Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 106707 |
| Name | oriT_FIONA 16800|unnamed1  |
| Organism | Aeromonas caviae strain FIONA 16800 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP121793 (27487..27616 [+], 130 nt) |
| oriT length | 130 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 130 nt
>oriT_FIONA 16800|unnamed1
GTTAACCCGTTTGATCGCGTGATGAATGCGCTAAAAAGTCGAGGCCGTAAGAACGCCCACATTCTGAGCATCTTGCAGCTGGATTGGCCAGCGTCTGAATCGATCATCCATCGCCTGAGCGACTACATCA
GTTAACCCGTTTGATCGCGTGATGAATGCGCTAAAAAGTCGAGGCCGTAAGAACGCCCACATTCTGAGCATCTTGCAGCTGGATTGGCCAGCGTCTGAATCGATCATCCATCGCCTGAGCGACTACATCA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 4431 | GenBank | WP_232048270 |
| Name | mobH_QBS29_RS01310_FIONA 16800|unnamed1 |
UniProt ID | _ |
| Length | 987 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 987 a.a. Molecular weight: 107581.92 Da Isoelectric Point: 4.7871
>WP_232048270.1 MULTISPECIES: MobH family relaxase [Aeromonas]
MDEEDIPRYPPFAKGLPVAPIDKILATQAELIEKVRNSLGFTIEDFNRLVLPVIHRYAAFVHLLPASEAH
HHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPKQRRDNEPRWRLASCFSGLLHDVGKPLADVSITDKN
GVVTWNPYFESLHDWAGRNHVDRYFIRWREKRHKRHEQFSLLAVDRIIPVATREYLSESGPLIIEAMLEA
ISGTSVNQPVTKLMLRADQESVSRDLKQNRLNVDEFSYGVPVERYVFDAIRRLVKTGKWKVNEPGAKVWH
LQQGVFIAWRNLGDLYDLISHDKIPGIPRDPDTLADILIERGFAVPNTVQEKGDRAYYRYWEVLPEMIQE
AAGSIKILMLRLESNELVFTTEPPAAVAGEVVGDVADAVIELIDPDEASDADDESASGMSESLLSAEQEA
MNALAGLGFGDAMEILGGTASDDNATQPAPVVDTTPPAKPAKGAGGRGGKNQAKKTAPAAPEQQQPEPSP
QEIAKSTPPLAVENPLQAIMDVGGGLGGLDFPFDAFVAGTSMSDEPSQALAPEPKQDVANPDNPDTPNLG
LMNQVFPNFMNDESTGDVGGMDLPPAWAIEEIPSFHAYEGEVGQPGNQFVSEELAGADAPDAKGELLTLM
ATFGEAGPILERAILPVLEGKATLGEVLCLMKGQAVILYPEGARALGAPSEILTALFHAQAIVPDPVMSG
RKVQDFNGVKAIVLTEQLSSAFIAAIKQAESLLGGYQDAFELVASPEVKKNQSKKNRGQRKTSRSESSGE
DSHAASPERAQVEEKRVIADAQLIDVSVEPPSPPVPPVDTTPPPDKVVPRKESRGKEKVDKPIQPAPTTK
PAEGTQNLARLPPREQPKPESQQKQARVIDVGNIALPGPDLPEELSPRSIVKEETVDEALYLPPKMTPEK
AIQMLKEMIKNRSGRWLVSAVLEEEGFLTTSDKAFEVIAGEYTGISKHVLGGTLRRAQRRPLLKQRQGKL
YLEVDKS
MDEEDIPRYPPFAKGLPVAPIDKILATQAELIEKVRNSLGFTIEDFNRLVLPVIHRYAAFVHLLPASEAH
HHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPKQRRDNEPRWRLASCFSGLLHDVGKPLADVSITDKN
GVVTWNPYFESLHDWAGRNHVDRYFIRWREKRHKRHEQFSLLAVDRIIPVATREYLSESGPLIIEAMLEA
ISGTSVNQPVTKLMLRADQESVSRDLKQNRLNVDEFSYGVPVERYVFDAIRRLVKTGKWKVNEPGAKVWH
LQQGVFIAWRNLGDLYDLISHDKIPGIPRDPDTLADILIERGFAVPNTVQEKGDRAYYRYWEVLPEMIQE
AAGSIKILMLRLESNELVFTTEPPAAVAGEVVGDVADAVIELIDPDEASDADDESASGMSESLLSAEQEA
MNALAGLGFGDAMEILGGTASDDNATQPAPVVDTTPPAKPAKGAGGRGGKNQAKKTAPAAPEQQQPEPSP
QEIAKSTPPLAVENPLQAIMDVGGGLGGLDFPFDAFVAGTSMSDEPSQALAPEPKQDVANPDNPDTPNLG
LMNQVFPNFMNDESTGDVGGMDLPPAWAIEEIPSFHAYEGEVGQPGNQFVSEELAGADAPDAKGELLTLM
ATFGEAGPILERAILPVLEGKATLGEVLCLMKGQAVILYPEGARALGAPSEILTALFHAQAIVPDPVMSG
RKVQDFNGVKAIVLTEQLSSAFIAAIKQAESLLGGYQDAFELVASPEVKKNQSKKNRGQRKTSRSESSGE
DSHAASPERAQVEEKRVIADAQLIDVSVEPPSPPVPPVDTTPPPDKVVPRKESRGKEKVDKPIQPAPTTK
PAEGTQNLARLPPREQPKPESQQKQARVIDVGNIALPGPDLPEELSPRSIVKEETVDEALYLPPKMTPEK
AIQMLKEMIKNRSGRWLVSAVLEEEGFLTTSDKAFEVIAGEYTGISKHVLGGTLRRAQRRPLLKQRQGKL
YLEVDKS
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 4462 | GenBank | WP_011899407 |
| Name | traD_QBS29_RS01315_FIONA 16800|unnamed1 |
UniProt ID | _ |
| Length | 621 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 621 a.a. Molecular weight: 68931.34 Da Isoelectric Point: 6.8339
>WP_011899407.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTYDPLAYEMPWRPNYEMSAVFGWVAASVGALAVQQITDMPPEPFYWMTGICGVMTMARLPKAIKLHLLQ
KHLKGRDLEFISIKELQKKVQASPEDMWLGYGFAWENRHAQRVFEILKRDWTSIVGTESTTSKIVRTIRG
KKKKEVPIGQPWIHGVEPKEEHLMQPLKHTEGHSLIVGTTGSGKTRMFDTLISQAILRGEAVIIIDPKGD
KEMRDNARRACEAMGQAERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEGGGDPFKSFGWQA
LNNIAQGLILAYDRPNLIKLRRFLEGGADGLVIKAVQAYAERVMPDWEAAAAPYLEKAANGSRSKLAVAH
MAFYNNVIQPEHPSSELEGLLNMFKHDATHFSKMVSNLLPIMNMLTSGELGPLLSPDATDLSDERQITDS
AKIINNAQVAYLGLDSLTDNMVGSALGSIFLSDLTAVAGDRYNYGVNNLPVNIFVDEAAEVINDPFIQLL
NKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNVFALRIVDAETQEYIAENLPKTRLKYVMRTQ
GQNTDGSEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGKIVKGRLPILTEK
MTYDPLAYEMPWRPNYEMSAVFGWVAASVGALAVQQITDMPPEPFYWMTGICGVMTMARLPKAIKLHLLQ
KHLKGRDLEFISIKELQKKVQASPEDMWLGYGFAWENRHAQRVFEILKRDWTSIVGTESTTSKIVRTIRG
KKKKEVPIGQPWIHGVEPKEEHLMQPLKHTEGHSLIVGTTGSGKTRMFDTLISQAILRGEAVIIIDPKGD
KEMRDNARRACEAMGQAERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEGGGDPFKSFGWQA
LNNIAQGLILAYDRPNLIKLRRFLEGGADGLVIKAVQAYAERVMPDWEAAAAPYLEKAANGSRSKLAVAH
MAFYNNVIQPEHPSSELEGLLNMFKHDATHFSKMVSNLLPIMNMLTSGELGPLLSPDATDLSDERQITDS
AKIINNAQVAYLGLDSLTDNMVGSALGSIFLSDLTAVAGDRYNYGVNNLPVNIFVDEAAEVINDPFIQLL
NKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNVFALRIVDAETQEYIAENLPKTRLKYVMRTQ
GQNTDGSEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGKIVKGRLPILTEK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 45999..65640
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| QBS29_RS01405 | 42440..42709 | + | 270 | WP_279473630 | hypothetical protein | - |
| QBS29_RS01410 | 42777..42965 | + | 189 | WP_103859337 | hypothetical protein | - |
| QBS29_RS01415 | 42979..43350 | + | 372 | WP_279473631 | hypothetical protein | - |
| QBS29_RS01420 | 43351..43899 | - | 549 | WP_103859339 | hypothetical protein | - |
| QBS29_RS01425 | 43964..44119 | - | 156 | WP_279473632 | hypothetical protein | - |
| QBS29_RS01430 | 44142..44336 | + | 195 | WP_103859340 | hypothetical protein | - |
| QBS29_RS01435 | 44349..44687 | + | 339 | WP_103859341 | hypothetical protein | - |
| QBS29_RS01440 | 44759..45172 | + | 414 | WP_279473633 | hypothetical protein | - |
| QBS29_RS01445 | 45221..45466 | + | 246 | WP_011899397 | hypothetical protein | - |
| QBS29_RS01450 | 45999..46280 | + | 282 | WP_011899398 | type IV conjugative transfer system protein TraL | traL |
| QBS29_RS01455 | 46277..46912 | + | 636 | WP_073536827 | TraE/TraK family type IV conjugative transfer system protein | traE |
| QBS29_RS01460 | 46896..47801 | + | 906 | WP_103858935 | type-F conjugative transfer system secretin TraK | traK |
| QBS29_RS01465 | 47804..49123 | + | 1320 | WP_088822001 | TraB/VirB10 family protein | traB |
| QBS29_RS01470 | 49120..49686 | + | 567 | WP_073536829 | type IV conjugative transfer system lipoprotein TraV | - |
| QBS29_RS01475 | 49777..50103 | + | 327 | WP_050804919 | TraA family conjugative transfer protein | - |
| QBS29_RS01480 | 50285..55771 | + | 5487 | WP_279473634 | Ig-like domain-containing protein | - |
| QBS29_RS01485 | 55928..56653 | + | 726 | WP_232484092 | DsbC family protein | trbB |
| QBS29_RS01490 | 56650..59093 | + | 2444 | Protein_58 | type IV secretion system protein TraC | - |
| QBS29_RS01495 | 59113..59430 | + | 318 | WP_073536868 | hypothetical protein | - |
| QBS29_RS01500 | 59427..59888 | + | 462 | WP_341536518 | S26 family signal peptidase | - |
| QBS29_RS25695 | 59910..59958 | + | 49 | Protein_61 | hypothetical protein | - |
| QBS29_RS01505 | 60186..61184 | + | 999 | WP_332460100 | TrbC family F-type conjugative pilus assembly protein | - |
| QBS29_RS01510 | 61181..61852 | + | 672 | WP_279473635 | EAL domain-containing protein | - |
| QBS29_RS01515 | 61849..62856 | + | 1008 | WP_073536834 | TraU family protein | traU |
| QBS29_RS01520 | 62866..65640 | + | 2775 | WP_073536835 | conjugal transfer mating pair stabilization protein TraN | traN |
| QBS29_RS01525 | 66705..67568 | - | 864 | WP_073536836 | hypothetical protein | - |
| QBS29_RS01530 | 67713..68381 | - | 669 | WP_081375035 | hypothetical protein | - |
| QBS29_RS01535 | 68670..69032 | + | 363 | WP_073536838 | hypothetical protein | - |
| QBS29_RS01540 | 69324..69503 | + | 180 | WP_073536839 | hypothetical protein | - |
Host bacterium
| ID | 7144 | GenBank | NZ_CP121793 |
| Plasmid name | FIONA 16800|unnamed1 | Incompatibility group | - |
| Plasmid size | 296497 bp | Coordinate of oriT [Strand] | 27487..27616 [+] |
| Host baterium | Aeromonas caviae strain FIONA 16800 |
Cargo genes
| Drug resistance gene | blaIMP-8 |
| Virulence gene | vgrG2, hcp |
| Metal resistance gene | merE, merD, merA, merC, merP, merT, merR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |