Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   106766
Name   oriT_XJ5|unnamed2 in_silico
Organism   Bacillus amyloliquefaciens strain XJ5
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP071972 (6..296 [+], 291 nt)
oriT length   291 nt
IRs (inverted repeats)      220..225, 231..236  (AAAAAG..CTTTTT)
 118..123, 128..133  (CACCAT..ATGGTG)
 101..108, 110..117  (AAACCGCA..TGCGGTTT)
 35..40, 47..52  (TTTATG..CATAAA)
Location of nic site      41..42
Conserved sequence flanking the
  nic site  
 
 TATGGTTACA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 291 nt

>oriT_XJ5|unnamed2
AAGATTTATGTGCTTTTTGATTTTAATCACCTAATTTATGGTTACACATAAACATTTTGTTTATGTGCATTGGGCAATAAATCTGGTACCACGACAAAACAAACCGCACTGCGGTTTCACCATGGGAATGGTGCCAGTTTCCCCTTATGCTCTTTCCTTTTCCACCCCTCCTTCCTTCGGAATCGGGGGCCGGTTTTTTGCTGCCGCAAAAAACCTTGGAAAAAGAAACACTTTTTTTGAACAGATCATTGCAAAATAAAGTGCCAAAACAGATTAAAAGGAGCGTTCACA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   4459 GenBank   WP_208557299
Name   mobP2_J4048_RS21755_XJ5|unnamed2 insolico UniProt ID   _
Length   410 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 48348.01 Da        Isoelectric Point: 9.8707

>WP_208557299.1 MULTISPECIES: MobP2 family relaxase [Bacillus]
MDAPGVVLVSKYVSGKSTKFSKYVNYINRDEAVRTEKFQTYNVNKLDGYNQYMGNPEKSSGIFTQFKDTL
SPQEKNQLKEIFRQAQENDSVMWQDVISFDNKWLEEHGIYKPDTGWINEGAIQNSIRKGMKVLLKEEQLE
QSGVWSAAIHYNTDNIHVHIALVEPNPTKEYGVFTNKKTGEVYQARRGNRKLKTLDKMKSKVANTLLDRD
KELAKISQLIHDRIAPKGVKFQPRLDTYMSKMYNHIYANLPEDMRLWRYNNNALNFIRPEIDSVITMYIQ
KYHPEDFKELDQSLKEEMEFRKSVYGDGPIQVERYKEYRENKHKELYAKLGNSMLKEMVEIRKRESQTKQ
FVRTGTYAPGSASQKQWDAKGKLIKRSDINKIKHALDKDYQSMKNMRKYQQMQYEMEQSR

  Protein domains


Predicted by InterproScan.

(4-389)


  Protein structure



No available structure.




T4CP


ID   4506 GenBank   WP_073982219
Name   t4cp2_J4048_RS22095_XJ5|unnamed2 insolico UniProt ID   _
Length   786 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 786 a.a.        Molecular weight: 90647.01 Da        Isoelectric Point: 4.8228

>WP_073982219.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Bacillus]
MHKEFKQWKKIFADKYFLIAFTLFCYAAVTCAANFLLHLFKQIPLMLSSFKTFDQSKLSSPFDSYSWKWF
FEFEWDMGIIYGVLYIIASIFIVRQVYRFRIAFRDINKQTKGTARWTTIKEIQETYKAIKDDDIEYEGSA
GMPIVHFNDHLYVDTNSTHTLVVASTQSGKTETYSYPYLDVIMRAKKKDSVVITDIKGDMLKNTHSEFEK
HGYEVMCFNLLNPYWGMAYNPLELVKQAYMKGDFSKAQMLCNTLSYSLFHNDKSHADPMWENASIALVNA
LILAVCDLCIKNGQPENITMYTLTVMLNELGSNPDEDGYTRLDHFFGNLPPSHPAKLQYGTIQFSQGITR
SGIYTGTMAKLKNYTYDTIARMTAQNDLNIEDLAYGEKPVALFIVYPDWDDSNYTIISTFLSQVNAVLSE
KATLSKESTLPRKVRYLFEEVANIPPIEGLNRALAVGLSRGMLYTLVIQNVSQLRDVYGDDMATAIMGNL
GNQIYIMSDEWEDAEKFSEKLGVTTIISADRQGGLMDVDKSYSEREEERPLMLPDELRRLKKGEWVVLRT
KKRENLKRKRVVPYPIFASLDNETHMLHRYEYLMHRFNNEIALGDLGFHGNHETLDLESLLIEFEFNEPV
EQKNKVKGKKRSDKKDADPEVPDYNEIPPPEEPIGIADESSEAMGSLFELVPSDNTNEEEFYEGPCLIDS
SRNEVLEVSEEDAIMNSLEEVYETSTPISDAIKADQYVYIKFFAQKALTEDEYNYFESLTTIEQLRAFFR
APEKHELYEKIKKHIE

  Protein domains


Predicted by InterproScan.

(147-572)

  Protein structure



No available structure.




Host bacterium


ID   7203 GenBank   NZ_CP071972
Plasmid name   XJ5|unnamed2 Incompatibility group   -
Plasmid size   63621 bp Coordinate of oriT [Strand]   6..296 [+]
Host baterium   Bacillus amyloliquefaciens strain XJ5

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -