Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106726
Name   oriT_pSal-5091_CMY in_silico
Organism   Salmonella enterica subsp. enterica serovar Anatum strain Sal-5091
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP045519 (40720..40808 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pSal-5091_CMY
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4443 GenBank   WP_001360283
Name   nikB_GE195_RS22480_pSal-5091_CMY insolico UniProt ID   A0A637A499
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104011.40 Da        Isoelectric Point: 7.3426

>WP_001360283.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A637A499


Auxiliary protein

ID   1971 GenBank   WP_001283947
Name   WP_001283947_pSal-5091_CMY insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   4474 GenBank   WP_011264051
Name   trbC_GE195_RS22485_pSal-5091_CMY insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86954.10 Da        Isoelectric Point: 6.7713

>WP_011264051.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKNNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 46187..89607

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GE195_RS22485 (GE195_22855) 43903..46194 - 2292 WP_011264051 F-type conjugative transfer protein TrbC -
GE195_RS22490 (GE195_22860) 46187..47257 - 1071 WP_011264050 IncI1-type conjugal transfer protein TrbB trbB
GE195_RS22495 (GE195_22865) 47276..48484 - 1209 WP_011264049 IncI1-type conjugal transfer protein TrbA trbA
GE195_RS22500 (GE195_22870) 48791..49573 - 783 WP_023063753 protein FinQ -
GE195_RS22505 (GE195_22875) 49769..50086 + 318 WP_000118520 quaternary ammonium compound efflux SMR transporter SugE -
GE195_RS22510 (GE195_22880) 50083..50616 - 534 WP_001221666 lipocalin family protein -
GE195_RS22515 (GE195_22885) 50710..51855 - 1146 WP_000976514 extended-spectrum class C beta-lactamase CMY-2 -
GE195_RS22520 (GE195_22890) 52179..53441 - 1263 WP_000608644 IS1380-like element ISEcp1 family transposase -
GE195_RS22525 (GE195_22895) 53538..53969 - 432 WP_243753072 hypothetical protein -
GE195_RS22530 (GE195_22900) 54290..54442 + 153 WP_001387489 Hok/Gef family protein -
GE195_RS22535 (GE195_22905) 54514..54765 - 252 WP_001291964 hypothetical protein -
GE195_RS24205 55266..55361 + 96 WP_000609148 DinQ-like type I toxin DqlB -
GE195_RS22545 55426..55602 - 177 WP_001054897 hypothetical protein -
GE195_RS22550 (GE195_22915) 55994..56203 + 210 WP_000062603 HEAT repeat domain-containing protein -
GE195_RS22555 (GE195_22920) 56275..56937 - 663 WP_000644796 plasmid IncI1-type surface exclusion protein ExcA -
GE195_RS22560 (GE195_22925) 57008..59176 - 2169 WP_023518847 DotA/TraY family protein traY
GE195_RS22565 (GE195_22930) 59273..59857 - 585 WP_001746157 IncI1-type conjugal transfer protein TraX -
GE195_RS22570 (GE195_22935) 59886..61088 - 1203 WP_001189160 IncI1-type conjugal transfer protein TraW traW
GE195_RS22575 (GE195_22940) 61055..61669 - 615 WP_000337399 IncI1-type conjugal transfer protein TraV traV
GE195_RS22580 (GE195_22945) 61669..64713 - 3045 WP_165885054 IncI1-type conjugal transfer protein TraU traU
GE195_RS22585 (GE195_22950) 64803..65603 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
GE195_RS22590 (GE195_22955) 65587..65775 - 189 WP_001277255 putative conjugal transfer protein TraS -
GE195_RS22595 (GE195_22960) 65839..66243 - 405 WP_000086965 IncI1-type conjugal transfer protein TraR traR
GE195_RS22600 (GE195_22965) 66294..66821 - 528 WP_001055569 conjugal transfer protein TraQ traQ
GE195_RS22605 (GE195_22970) 66821..67525 - 705 WP_001493811 IncI1-type conjugal transfer protein TraP traP
GE195_RS22610 (GE195_22975) 67525..68814 - 1290 WP_011264042 conjugal transfer protein TraO traO
GE195_RS22615 (GE195_22980) 68817..69800 - 984 WP_001191880 IncI1-type conjugal transfer protein TraN traN
GE195_RS22620 (GE195_22985) 69811..70503 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
GE195_RS22625 (GE195_22990) 70500..70847 - 348 WP_001055900 conjugal transfer protein traL
GE195_RS22630 (GE195_22995) 70865..74632 - 3768 WP_023518849 LPD7 domain-containing protein -
GE195_RS22635 (GE195_23000) 74722..75273 - 552 WP_000014584 phospholipase D family protein -
GE195_RS22640 (GE195_23005) 75288..75578 - 291 WP_001299214 hypothetical protein traK
GE195_RS22645 (GE195_23010) 75575..76723 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
GE195_RS22650 (GE195_23015) 76720..77538 - 819 WP_000646098 IncI1-type conjugal transfer lipoprotein TraI traI
GE195_RS22655 (GE195_23020) 77535..77993 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
GE195_RS22660 (GE195_23025) 78388..78972 - 585 WP_000977522 histidine phosphatase family protein -
GE195_RS22665 (GE195_23030) 79032..80234 - 1203 WP_000976351 conjugal transfer protein TraF -
GE195_RS22670 (GE195_23035) 80320..81144 - 825 WP_165885055 conjugal transfer protein TraE traE
GE195_RS22675 (GE195_23040) 81295..82449 - 1155 WP_001139957 site-specific integrase -
GE195_RS22680 (GE195_23065) 83779..85101 - 1323 WP_165885056 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
GE195_RS22685 (GE195_23070) 85101..85757 - 657 WP_001193553 prepilin peptidase -
GE195_RS22690 (GE195_23075) 85742..86302 - 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
GE195_RS22695 (GE195_23080) 86312..86926 - 615 WP_000959785 type 4 pilus major pilin -
GE195_RS22700 (GE195_23085) 86944..88041 - 1098 WP_001208805 type II secretion system F family protein -
GE195_RS22705 (GE195_23090) 88054..89607 - 1554 WP_000362202 ATPase, T2SS/T4P/T4SS family virB11
GE195_RS22710 (GE195_23095) 89618..90070 - 453 WP_001247334 type IV pilus biogenesis protein PilP -
GE195_RS22715 (GE195_23100) 90057..91352 - 1296 WP_000752772 type 4b pilus protein PilO2 -
GE195_RS22720 (GE195_23105) 91345..93027 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
GE195_RS22725 (GE195_23110) 93041..93478 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
GE195_RS22730 (GE195_23115) 93478..94545 - 1068 WP_001302629 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   7163 GenBank   NZ_CP045519
Plasmid name   pSal-5091_CMY Incompatibility group   IncI1
Plasmid size   106855 bp Coordinate of oriT [Strand]   40720..40808 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Anatum strain Sal-5091

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrVA2