein domains

Predicted by InterproScan.

• AAA domain (AAA_30) (391-578)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1309-1400)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1047-1125)

• MobA/MobL family (MobA_MobL) (17-249)

Protein structure

No available structure.

T4CP

tcpA_CDO26_RS37485_psymB

• ID: 4679
• GenBank: WP_094183236
• Name: tcpA_CDO26_RS37485_psymB
• UniProt ID: _
• Length: 947 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 947 a.a.
• Molecular weight: 101760.56 Da
• Isoelectric Point: 4.6250

>WP_094183236.1 FtsK/SpoIIIE domain-containing protein [Sinorhizobium meliloti]
MRIPRTNFSPAALYDANHADDYEDPHPAAQGAAHVPAAPEAAAHDHLFEAPEAPRRAPGHRDEETGYAGR
RAARLYGHGSAYAPAEVTVSTRDPLPTLAEITGLSGEPGWESHFFLSPNVRFTRTPERELMKRHPPAPEE
SRIEADEAAAEASDAETVMDVVPAEPAPSVVETELPSYSPSELLRVLTQQLPSWSAARSQAPEASVTKPA
ITESVAVAEEGPATSETTALPVTDEVPVVPHALPVANLAPESAAVEEDVPHQADARLAYLSDFAFFEFMP
LEVAAVPPTVTEPVKEAARIPAPIAAAPKVSPPKIVAAMPVEIRPQPPTAISSLFRVVECRRPEPATAEP
VTAAPQDIAAEPAAAEAAALPAHQFVETPAPALAEPAIVPASEPAPQAPVTRAAITMPAVIQRSSPSLPP
IGAIEPLQGGDAYEFPSKELLQEPPQGQGFFMTQEQLEQNAGLLESVLEDFGVKGEIIHVRPGPVVTLYE
FEPAPGVKSSRVIGLADDIARSMSALSARVAVVPGRNVIGIELPNATRETVYFRELIESGDFQKTGCKLA
LCLGKTIGGEPVIAELAKMPHLLVAGTTGSGKSVAINTMILSLLYRLKPEECRLIMVDPKMLELSVYDGI
PHLLTPVVTDPKKAVMALKWAVREMEDRYRKMSRLGVRNIDGYNQRAAAAREKGAPILATVQTGFEKGTG
EPLFEQQEMDLSPMPYIVVIVDEMADLMMVAGKEIEGAIQRLAQMARAAGIHLIMATQRPSVDVITGTIK
ANFPTRISFQVTSKIDSRTILGEQGAEQLLGQGDMLHMAGGGRIARVHGPFVSDQEVEHVVAHLKTQGRP
EYLETVTADEEEEEVEEDQGAVFDKSAIAAEDGNELYDQAVKVVLRDKKCSTSYIQRRLGIGYNRAASLV
ERMEKDGLVGPANHVGKREIIYGNRDNAPKPESDDLD

Protein domains

Predicted by InterproScan.

• FtsK alpha domain (FtsK_alpha) (435-535)

• Ftsk gamma domain (FtsK_gamma) (872-931)

• FtsK/SpoIIIE family (FtsK_SpoIIIE) (544-762)

Protein structure

No available structure.

Host bacterium

• ID: 7385
• GenBank: NZ_CP021814
• Plasmid name: psymB
• Incompatibility group: -
• Plasmid size: 1694441 bp
• Coordinate of oriT [Strand]: 918092..918120 [-]
• Host baterium: Sinorhizobium meliloti strain M270

Cargo genes

• Drug resistance gene: -
• Virulence gene: htpB
• Metal resistance gene: actP
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA7