Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 106674
• Name: oriT_pRFP2
• Organism: Enterococcus faecalis strain RF_1_1_25_1
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP046249 (2802..2851 [+], 50 nt)
• oriT length: 50 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 50 nt

>oriT_pRFP2
AATATCGCAACATGGTACCATGTTGCTCTGCTCGCAAAAAGAAAGCCTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_GLO19_RS13630_pRFP2

• ID: 4415
• GenBank: WP_243513422
• Name: mobV_GLO19_RS13630_pRFP2
• UniProt ID: _
• Length: 559 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 559 a.a.
• Molecular weight: 65130.53 Da
• Isoelectric Point: 7.9641

>WP_243513422.1 MobV family relaxase [Enterococcus faecalis]
MSKIVARMEKMKDGNLSGIQRHNQRETNNHSNPDIDIEKSHLNYDLVNPGSINYREKIKQIIESQRISKR
AVRKDAVLVNEWIITSDTAFFQENTDTQAFFTDVVAYFSDRCGRQNVAYATVHLDETTPHMHLGIVPMYE
GRLSSKQVFSRQNLLEIQEELPTYLQERGYAIERGLRGSPQKHLSVKEYKDVQKELQVSKQELANQEQIL
AEKERELHSYEQAIFRIDRLLEEKNEEVKGKVQAMNQLETKRNEIINSLQTTVLPDLANVKEKKFSLNGL
KYVLSASDFSAIKSITKDFETLRTQNKHLKIENNNLAQKNKELTQVNHEQELQITELAYELTLKKEQLTA
FEEKQLNYDQSEQIKQELDTTTQTVSVLETENKRLKDRITDLTYDFSELNEKYQEIKLKFDGLTTYLHER
LGHAKETILFTIQQGVKYLQNAPKLKKSLERYSKDRTYLIDEKGTLFLPPDTGSKGVTMSLNPHFYPLKA
PFYNRETGCYEFVGEYMANGRSRTNTLQLSEEQVLEKKAFTFEQSTDKKAWQQSKKYSRDMSKGRGLSR

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (2-191)

Protein structure

No available structure.

Host bacterium

• ID: 7111
• GenBank: NZ_CP046249
• Plasmid name: pRFP2
• Incompatibility group: -
• Plasmid size: 5350 bp
• Coordinate of oriT [Strand]: 2802..2851 [+]
• Host baterium: Enterococcus faecalis strain RF_1_1_25_1

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -