Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106770
Name   oriT_pESA136_3 in_silico
Organism   Escherichia albertii strain Sample 165
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP070299 (45293..45380 [-], 88 nt)
oriT length   88 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 88 nt

>oriT_pESA136_3
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4462 GenBank   WP_011264053
Name   nikB_JRC44_RS25515_pESA136_3 insolico UniProt ID   A0A767TAZ8
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103882.34 Da        Isoelectric Point: 7.4198

>WP_011264053.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A767TAZ8


Auxiliary protein

ID   1983 GenBank   WP_001283947
Name   WP_001283947_pESA136_3 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   4509 GenBank   WP_072107381
Name   trbC_JRC44_RS25520_pESA136_3 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86883.07 Da        Isoelectric Point: 6.7712

>WP_072107381.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTAKTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKIDVQELIELH
PGEFFSIFRGETVPSASFFIPDNEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTAQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 50758..81323

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JRC44_RS25520 (JRC44_25520) 48474..50765 - 2292 WP_072107381 F-type conjugative transfer protein TrbC -
JRC44_RS25525 (JRC44_25525) 50758..51828 - 1071 WP_000151579 IncI1-type conjugal transfer protein TrbB trbB
JRC44_RS25530 (JRC44_25530) 51847..53055 - 1209 WP_001383960 IncI1-type conjugal transfer protein TrbA trbA
JRC44_RS25535 (JRC44_25535) 53273..54259 - 987 WP_001257838 hypothetical protein -
JRC44_RS25540 (JRC44_25540) 54532..54684 + 153 WP_001331364 Hok/Gef family protein -
JRC44_RS25545 (JRC44_25545) 54756..55007 - 252 WP_001291964 hypothetical protein -
JRC44_RS26890 55506..55601 + 96 WP_001303310 DinQ-like type I toxin DqlB -
JRC44_RS25550 (JRC44_25550) 55666..55842 - 177 WP_001054901 hypothetical protein -
JRC44_RS25555 (JRC44_25555) 56174..56383 + 210 WP_000062602 HEAT repeat domain-containing protein -
JRC44_RS25560 (JRC44_25560) 56455..57117 - 663 WP_060527638 plasmid IncI1-type surface exclusion protein ExcA -
JRC44_RS25565 (JRC44_25565) 57188..59356 - 2169 WP_000698368 DotA/TraY family protein traY
JRC44_RS25570 (JRC44_25570) 59453..60037 - 585 WP_060527640 IncI1-type conjugal transfer protein TraX -
JRC44_RS25575 (JRC44_25575) 60066..61268 - 1203 WP_001189156 IncI1-type conjugal transfer protein TraW traW
JRC44_RS25580 (JRC44_25580) 61235..61849 - 615 WP_000337399 IncI1-type conjugal transfer protein TraV traV
JRC44_RS25585 (JRC44_25585) 61849..64893 - 3045 WP_071447696 IncI1-type conjugal transfer protein TraU traU
JRC44_RS25590 (JRC44_25590) 64983..65783 - 801 WP_060527642 IncI1-type conjugal transfer protein TraT traT
JRC44_RS25595 (JRC44_25595) 65767..65955 - 189 WP_001277255 putative conjugal transfer protein TraS -
JRC44_RS25600 (JRC44_25600) 66019..66423 - 405 WP_000086960 IncI1-type conjugal transfer protein TraR traR
JRC44_RS25605 (JRC44_25605) 66474..67001 - 528 WP_001055569 conjugal transfer protein TraQ traQ
JRC44_RS25610 (JRC44_25610) 67001..67705 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
JRC44_RS25615 (JRC44_25615) 67705..68994 - 1290 WP_001271994 conjugal transfer protein TraO traO
JRC44_RS25620 (JRC44_25620) 68997..69980 - 984 WP_021513963 IncI1-type conjugal transfer protein TraN traN
JRC44_RS25625 (JRC44_25625) 69991..70683 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
JRC44_RS25630 (JRC44_25630) 70680..71027 - 348 WP_001055900 conjugal transfer protein traL
JRC44_RS25635 (JRC44_25635) 71045..74812 - 3768 WP_021513964 LPD7 domain-containing protein -
JRC44_RS25640 (JRC44_25640) 74902..75453 - 552 WP_000014584 phospholipase D family protein -
JRC44_RS25645 (JRC44_25645) 75468..75758 - 291 WP_001299214 hypothetical protein traK
JRC44_RS25650 (JRC44_25650) 75755..76903 - 1149 WP_001024976 plasmid transfer ATPase TraJ virB11
JRC44_RS25655 (JRC44_25655) 76900..77718 - 819 WP_000646097 IncI1-type conjugal transfer lipoprotein TraI traI
JRC44_RS25660 (JRC44_25660) 77715..78173 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
JRC44_RS25665 (JRC44_25665) 78568..79152 - 585 WP_000977525 histidine phosphatase family protein -
JRC44_RS25670 (JRC44_25670) 79211..80413 - 1203 WP_000976353 conjugal transfer protein TraF -
JRC44_RS25675 (JRC44_25675) 80499..81323 - 825 WP_208629837 conjugal transfer protein TraE traE
JRC44_RS25680 (JRC44_25680) 81474..82628 - 1155 WP_001139955 site-specific integrase -

Host bacterium

ID   7207 GenBank   NZ_CP070299
Plasmid name   pESA136_3 Incompatibility group   IncI1
Plasmid size   82787 bp Coordinate of oriT [Strand]   45293..45380 [-]
Host baterium   Escherichia albertii strain Sample 165

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -