Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 106893 |
| Name | oriT_fxq|pD  |
| Organism | Klebsiella michiganensis strain fxq |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP095003 (90463..90544 [-], 82 nt) |
| oriT length | 82 nt |
| IRs (inverted repeats) | 27..32, 35..40 (GCCACC..GGTGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 82 nt
>oriT_fxq|pD
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 4558 | GenBank | WP_064357930 |
| Name | Relaxase_MT472_RS32460_fxq|pD |
UniProt ID | _ |
| Length | 642 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 642 a.a. Molecular weight: 74304.29 Da Isoelectric Point: 8.8672
>WP_064357930.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MNAIIPPKRRDKKSSFVQLVAYVSVRDDVPLKDELKEDQRFQRPSRSRQAIFDRLVDYMNRSEGTITEQI
TDMSPEGDVRCSVDGVICQHNLMSVETAAMEMNSIAMQNLHVKDAVYHYILSWQEDENPSDDQVFDSVKY
SLKRLGMEENQYVAGIHRDTDNLHVHVAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPSMTLSRLEPALGTFVPSPRSAEMVKQSVQTPYSDKLHVRDRGA
RAKRRQARADARIDLRERYGKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASENI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGCFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDERYYD
DVRNNDDSSYKP
MNAIIPPKRRDKKSSFVQLVAYVSVRDDVPLKDELKEDQRFQRPSRSRQAIFDRLVDYMNRSEGTITEQI
TDMSPEGDVRCSVDGVICQHNLMSVETAAMEMNSIAMQNLHVKDAVYHYILSWQEDENPSDDQVFDSVKY
SLKRLGMEENQYVAGIHRDTDNLHVHVAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPSMTLSRLEPALGTFVPSPRSAEMVKQSVQTPYSDKLHVRDRGA
RAKRRQARADARIDLRERYGKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASENI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGCFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDERYYD
DVRNNDDSSYKP
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 4642 | GenBank | WP_064357960 |
| Name | trbC_MT472_RS32315_fxq|pD |
UniProt ID | _ |
| Length | 730 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 730 a.a. Molecular weight: 82695.78 Da Isoelectric Point: 6.0387
>WP_064357960.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MQQKQKPVDAARLQRQVHQSMVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLI
LMATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFLWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTIIDPTETAEFAAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALN
QGEQATVFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMA
IVKNDNIRHAPDSMIIQDGVLYAIADEAMYFDEVSPVPPDTVRRATQLLSLAASVLTETRGRYSVEYPEP
EKLSVKKDELEKYQHIHQSAVTQQVDGFVY
MQQKQKPVDAARLQRQVHQSMVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLI
LMATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFLWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTIIDPTETAEFAAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALN
QGEQATVFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMA
IVKNDNIRHAPDSMIIQDGVLYAIADEAMYFDEVSPVPPDTVRRATQLLSLAASVLTETRGRYSVEYPEP
EKLSVKKDELEKYQHIHQSAVTQQVDGFVY
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 58623..80348
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MT472_RS32300 (MT472_32300) | 54381..54878 | - | 498 | WP_159041655 | RepA family replication protein | - |
| MT472_RS32305 (MT472_32305) | 55587..55838 | - | 252 | WP_064357964 | hypothetical protein | - |
| MT472_RS32310 (MT472_32310) | 55850..56383 | - | 534 | WP_096807525 | phospholipase D family protein | - |
| MT472_RS32315 (MT472_32315) | 56429..58621 | - | 2193 | WP_064357960 | F-type conjugative transfer protein TrbC | - |
| MT472_RS32320 (MT472_32320) | 58623..59714 | - | 1092 | WP_064357959 | thioredoxin fold domain-containing protein | trbB |
| MT472_RS32325 (MT472_32325) | 59725..61143 | - | 1419 | WP_071887238 | hypothetical protein | trbA |
| MT472_RS32330 (MT472_32330) | 61245..61868 | - | 624 | WP_064357957 | plasmid IncI1-type surface exclusion protein ExcA | - |
| MT472_RS32335 (MT472_32335) | 61934..64078 | - | 2145 | WP_064357955 | DotA/TraY family protein | traY |
| MT472_RS32340 (MT472_32340) | 64120..64701 | - | 582 | WP_064357954 | conjugal transfer protein TraX | - |
| MT472_RS32345 (MT472_32345) | 64698..65894 | - | 1197 | WP_064357952 | conjugal transfer protein TraW | traW |
| MT472_RS32350 (MT472_32350) | 65904..68951 | - | 3048 | WP_189095616 | ATP-binding protein | traU |
| MT472_RS32355 (MT472_32355) | 68961..69524 | - | 564 | WP_064357949 | hypothetical protein | traT |
| MT472_RS32360 (MT472_32360) | 69568..69969 | - | 402 | WP_032440588 | DUF6750 family protein | traR |
| MT472_RS32365 (MT472_32365) | 70025..70558 | - | 534 | WP_064357947 | conjugal transfer protein TraQ | traQ |
| MT472_RS32370 (MT472_32370) | 70568..71287 | - | 720 | WP_064357945 | conjugal transfer protein TraP | traP |
| MT472_RS32375 (MT472_32375) | 71294..72622 | - | 1329 | WP_064357943 | conjugal transfer protein TraO | traO |
| MT472_RS32380 (MT472_32380) | 72626..73729 | - | 1104 | WP_081042499 | DotH/IcmK family type IV secretion protein | traN |
| MT472_RS32385 (MT472_32385) | 73740..74408 | - | 669 | WP_196548568 | DotI/IcmL/TraM family protein | traM |
| MT472_RS32390 (MT472_32390) | 74482..74835 | - | 354 | WP_064357942 | hypothetical protein | traL |
| MT472_RS32395 (MT472_32395) | 74869..78084 | - | 3216 | WP_064357941 | LPD7 domain-containing protein | - |
| MT472_RS32400 (MT472_32400) | 78102..78368 | - | 267 | WP_189095618 | IcmT/TraK family protein | traK |
| MT472_RS32405 (MT472_32405) | 78365..79552 | - | 1188 | WP_081042498 | plasmid transfer ATPase TraJ | - |
| MT472_RS32410 (MT472_32410) | 79530..80348 | - | 819 | WP_064357938 | type IV secretory system conjugative DNA transfer family protein | traI |
| MT472_RS32415 (MT472_32415) | 80345..80809 | - | 465 | WP_225546581 | DotD/TraH family lipoprotein | - |
| MT472_RS32420 (MT472_32420) | 80835..81314 | - | 480 | WP_064357937 | lytic transglycosylase domain-containing protein | - |
| MT472_RS32425 (MT472_32425) | 81327..81695 | - | 369 | WP_136129316 | hypothetical protein | - |
| MT472_RS32430 (MT472_32430) | 82046..82273 | - | 228 | WP_032440573 | hypothetical protein | - |
| MT472_RS32435 (MT472_32435) | 82883..83725 | + | 843 | WP_032440572 | helix-turn-helix domain-containing protein | - |
| MT472_RS32440 (MT472_32440) | 83785..84714 | + | 930 | WP_064357981 | hypothetical protein | - |
| MT472_RS32445 (MT472_32445) | 84707..85201 | + | 495 | WP_064357935 | molybdopterin-dependent oxidoreductase | - |
Host bacterium
| ID | 7330 | GenBank | NZ_CP095003 |
| Plasmid name | fxq|pD | Incompatibility group | IncFII |
| Plasmid size | 107094 bp | Coordinate of oriT [Strand] | 90463..90544 [-] |
| Host baterium | Klebsiella michiganensis strain fxq |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |