Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 106923 |
| Name | oriT_pSA20080453.1  |
| Organism | Salmonella enterica strain SA20080453 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP030195 (34200..34615 [+], 416 nt) |
| oriT length | 416 nt |
| IRs (inverted repeats) | 320..325, 333..338 (GCAGTG..CACTGC) 197..204, 206..213 (TTCAATTC..GAATTGAA) 85..90, 96..101 (TGCTTT..AAAGCA) 81..88, 93..100 (GCTTTGCT..AGCAAAGC) |
| Location of nic site | 315..316 |
| Conserved sequence flanking the nic site |
TCCTGCATCG |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 416 nt
>oriT_pSA20080453.1
TTCTTTGTTTCCCTGTCAAATACATTGTGGTTTTGATAAAATCATCATTATCTTTAAACACATTAAAATCTTCATTTTTAGCTTTGCTTTTCAGCAAAGCACTGGCAAGCAACTTCATGCCTTTAGCAACAGTTAAACCGGGTTCATCTGGATAAAGAGTTTTAAGACAATCTAATAGATCATCATGTTGGCTTTCTTCAATTCTGAATTGAACTTTTTTCATAAATCTCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAACGTTGCGGAATGGCGTCAAGGACGTCATTTTGCGGCGTTTGCCCTATCCTGCATCGCAGTGGATCTGGCACTGCTCATGAATTGTGCTTCAGCATCAACAAAAATGCAAAAAGCTGAAGTTGTCATAGCTGAAGCTCCGTTCGTCTTCGACC
TTCTTTGTTTCCCTGTCAAATACATTGTGGTTTTGATAAAATCATCATTATCTTTAAACACATTAAAATCTTCATTTTTAGCTTTGCTTTTCAGCAAAGCACTGGCAAGCAACTTCATGCCTTTAGCAACAGTTAAACCGGGTTCATCTGGATAAAGAGTTTTAAGACAATCTAATAGATCATCATGTTGGCTTTCTTCAATTCTGAATTGAACTTTTTTCATAAATCTCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAACGTTGCGGAATGGCGTCAAGGACGTCATTTTGCGGCGTTTGCCCTATCCTGCATCGCAGTGGATCTGGCACTGCTCATGAATTGTGCTTCAGCATCAACAAAAATGCAAAAAGCTGAAGTTGTCATAGCTGAAGCTCCGTTCGTCTTCGACC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 4577 | GenBank | WP_071678439 |
| Name | mobP1_CHD54_RS22990_pSA20080453.1 |
UniProt ID | _ |
| Length | 388 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 388 a.a. Molecular weight: 44607.14 Da Isoelectric Point: 10.5353
>WP_071678439.1 MobP1 family relaxase [Salmonella enterica]
MGVWVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAITRQGVRNSI
DYMSRESGLPVMSESGQVWKGDEIQEAKEHMIDRANDPQYVMNDKGKENKKITQNIVFSPPVSARVKPED
LLESVRKTMHKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKRDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDVGYDHYQNDKTKPKQYFIQLKTLNKGVEKTYWGADF
GELCSRESVKPGDLIRLKKLGRKEVKIPALDKNGVQHGWKMVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKAAALQSEQKLKMGIKFLGL
MGVWVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAITRQGVRNSI
DYMSRESGLPVMSESGQVWKGDEIQEAKEHMIDRANDPQYVMNDKGKENKKITQNIVFSPPVSARVKPED
LLESVRKTMHKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKRDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDVGYDHYQNDKTKPKQYFIQLKTLNKGVEKTYWGADF
GELCSRESVKPGDLIRLKKLGRKEVKIPALDKNGVQHGWKMVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKAAALQSEQKLKMGIKFLGL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 4664 | GenBank | WP_071678427 |
| Name | t4cp2_CHD54_RS22925_pSA20080453.1 |
UniProt ID | _ |
| Length | 611 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 611 a.a. Molecular weight: 69357.22 Da Isoelectric Point: 6.9118
>WP_071678427.1 type IV secretory system conjugative DNA transfer family protein [Salmonella enterica]
MSLKLPDKGQWAFIGLVMCLVTYYAGSVAVYFLNGKTLLYIWNNFDSMLLWKIITESYIRTDIRLTAIPS
LLSGMVISVTVPVFIIWQLNKTDVALYGDAKFASDSDLKKSKLLKWETKNDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLDNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKSRGRSTSQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPY
FMDRLMKVSPKLASLTMELNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL
MSLKLPDKGQWAFIGLVMCLVTYYAGSVAVYFLNGKTLLYIWNNFDSMLLWKIITESYIRTDIRLTAIPS
LLSGMVISVTVPVFIIWQLNKTDVALYGDAKFASDSDLKKSKLLKWETKNDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLDNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKSRGRSTSQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPY
FMDRLMKVSPKLASLTMELNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 21675..31665
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CHD54_RS22895 (CHD54_23335) | 16726..17181 | - | 456 | WP_134954075 | hypothetical protein | - |
| CHD54_RS22900 (CHD54_23340) | 17239..18429 | - | 1191 | WP_134954076 | YadA-like family protein | - |
| CHD54_RS22905 (CHD54_23345) | 18462..18767 | - | 306 | WP_134954077 | hypothetical protein | - |
| CHD54_RS22915 (CHD54_23355) | 19128..19430 | - | 303 | WP_071645801 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| CHD54_RS22920 (CHD54_23360) | 19427..19840 | - | 414 | WP_134954078 | cag pathogenicity island Cag12 family protein | - |
| CHD54_RS22925 (CHD54_23365) | 19837..21672 | - | 1836 | WP_071678427 | type IV secretory system conjugative DNA transfer family protein | - |
| CHD54_RS22930 (CHD54_23370) | 21675..22706 | - | 1032 | WP_071645798 | P-type DNA transfer ATPase VirB11 | virB11 |
| CHD54_RS22935 (CHD54_23375) | 22708..23913 | - | 1206 | WP_071678426 | VirB10/TraB/TrbI family type IV secretion system protein | virB10 |
| CHD54_RS22940 (CHD54_23380) | 23910..24839 | - | 930 | WP_134954079 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| CHD54_RS22945 (CHD54_23385) | 24844..25557 | - | 714 | WP_071645795 | type IV secretion system protein | virB8 |
| CHD54_RS22950 (CHD54_23390) | 25807..26901 | - | 1095 | WP_134954080 | type IV secretion system protein | virB6 |
| CHD54_RS22955 (CHD54_23395) | 26910..27197 | - | 288 | WP_134954081 | EexN family lipoprotein | - |
| CHD54_RS22960 (CHD54_23400) | 27209..27958 | - | 750 | WP_134954082 | type IV secretion system protein | - |
| CHD54_RS22965 (CHD54_23405) | 27969..30722 | - | 2754 | WP_134954083 | VirB3 family type IV secretion system protein | virb4 |
| CHD54_RS22970 (CHD54_23410) | 30741..31037 | - | 297 | WP_071645790 | TrbC/VirB2 family protein | virB2 |
| CHD54_RS22975 (CHD54_23415) | 31015..31665 | - | 651 | WP_071678421 | lytic transglycosylase domain-containing protein | virB1 |
| CHD54_RS22980 (CHD54_23420) | 31698..31910 | - | 213 | WP_071645788 | hypothetical protein | - |
| CHD54_RS22985 (CHD54_23425) | 31873..32355 | - | 483 | WP_071645787 | transcription termination/antitermination NusG family protein | - |
| CHD54_RS22990 (CHD54_23430) | 32708..33874 | - | 1167 | WP_071678439 | MobP1 family relaxase | - |
| CHD54_RS22995 (CHD54_23435) | 33877..34422 | - | 546 | WP_071645786 | DNA distortion polypeptide 1 | - |
| CHD54_RS23000 (CHD54_23440) | 34781..35128 | - | 348 | WP_071645785 | type II toxin-antitoxin system HicB family antitoxin | - |
| CHD54_RS23005 (CHD54_23445) | 35125..35373 | - | 249 | WP_071645784 | type II toxin-antitoxin system HicA family toxin | - |
| CHD54_RS23010 (CHD54_23450) | 35523..35795 | - | 273 | WP_071645783 | hypothetical protein | - |
| CHD54_RS23260 (CHD54_23455) | 35808..35957 | - | 150 | WP_167668528 | hypothetical protein | - |
| CHD54_RS23015 (CHD54_23460) | 36028..36207 | - | 180 | WP_071645782 | hypothetical protein | - |
| CHD54_RS23020 (CHD54_23465) | 36250..36579 | - | 330 | WP_071678420 | hypothetical protein | - |
Host bacterium
| ID | 7360 | GenBank | NZ_CP030195 |
| Plasmid name | pSA20080453.1 | Incompatibility group | - |
| Plasmid size | 38923 bp | Coordinate of oriT [Strand] | 34200..34615 [+] |
| Host baterium | Salmonella enterica strain SA20080453 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |