Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106920
Name   oriT_RMCH-M23-N|unnamed1 in_silico
Organism   Enterobacter cloacae strain RMCH-M23-N
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP123599 (93617..93871 [-], 255 nt)
oriT length   255 nt
IRs (inverted repeats)      153..158, 160..165  (AAAAGT..ACTTTT)
 122..128, 137..143  (TTAAGGC..GCCTTAA)
 49..55, 60..66  (GAATTTT..AAAATTC)
Location of nic site      78..79
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 255 nt

>oriT_RMCH-M23-N|unnamed1
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCATATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4575 GenBank   WP_280597626
Name   mobH_QFB83_RS23775_RMCH-M23-N|unnamed1 insolico UniProt ID   _
Length   1050 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1050 a.a.        Molecular weight: 118079.47 Da        Isoelectric Point: 4.6054

>WP_280597626.1 MobH family relaxase [Enterobacter cloacae]
MMMNFRALYLCIKRILGTFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNTKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDAITKITDT
VETAVLKVNDLGRSSASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDATMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEEEDEEHEIIDYTDFTQLSVSRPEVG
SCATSSSVHNEKLLPEPSELPELNREQNADPQGTIERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPLLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFATTESDQNGEFSLISEGDVTELEFVEIALVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(108-318)


  Protein structure



No available structure.




T4CP

ID   4663 GenBank   WP_001284076
Name   traD_QFB83_RS23780_RMCH-M23-N|unnamed1 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 78137.93 Da        Isoelectric Point: 8.0177

>WP_001284076.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANETLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

  Protein domains


Predicted by InterproScan.

AAA-like domain (AAA_10)

(199-249)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(406-609)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 168926..178133

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QFB83_RS24165 (QFB83_24165) 164033..164749 - 717 WP_047745022 zinc finger-like domain-containing protein -
QFB83_RS24170 (QFB83_24170) 164746..164946 - 201 WP_000594930 hypothetical protein -
QFB83_RS24175 (QFB83_24175) 164966..166018 - 1053 WP_280597586 hypothetical protein -
QFB83_RS24180 (QFB83_24180) 166495..167370 + 876 WP_000594612 RepB family plasmid replication initiator protein -
QFB83_RS24185 (QFB83_24185) 168522..168875 + 354 WP_000423602 hypothetical protein -
QFB83_RS24190 (QFB83_24190) 168926..169243 + 318 WP_000043359 type IV conjugative transfer system protein TraL traL
QFB83_RS24195 (QFB83_24195) 169255..170043 + 789 WP_020833712 TraE/TraK family type IV conjugative transfer system protein traE
QFB83_RS24200 (QFB83_24200) 170043..171314 + 1272 WP_280597587 type-F conjugative transfer system secretin TraK traK
QFB83_RS24205 (QFB83_24205) 171316..171777 + 462 WP_251254407 plasmid transfer protein HtdO -
QFB83_RS24210 (QFB83_24210) 171767..173122 + 1356 WP_280597588 IncHI-type conjugal transfer protein TrhB traB
QFB83_RS24215 (QFB83_24215) 173130..173612 + 483 WP_280597589 transcriptional regulator -
QFB83_RS24220 (QFB83_24220) 173731..174483 + 753 WP_182911723 protein-disulfide isomerase HtdT -
QFB83_RS24225 (QFB83_24225) 174493..175443 + 951 WP_050863229 IncHI-type conjugal transfer lipoprotein TrhV traV
QFB83_RS24230 (QFB83_24230) 175452..178133 + 2682 WP_280597591 TraC family protein virb4
QFB83_RS24235 (QFB83_24235) 181723..182976 + 1254 WP_001050364 ParA family protein -

Host bacterium

ID   7357 GenBank   NZ_CP123599
Plasmid name   RMCH-M23-N|unnamed1 Incompatibility group   IncHI2
Plasmid size   212845 bp Coordinate of oriT [Strand]   93617..93871 [-]
Host baterium   Enterobacter cloacae strain RMCH-M23-N

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   terW, terZ, terA, terB, terC, terD, terE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA9