Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106673
Name   oriT_pRFP1 in_silico
Organism   Enterococcus faecalis strain RF_1_1_25_1
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP046248 (49706..49755 [-], 50 nt)
oriT length   50 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_pRFP1
AATATCGCAACATGGTACCATGTTGCTCTGCTCGCAAAAAGAAAGCCTAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4414 GenBank   WP_002367932
Name   Relaxase_GLO19_RS13525_pRFP1 insolico UniProt ID   R3K157
Length   561 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 561 a.a.        Molecular weight: 65400.15 Da        Isoelectric Point: 6.0174

>WP_002367932.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterococcus]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKKVFEQISDKIAAKEGAKIIEKSPKNS
HKKYTMWETESLYKEKIKSRLDFLLEQSSSINDFLEKAEALNLSVDFSKKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIVERLKENKSVLTLKEVVEKYVEKSEKSKNEFDYQLTIDEWQISHKTTRGYYVNVDFG
FGERGKVFIGAYKVDPLENGQYNIYVKRKDFFYLMNEKNADRNRYMTGETLIKQLRLYNGQTPLKKEPVM
RTIDELVNAINFLAANEIEDTRQLELLEEKLEAAFLEAEETLETLDEKMLELHQLSNLLLENELNGELEL
IQQKLKTLLPDATLAEFSYEDVRGEIEAIKTSQSLLENKLERTRNEINQLHEIQAVQKKETANQNNVKPK
L

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(37-296)


  Protein structure


Source ID Structure
AlphaFold DB R3K157


Auxiliary protein

ID   1964 GenBank   WP_002367696
Name   WP_002367696_pRFP1 insolico UniProt ID   Q82YL4
Length   118 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 118 a.a.        Molecular weight: 14042.10 Da        Isoelectric Point: 9.9724

>WP_002367696.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Bacteria]
MENKQKLKRPIQRKIRFSKEENELINRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKEQS

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(64-105)


  Protein structure


Source ID Structure
AlphaFold DB Q82YL4


T4CP

ID   4444 GenBank   WP_010815743
Name   t4cp2_GLO19_RS13545_pRFP1 insolico UniProt ID   _
Length   609 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 609 a.a.        Molecular weight: 70159.16 Da        Isoelectric Point: 6.1803

>WP_010815743.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNDAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(144-558)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 52400..63928

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GLO19_RS13525 (GLO19_13965) 47597..49282 - 1686 WP_002367932 relaxase/mobilization nuclease domain-containing protein -
GLO19_RS13530 (GLO19_13970) 49283..49639 - 357 WP_002367696 plasmid mobilization relaxosome protein MobC -
GLO19_RS13535 (GLO19_13975) 49885..50157 - 273 WP_002362409 LtrD -
GLO19_RS13540 (GLO19_13980) 50190..52349 - 2160 WP_010815744 ArdC-like ssDNA-binding domain-containing protein -
GLO19_RS13545 (GLO19_13985) 52400..54229 - 1830 WP_010815743 VirD4-like conjugal transfer protein, CD1115 family virb4
GLO19_RS13550 (GLO19_13990) 54229..54714 - 486 WP_002386493 PcfB family protein gbs1365
GLO19_RS13555 (GLO19_13995) 54756..54938 - 183 WP_002367689 hypothetical protein -
GLO19_RS13560 (GLO19_14000) 54984..55442 - 459 WP_202522638 single-stranded DNA-binding protein -
GLO19_RS13565 (GLO19_14005) 55459..56214 - 756 WP_002415346 Fic family protein -
GLO19_RS13570 (GLO19_14010) 56328..56936 - 609 WP_010815741 hypothetical protein -
GLO19_RS13575 (GLO19_14015) 56920..57165 - 246 WP_002410289 hypothetical protein -
GLO19_RS13580 (GLO19_14020) 57152..57778 - 627 WP_010815740 hypothetical protein prgL
GLO19_RS13585 (GLO19_14025) 57802..60417 - 2616 WP_172500139 phage tail tip lysozyme prgK
GLO19_RS13590 (GLO19_14030) 60429..62819 - 2391 WP_010815738 VirB4-like conjugal transfer ATPase, CD1110 family virb4
GLO19_RS13595 (GLO19_14035) 62773..63126 - 354 WP_002386485 PrgI family protein prgIc
GLO19_RS13600 (GLO19_14040) 63128..63928 - 801 WP_002382799 type IV secretion system protein prgHb
GLO19_RS13605 (GLO19_14045) 63943..64236 - 294 WP_002406072 hypothetical protein -
GLO19_RS13610 (GLO19_14050) 64249..64476 - 228 WP_002365858 hypothetical protein -
GLO19_RS13615 (GLO19_14055) 64523..64957 - 435 WP_002365857 hypothetical protein -
GLO19_RS13620 (GLO19_14060) 64977..65876 - 900 WP_002409287 hypothetical protein -

Host bacterium

ID   7110 GenBank   NZ_CP046248
Plasmid name   pRFP1 Incompatibility group   -
Plasmid size   66565 bp Coordinate of oriT [Strand]   49706..49755 [-]
Host baterium   Enterococcus faecalis strain RF_1_1_25_1

Cargo genes

Drug resistance gene   -
Virulence gene   prgB/asc10
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA9