Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 106818
• Name: oriT_pSme442-SymB
• Organism: Sinorhizobium meliloti strain BIM B-442D
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP123006 (708699..708727 [+], 29 nt)
• oriT length: 29 nt
• IRs (inverted repeats): 14..19, 24..29 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 29 nt

>oriT_pSme442-SymB
AGGGCGCAATATACGTCGCTGGCGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_QC756_RS30630_pSme442-SymB

• ID: 4494
• GenBank: WP_274885102
• Name: traA_QC756_RS30630_pSme442-SymB
• UniProt ID: _
• Length: 1539 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1539 a.a.
• Molecular weight: 169725.34 Da
• Isoelectric Point: 9.4871

>WP_274885102.1 Ti-type conjugative transfer relaxase TraA [Sinorhizobium meliloti]
MAIMFVRAQVIGRGAGRSIVSAAAYRHRTRMIDEQAGTSFSYRGGASELVHEELALPDDIPAWLKAAIDG
QSVAKASEALWNAVEAHETRADAQLARELIIALPEELTRAENIALVREFVRDNLTSKGMVADWVYHDKDG
NPHIHLMTALRPLTEQGFGPKKVPVLGEDGEPLRVVTPDRPNGKIVYKLWAGDKETIKAWKIAWAETANR
HLALAGHEIRLDGRSYAEQGLDGIAQKHLGPEKAALARKGIAMYFAPADLARRQEMADRLLAEPGLLLKQ
LGNERSTFDERDIAKALHRYVDDPVDFANIRARLMASDDLVLLKPQQVDAETGEAKQPAVFTTREMLRLE
YAMARSAEVLSRRKGFGVSNARAAAAVRSIETADTEKPFRLDPEQVDAVRHVTRDNAIAAVVGLAGAGKS
TLLAAARVAWEGEGRRVIGAALAGKAAEGLEDSSGIRSRTLASWELAWESGRKQLNRGDVLVIDEAGMVS
SQQMARILKAVEDAGAKAVLVGDAMQLQPIEAGAAFRAITERIGFAELAGVRRQRDAWARDASRLFARGK
VEEGLDAYAQHGRIVETETRAKIVDRIVADWADARRDLLQKSADGEHPGRLRGDELLVLAHTNDDVRKLN
TSLRNVIVGEGALAGAREFQTARGLREFAAGDRIIFLENARFVEPRARRLGPQYVKNGMLGTVVSTGDRR
GDTLLSVRLDSGRDVVISEDSYRNVDHGYAATIHKSQGSTVDRTFVLATGMMDQHLTYVAMTRHRDRADL
YAAKEDFEPKPEWGRKPRVDHAAGVTGELVEEGMAKFRPNDEDADESPYADIRTDDGTVHRLWGVSLPKA
LKGAGAAEGDTITLRKDGVERVKVQVPIVDAQTGEKRFEERQVDRNVWSASQLETAAARRERIERESHRP
QLFAALVERLSRSGAKTTTLDFEDEAGYQAQARDFARRRGLYHLSLVAAGMEAEVLRRWAGIAEKREQVA
KLWERASVALGFAIERERRVAYNEERTETLSTGIPSDGKYLVPPTTTFSRSVAEDARLAQLSSQRWKERE
AIVHPVLAKIYRDPDGALSALNALASDAAIEPRKLAEDLGLAPERLGRLRGSELVVDGRAARDERTAATA
ALSELLPLARAHATEFRRNAERFGIREQQRRAHMALSVPALSKTAMARLVEIEAVREQGGDDAYRTAFAY
AVEDRLLVQEVKAVNEALTARFGWSAFTAKADVIAERNMSERMPEDLAPERREKLTRLFAVIRRFAEEQH
LAERQDRSKIVAGASVELGKETFAVLPMLAAVTEFKTTVDEEARERALAAPHYAHHRAALVETATRVWRD
PADAIGKIEDLIVKGFAGERIAAAVTNDPAAYGALRGSDRIMDKLLAVGRERKGALQAVPEAASRIRSLG
ASYASALDAETRSITEERRRMAVAIPGLSPAAEDALKRLAAQIKNKDGKLDVAAGSLDPRIAREFAKVSR
ALDDRFGRNAILRGETDVINRVSPAQRRAFEAMRDRLQVLQQAVRIQSSQKIVSERRQRAINQSRGIRM

Protein domains

Predicted by InterproScan.

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1309-1400)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1047-1125)

• AAA domain (AAA_30) (391-578)

• MobA/MobL family (MobA_MobL) (17-249)

Protein structure

No available structure.

T4CP

tcpA_QC756_RS34640_pSme442-SymB

• ID: 4550
• GenBank: WP_014530096
• Name: tcpA_QC756_RS34640_pSme442-SymB
• UniProt ID: _
• Length: 946 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 946 a.a.
• Molecular weight: 101605.36 Da
• Isoelectric Point: 4.5919

>WP_014530096.1 FtsK/SpoIIIE domain-containing protein [Sinorhizobium meliloti]
MRIPRTNFSPAALYDANHADDYEDPHPAAQGAAHVPAAPEAAAHDHLFEAPEAPRRAPGHRDEETGYAGR
AARLYGHGSAYAPAEVTVSTRDPLPTLAEITGLSGEPGWESHFFLSPNVRFTRTPERELMKRHPPAPEES
RIEADEAAAEASDAETVMDVVPAEPAPSVVETELPSYSPSELLRVLTQQLPSWSAARSQAPEASVTKPAI
TESVAVAEEGPATSETTALPVTDEVPVVPHALPVANLAPESAAVEEDVPHQADARLAYLSDFAFFEFMPL
EVAAVPPTVTEPVKEAARIPAPIAAAPKVSPPKIVAAMPVEIRPQPPTAISSLFRVVECRRPEPATAEPV
TAAPQDIAAEPAAAEAAALPAHQFVETPAPALAEPAIVPASEPAPEAPVTRAAITMPAVIQRSSPSLPPI
GAIEPLQGGDAYEFPSKELLQEPPQGQGFFMTQEQLEQNAGLLESVLEDFGVKGEIIHVRPGPVVTLYEF
EPAPGVKSSRVIGLADDIARSMSALSARVAVVPGRNVIGIELPNATRETVYFRELIESGDFQKTGCKLAL
CLGKTIGGEPVIAELAKMPHLLVAGTTGSGKSVAINTMILSLLYRLKPEECRLIMVDPKMLELSVYDGIP
HLLTPVVTDPKKAVMALKWAVREMEDRYRKMSRLGVRNIDGYNQRAAAAREKGAPILATVQTGFEKGTGE
PLFEQQEMDLSPMPYIVVIVDEMADLMMVAGKEIEGAIQRLAQMARAAGIHLIMATQRPSVDVITGTIKA
NFPTRISFQVTSKIDSRTILGEQGAEQLLGQGDMLHMAGGGRIARVHGPFVSDQEVEHVVAHLKTQGRPE
YLETVTADEEEEEVEEDQGAVFDKSAIAAEDGNELYDQAVKVVLRDKKCSTSYIQRRLGIGYNRAASLVE
RMEKDGLVGPANHVGKREIIYGNRDNAPKPESDDLD

Protein domains

Predicted by InterproScan.

• FtsK/SpoIIIE family (FtsK_SpoIIIE) (543-761)

• Ftsk gamma domain (FtsK_gamma) (871-930)

• FtsK alpha domain (FtsK_alpha) (434-534)

Protein structure

No available structure.

Host bacterium

• ID: 7255
• GenBank: NZ_CP123006
• Plasmid name: pSme442-SymB
• Incompatibility group: -
• Plasmid size: 1662188 bp
• Coordinate of oriT [Strand]: 708699..708727 [+]
• Host baterium: Sinorhizobium meliloti strain BIM B-442D

Cargo genes

• Drug resistance gene: -
• Virulence gene: htpB
• Metal resistance gene: actP
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA7, AcrVIB