Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106769
Name   oriT_pESA136_1 in_silico
Organism   Escherichia albertii strain Sample 165
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP070297 (98188..98441 [+], 254 nt)
oriT length   254 nt
IRs (inverted repeats)      152..157, 159..164  (AAAAGT..ACTTTT)
 121..129, 134..142  (TTAAGGCTT..AAGCCTTAA)
 50..55, 59..64  (AATTTT..AAAATT)
Location of nic site      77..78
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 254 nt

>oriT_pESA136_1
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGAAGAAATTTTGATAAAATTCTGGTCAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4461 GenBank   WP_227536610
Name   mobH_JRC44_RS23835_pESA136_1 insolico UniProt ID   _
Length   1050 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1050 a.a.        Molecular weight: 117977.38 Da        Isoelectric Point: 4.6192

>WP_227536610.1 MULTISPECIES: MobH family relaxase [Enterobacteriaceae]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSGIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDAITKITDT
VETAVLKVNDLGRSTASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDATMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEEEDEEHEIIDYTDFTQLSVSRPEVG
SCATSSSVHNEKLLPEPSELPELNREQNADPQGTIERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFATTESDQNGEFSLISEGDVTELEFVEIASVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(108-304)


  Protein structure



No available structure.




T4CP

ID   4508 GenBank   WP_001284076
Name   traD_JRC44_RS23830_pESA136_1 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 78137.93 Da        Isoelectric Point: 8.0177

>WP_001284076.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANETLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

  Protein domains


Predicted by InterproScan.

AAA-like domain (AAA_10)

(199-249)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(406-609)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 10843..20050

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JRC44_RS23385 (JRC44_23385) 10843..13524 - 2682 WP_000387412 TraC family protein virb4
JRC44_RS23390 (JRC44_23390) 13533..14483 - 951 WP_001022587 IncHI-type conjugal transfer lipoprotein TrhV traV
JRC44_RS23395 (JRC44_23395) 14493..15245 - 753 WP_183079335 protein-disulfide isomerase HtdT -
JRC44_RS23400 (JRC44_23400) 15364..15846 - 483 WP_065311104 transcriptional regulator -
JRC44_RS23405 (JRC44_23405) 15854..17209 - 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
JRC44_RS23410 (JRC44_23410) 17199..17660 - 462 WP_000521240 plasmid transfer protein HtdO -
JRC44_RS23415 (JRC44_23415) 17662..18933 - 1272 WP_000592090 type-F conjugative transfer system secretin TraK traK
JRC44_RS23420 (JRC44_23420) 18933..19721 - 789 WP_000783153 TraE/TraK family type IV conjugative transfer system protein traE
JRC44_RS23425 (JRC44_23425) 19733..20050 - 318 WP_000043356 type IV conjugative transfer system protein TraL traL
JRC44_RS23430 (JRC44_23430) 20101..20454 - 354 WP_000423602 hypothetical protein -
JRC44_RS23435 (JRC44_23435) 21603..22478 - 876 WP_000594612 RepB family plasmid replication initiator protein -
JRC44_RS23440 (JRC44_23440) 22955..24010 + 1056 WP_065311103 hypothetical protein -
JRC44_RS23445 (JRC44_23445) 24028..24228 + 201 WP_000594931 hypothetical protein -
JRC44_RS23450 (JRC44_23450) 24225..24941 + 717 WP_000838218 zinc finger-like domain-containing protein -

Host bacterium

ID   7206 GenBank   NZ_CP070297
Plasmid name   pESA136_1 Incompatibility group   IncHI2A
Plasmid size   266043 bp Coordinate of oriT [Strand]   98188..98441 [+]
Host baterium   Escherichia albertii strain Sample 165

Cargo genes

Drug resistance gene   dfrA14, ARR-3, mph(A), qnrS1, blaCTX-M-55, blaTEM-1B, blaLAP-2, aac(3)-IId, ant(3'')-Ia, lnu(F), sul3, tet(A), aph(6)-Id, floR, aph(3')-Ia
Virulence gene   -
Metal resistance gene   terE, terD, terC, terB, terA, terZ, terW
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -