Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106909
Name   oriT1_pMS114_1 in_silico
Organism   Salmonella enterica strain MS114
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP058672 (133719..133807 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT1_pMS114_1
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4567 GenBank   WP_223229039
Name   mobH_HWV51_RS24130_pMS114_1 insolico UniProt ID   _
Length   1050 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1050 a.a.        Molecular weight: 118051.46 Da        Isoelectric Point: 4.6054

>WP_223229039.1 MobH family relaxase [Salmonella enterica]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDAITKITDT
VETAVLKVNDLGRSTASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDATMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEEEDEEHEIIDYTDFTQLSVSRLEVG
SCATSSSVHNEKLLPEPSELPELNREQNADPQGTIERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFATTESDQNGEFSLISEGDVTELEFVEIASVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(108-304)


  Protein structure



No available structure.




T4CP

ID   4654 GenBank   WP_001284076
Name   traD_HWV51_RS24125_pMS114_1 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 78137.93 Da        Isoelectric Point: 8.0177

>WP_001284076.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANETLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

  Protein domains


Predicted by InterproScan.

AAA-like domain (AAA_10)

(199-249)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(406-609)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 37311..46518

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWV51_RS23550 37311..39992 - 2682 WP_244780216 TraC family protein virb4
HWV51_RS23555 40001..40951 - 951 WP_001022587 IncHI-type conjugal transfer lipoprotein TrhV traV
HWV51_RS23560 40961..41713 - 753 WP_183079335 protein-disulfide isomerase HtdT -
HWV51_RS23565 41832..42314 - 483 WP_000377633 hypothetical protein -
HWV51_RS23570 42322..43677 - 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
HWV51_RS23575 43667..44128 - 462 WP_000521240 plasmid transfer protein HtdO -
HWV51_RS23580 44130..45401 - 1272 WP_000592090 type-F conjugative transfer system secretin TraK traK
HWV51_RS23585 45401..46189 - 789 WP_000783153 TraE/TraK family type IV conjugative transfer system protein traE
HWV51_RS23590 46201..46518 - 318 WP_000043356 type IV conjugative transfer system protein TraL traL
HWV51_RS23595 46569..46922 - 354 WP_000423602 hypothetical protein -
HWV51_RS23600 48071..48946 - 876 WP_000594612 RepB family plasmid replication initiator protein -
HWV51_RS23605 49423..50478 + 1056 WP_001065779 hypothetical protein -
HWV51_RS23610 50496..50696 + 201 WP_000594931 hypothetical protein -
HWV51_RS23615 50693..51409 + 717 WP_000838218 zinc finger-like domain-containing protein -

Host bacterium

ID   7346 GenBank   NZ_CP058672
Plasmid name   pMS114_1 Incompatibility group   IncHI2A
Plasmid size   242694 bp Coordinate of oriT [Strand]   133719..133807 [-]; 154497..154750 [+]
Host baterium   Salmonella enterica strain MS114

Cargo genes

Drug resistance gene   aph(3'')-Ib, aph(6)-Id
Virulence gene   iutA, iucD, iucC, iucB, iucA
Metal resistance gene   terW, silE, silS, silR, silC, silF, silB, silA, silP, pcoA, pcoB, pcoC, pcoD, pcoR, pcoS, pcoE, terE, terD, terC, terB, terA, terZ
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -