oriTDB

Detailed information of oriT

oriT
Relaxase
Auxiliary protein
T4CP
T4SS
Host bacterium
Cargo genes

oriT

The information of the oriT region

oriTDB ID	106771
Name	oriT_pESA136_4
Organism	Escherichia albertii strain Sample 165
Sequence Completeness	-
*NCBI accession of oriT* (coordinates [strand])**	NZ_CP070300 (62323..62446 [-], 124 nt)
oriT length	124 nt
IRs (inverted repeats)	101..106, 119..124 (TTTAAT..ATTAAA) 91..99, 113..121 (AATAATGTA..TACATTATT) 90..95, 107..112 (AAATAA..TTATTT) 39..46, 49..56 (GCAAAAAC..GTTTTTGC) 3..10, 15..22 (TTGGTGGT..ACCACCAA)
*Location of nic* site**	_
Conserved sequence flanking the nic site	_
Note	Predicted by oriTfinder 2.0

oriT sequence

Download Length: 124 nt

                        >oriT_pESA136_4
GGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTGCTTTTTGAATCATTAGCTTATGTTTTAAATAATGTATTTTAATTTATTTTACATTATTAAA                      

Visualization of oriT structure

oriT secondary structure

Predicted by RNAfold.

Download structure file

Relaxase

traI_JRC44_RS25880_pESA136_4

ID	4463	GenBank	WP_061093447
Name	traI_JRC44_RS25880_pESA136_4	UniProt ID	A0A2Z4I1F4
Length	1756 a.a.	PDB ID
Note	Predicted by oriTfinder 2.0

Relaxase protein sequence

Download Length: 1756 a.a. Molecular weight: 192119.90 Da Isoelectric Point: 6.0788

          >WP_061093447.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]

MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ

DGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV

MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA

LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT

LKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE

NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS

DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR

RQLPEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNIYRW

QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT

MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDVQGETQVVRISSLDS

SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDVMTVVVPGRAEPASLPVSDSPFTALKL

ENGWVETPGHSVSDSAKVFASVTQMAMDKATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI

KARAGETSLETAISHQKSALNTPAQQAIHLALPVVESKNLAFSHVDLLTEAKSFAAEGTSFADLGREIDA

QIKRGDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI

LETPDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT

QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA

ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA

QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM

VPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISRDDGLITLQDAEGNTRLISPREAVAEGVT

LYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPAQEQAEQHIDLAYAI

TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL

EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG

IWLNPLTSDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE

GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP

DGKTEQAVREIAGQVRERAAITERETALPESVLREPQREREAVREVARENLLQERLQQMERDMVRDLQKE

KTLGGD

Protein domains

Predicted by InterproScan.

DNA helicase TraI (TraI_C)

(1434-1557)

TrwC relaxase (TrwC)

(10-283)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-626)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)

AAA domain (AAA_30)

(969-1156)

Protein structure

No available structure.

ID	1984	GenBank	WP_001254386
Name	WP_001254386_pESA136_4	UniProt ID	A0A3Z6KJB5
Length	75 a.a.	PDB ID	_
Note	Predicted by oriTfinder 2.0

Auxiliary protein sequence

Download Length: 75 a.a. Molecular weight: 9005.11 Da Isoelectric Point: 10.1422

                        >WP_001254386.1 MULTISPECIES: conjugal transfer relaxosome protein TraY [Gammaproteobacteria]

MRRRNARGGISRTVSVYLDEDTNNRLIKAKDRSGRSKTIEVQIRLRDHLKRFPDFYNEEIFREVTEESES

TFKEL

Protein domains

Predicted by InterproScan.

TraY domain (TraY)

(14-61)

Protein structure

Source	ID	Structure
AlphaFold DB	A0A3Z6KJB5

ID	1985	GenBank	WP_000124981
Name	WP_000124981_pESA136_4	UniProt ID	A0A630FTW9
Length	127 a.a.	PDB ID	_
Note	Predicted by oriTfinder 2.0

Auxiliary protein sequence

Download Length: 127 a.a. Molecular weight: 14542.59 Da Isoelectric Point: 5.0278

                        >WP_000124981.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Gammaproteobacteria]

MARVNLYISNEVHEKINMIVEKRRQEGARDKDISLSGTASMLLELGLRVYDAQMERKESAFNQTEFNKLL

LECVVKTQSTVAKILGIESLSPHVSGNPKFEYASMVDDIREKVSVEMDRFFPKNDDE

Protein domains

Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-126)

Protein structure

Source	ID	Structure
AlphaFold DB	A0A630FTW9

T4CP

traD_JRC44_RS25885_pESA136_4
traC_JRC44_RS25990_pESA136_4

ID	4510	GenBank	WP_021518883
Name	traD_JRC44_RS25885_pESA136_4	UniProt ID	_
Length	726 a.a.	PDB ID	_
Note	Predicted by oriTfinder 2.0

T4CP protein sequence

Download Length: 726 a.a. Molecular weight: 82493.06 Da Isoelectric Point: 5.0504

                        >WP_021518883.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]

MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCIYWWCTTLEGMR

DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQ

QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR

QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG

SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA

IRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV

WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA

EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL

KYQARPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPQQPQQ

PQQPVSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQQENHPDIQQ

QMQRREEVNINVHRERGEDVEPGDDF

Protein domains

Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(173-560)

Protein structure

No available structure.

ID	4511	GenBank	WP_112929298
Name	traC_JRC44_RS25990_pESA136_4	UniProt ID	_
Length	875 a.a.	PDB ID	_
Note	Predicted by oriTfinder 2.0

T4CP protein sequence

Download Length: 875 a.a. Molecular weight: 99397.23 Da Isoelectric Point: 6.3465

                        >WP_112929298.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacteriaceae]

MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP

INGANESIVEALDHMLRTKLPRGVPFCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAA

TQFPLPEGMNLPLTLRHYRVFFSYCSPSKKKSRADILEMENLVKIIRASLQGASITTQAVDAQAFIDIVG

EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAFL

WNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE

LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG

LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGA

GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV

MASPNGNLDEVHEGLLLQAVRASWLAKKKQARIDDVVDFLKNARDNDQYVESPTIRSRLDEMIVLLDQYT

ANGTYGRYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRTLKKLNVIDEGWR

LLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN

QLFPDQFQPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGM

SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

Protein domains

Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(290-446)

TraC protein (TraC_F_IV)

(38-276)

TraG P-loop domain (P-loop_TraG)

(467-771)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 34875..63018

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRC44_RS25885 (JRC44_25885)	34875..37055	-	2181	WP_021518883	type IV conjugative transfer system coupling protein TraD	virb4
JRC44_RS25890 (JRC44_25890)	37106..37843	-	738	WP_015059020	hypothetical protein	-
JRC44_RS25895 (JRC44_25895)	38046..38777	-	732	WP_021559024	conjugal transfer complement resistance protein TraT	-
JRC44_RS25900 (JRC44_25900)	38802..39323	-	522	WP_000632668	conjugal transfer entry exclusion protein TraS	-
JRC44_RS25905 (JRC44_25905)	39356..42172	-	2817	WP_015059018	conjugal transfer mating-pair stabilization protein TraG	traG
JRC44_RS25910 (JRC44_25910)	42169..43542	-	1374	WP_000944325	conjugal transfer pilus assembly protein TraH	traH
JRC44_RS25915 (JRC44_25915)	43529..43921	-	393	WP_000126338	F-type conjugal transfer protein TrbF	-
JRC44_RS25920 (JRC44_25920)	43875..44189	-	315	WP_001384255	P-type conjugative transfer protein TrbJ	-
JRC44_RS25925 (JRC44_25925)	44179..44724	-	546	WP_000059817	type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB	traF
JRC44_RS25930 (JRC44_25930)	44711..44947	-	237	WP_000270010	type-F conjugative transfer system pilin chaperone TraQ	-
JRC44_RS25935 (JRC44_25935)	45108..45851	-	744	WP_001030371	type-F conjugative transfer system pilin assembly protein TraF	traF
JRC44_RS25940 (JRC44_25940)	45844..46101	-	258	WP_000864320	conjugal transfer protein TrbE	-
JRC44_RS25945 (JRC44_25945)	46128..47978	-	1851	WP_015059015	type-F conjugative transfer system mating-pair stabilization protein TraN	traN
JRC44_RS25950 (JRC44_25950)	47975..48397	-	423	WP_001229309	HNH endonuclease signature motif containing protein	-
JRC44_RS25955 (JRC44_25955)	48423..48794	-	372	WP_000056336	hypothetical protein	-
JRC44_RS25960 (JRC44_25960)	48791..49429	-	639	WP_000087532	type-F conjugative transfer system pilin assembly protein TrbC	trbC
JRC44_RS25965 (JRC44_25965)	49456..49977	-	522	WP_015059014	hypothetical protein	-
JRC44_RS25970 (JRC44_25970)	50040..50348	-	309	WP_000412447	hypothetical protein	-
JRC44_RS25975 (JRC44_25975)	50375..51367	-	993	WP_000830838	conjugal transfer pilus assembly protein TraU	traU
JRC44_RS25980 (JRC44_25980)	51364..51996	-	633	WP_001203720	type-F conjugative transfer system protein TraW	traW
JRC44_RS25985 (JRC44_25985)	51993..52379	-	387	WP_000214082	type-F conjugative transfer system protein TrbI	-
JRC44_RS25990 (JRC44_25990)	52376..55003	-	2628	WP_112929298	type IV secretion system protein TraC	virb4
JRC44_RS25995 (JRC44_25995)	55163..55384	-	222	WP_001278692	conjugal transfer protein TraR	-
JRC44_RS26000 (JRC44_26000)	55519..56034	-	516	WP_000809902	type IV conjugative transfer system lipoprotein TraV	traV
JRC44_RS26005 (JRC44_26005)	56031..56282	-	252	WP_001038341	conjugal transfer protein TrbG	-
JRC44_RS26010 (JRC44_26010)	56294..56491	-	198	WP_001324648	conjugal transfer protein TrbD	-
JRC44_RS26015 (JRC44_26015)	56478..57068	-	591	WP_000002778	conjugal transfer pilus-stabilizing protein TraP	-
JRC44_RS26020 (JRC44_26020)	57058..58485	-	1428	WP_032297072	F-type conjugal transfer pilus assembly protein TraB	traB
JRC44_RS26025 (JRC44_26025)	58485..59213	-	729	WP_001230787	type-F conjugative transfer system secretin TraK	traK
JRC44_RS26030 (JRC44_26030)	59200..59766	-	567	WP_000399794	type IV conjugative transfer system protein TraE	traE
JRC44_RS26035 (JRC44_26035)	59788..60099	-	312	WP_000012106	type IV conjugative transfer system protein TraL	traL
JRC44_RS26040 (JRC44_26040)	60114..60479	-	366	WP_021519752	type IV conjugative transfer system pilin TraA	-
JRC44_RS26045 (JRC44_26045)	60513..60740	-	228	WP_001254386	conjugal transfer relaxosome protein TraY	-
JRC44_RS26050 (JRC44_26050)	60834..61520	-	687	WP_015059009	PAS domain-containing protein	-
JRC44_RS26055 (JRC44_26055)	61711..62094	-	384	WP_000124981	conjugal transfer relaxosome DNA-binding protein TraM	-
JRC44_RS26060 (JRC44_26060)	62371..63018	+	648	WP_015059008	transglycosylase SLT domain-containing protein	virB1
JRC44_RS26065 (JRC44_26065)	63315..64136	-	822	WP_001234445	DUF932 domain-containing protein	-
JRC44_RS26070 (JRC44_26070)	64247..64543	-	297	WP_001272251	hypothetical protein	-
JRC44_RS26740	64843..65316	+	474	Protein_79	hypothetical protein	-
JRC44_RS26080 (JRC44_26080)	65402..65965	-	564	WP_015059007	class I SAM-dependent methyltransferase	-
JRC44_RS26085 (JRC44_26085)	66012..67373	-	1362	WP_000170695	DUF3560 domain-containing protein	-
JRC44_RS26090 (JRC44_26090)	67425..67655	-	231	WP_000218642	hypothetical protein	-

Host bacterium

ID	7208	GenBank	NZ_CP070300
Plasmid name	pESA136_4	Incompatibility group	IncFII
Plasmid size	97847 bp	Coordinate of oriT [Strand]	62323..62446 [-]
Host baterium	Escherichia albertii strain Sample 165

Cargo genes

Drug resistance gene	blaCTX-M-55, blaTEM-1B, fosA3
Virulence gene	-
Metal resistance gene	-
Degradation gene	-
Symbiosis gene	-
Anti-CRISPR	-