Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 106928 |
| Name | oriT_pN12-0931-a  |
| Organism | Salmonella enterica subsp. enterica serovar Kentucky strain N12-0931 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP092010 (40138..40243 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_pN12-0931-a
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 4579 | GenBank | WP_004187323 |
| Name | Relaxase_MA811_RS23785_pN12-0931-a |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 4667 | GenBank | WP_004206885 |
| Name | t4cp2_MA811_RS23490_pN12-0931-a |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 43039..62238
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MA811_RS23765 (MA811_23760) | 38123..38434 | + | 312 | WP_004187333 | hypothetical protein | - |
| MA811_RS23770 (MA811_23765) | 38567..39103 | + | 537 | WP_004187332 | hypothetical protein | - |
| MA811_RS23775 (MA811_23770) | 39605..40000 | - | 396 | WP_019725163 | hypothetical protein | - |
| MA811_RS23780 (MA811_23775) | 40035..40562 | + | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| MA811_RS23785 (MA811_23780) | 40549..42528 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| MA811_RS23790 (MA811_23785) | 42542..43042 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| MA811_RS23795 (MA811_23790) | 43039..43818 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| MA811_RS23800 (MA811_23795) | 43829..44992 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| MA811_RS23805 (MA811_23800) | 44982..45242 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| MA811_RS23810 (MA811_23805) | 45267..48614 | + | 3348 | WP_223807062 | LPD7 domain-containing protein | - |
| MA811_RS23815 (MA811_23810) | 48580..49092 | + | 513 | WP_011091071 | hypothetical protein | traL |
| MA811_RS23820 (MA811_23815) | 49093..49305 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| MA811_RS23825 (MA811_23820) | 49277..49687 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| MA811_RS23830 (MA811_23825) | 49755..50468 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| MA811_RS23835 (MA811_23830) | 50477..51628 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| MA811_RS23840 (MA811_23835) | 51640..52989 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| MA811_RS23845 (MA811_23840) | 53001..53705 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| MA811_RS23850 (MA811_23845) | 53729..54259 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| MA811_RS23855 (MA811_23850) | 54276..54665 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| MA811_RS23860 (MA811_23855) | 54711..55205 | + | 495 | WP_004187480 | hypothetical protein | - |
| MA811_RS23865 (MA811_23860) | 55202..58252 | + | 3051 | WP_213758542 | conjugal transfer protein | traU |
| MA811_RS23870 (MA811_23865) | 58249..59457 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| MA811_RS23875 (MA811_23870) | 59454..60050 | + | 597 | WP_032419542 | hypothetical protein | - |
| MA811_RS23880 (MA811_23875) | 60043..62238 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| MA811_RS23885 (MA811_23880) | 62240..62893 | + | 654 | WP_015060005 | hypothetical protein | - |
| MA811_RS23890 (MA811_23885) | 62974..63204 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 7365 | GenBank | NZ_CP092010 |
| Plasmid name | pN12-0931-a | Incompatibility group | IncL/M |
| Plasmid size | 63499 bp | Coordinate of oriT [Strand] | 40138..40243 [+] |
| Host baterium | Salmonella enterica subsp. enterica serovar Kentucky strain N12-0931 |
Cargo genes
| Drug resistance gene | blaOXA-48 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |