Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 106666 |
| Name | oriT_SCB469|unnamed1  |
| Organism | Lactococcus lactis strain SCB469 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP094475 (22821..22858 [+], 38 nt) |
| oriT length | 38 nt |
| IRs (inverted repeats) | 1..7, 19..25 (ACACCAC..GTGGTGT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 38 nt
>oriT_SCB469|unnamed1
ACACCACCCAATTTTGGAGTGGTGTGTAAGTGCGCATT
ACACCACCCAATTTTGGAGTGGTGTGTAAGTGCGCATT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 4411 | GenBank | WP_010890626 |
| Name | mobQ_MTO79_RS12760_SCB469|unnamed1 |
UniProt ID | A0A8B3ETK9 |
| Length | 680 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 680 a.a. Molecular weight: 79275.45 Da Isoelectric Point: 6.7052
>WP_010890626.1 MULTISPECIES: MobQ family relaxase [Lactococcus]
MAIFHMNFSNISAGKGRSAVASASYRSGEKLYSEMENKTYFYNRSVMPESFILLPENAPEWAKDRQKLWN
EVEAVDRKVNSRYAKEFNVALPIELSEDEQKELLTEYVQKIFVDKGMVADVAIHRDHDENPHAHVMLTNR
PFNADGSWGQKAKKEYILDENGNKTYTANGHARSRKIWLVDWDKVGKVEEWRKAWADHVNSVFQEKNIDE
RISEKTLEAQGINDIATQHVGVTGNRDERAEFNKLVLENRQHKAELENLDEKINNELKVKQLKNFYSFNE
KKVIAELSKELHTFIDLEHLEEKNKMLFNWKNSVLIKQIVGKDVSEELNKVSRQEFSLDNANRLIDKVVD
RSIKAMYPTVDSNAFSMPEKRQLIRETESENKVFTGAELDDRLSMIRADSLNRQIVALTKRPFTSLTMLS
KQEKYHIDNINNVLAYQGYNYENIEMSHGKILRNYESEEFSKELDIIQRSLKQINSIEEVRKIVEQQYSV
VLGTIFPDSELNKLSLVEKEKTYNAVIYFNPTIKKLTQSELENIVKEPPILFSTKEHNQGLSYLAGRIRL
EDINNKHLTRVLKFEGTKKLFIGEAKADKNIDPELLKEATNRNDKQSYKNEFYRDKNMENYQSVAYSDTS
PAEYMNRLFSGVLISVLYNNENQHKKGLEEVEWDMERKHREHTKTGGLSR
MAIFHMNFSNISAGKGRSAVASASYRSGEKLYSEMENKTYFYNRSVMPESFILLPENAPEWAKDRQKLWN
EVEAVDRKVNSRYAKEFNVALPIELSEDEQKELLTEYVQKIFVDKGMVADVAIHRDHDENPHAHVMLTNR
PFNADGSWGQKAKKEYILDENGNKTYTANGHARSRKIWLVDWDKVGKVEEWRKAWADHVNSVFQEKNIDE
RISEKTLEAQGINDIATQHVGVTGNRDERAEFNKLVLENRQHKAELENLDEKINNELKVKQLKNFYSFNE
KKVIAELSKELHTFIDLEHLEEKNKMLFNWKNSVLIKQIVGKDVSEELNKVSRQEFSLDNANRLIDKVVD
RSIKAMYPTVDSNAFSMPEKRQLIRETESENKVFTGAELDDRLSMIRADSLNRQIVALTKRPFTSLTMLS
KQEKYHIDNINNVLAYQGYNYENIEMSHGKILRNYESEEFSKELDIIQRSLKQINSIEEVRKIVEQQYSV
VLGTIFPDSELNKLSLVEKEKTYNAVIYFNPTIKKLTQSELENIVKEPPILFSTKEHNQGLSYLAGRIRL
EDINNKHLTRVLKFEGTKKLFIGEAKADKNIDPELLKEATNRNDKQSYKNEFYRDKNMENYQSVAYSDTS
PAEYMNRLFSGVLISVLYNNENQHKKGLEEVEWDMERKHREHTKTGGLSR
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A8B3ETK9 |
T4CP
| ID | 4441 | GenBank | WP_010890635 |
| Name | t4cp2_MTO79_RS12700_SCB469|unnamed1 |
UniProt ID | _ |
| Length | 530 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 530 a.a. Molecular weight: 59615.30 Da Isoelectric Point: 4.8001
>WP_010890635.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Lactococcus]
MNGTILGVLDNKIIYQDNTTKPNRNVMVIGGSGSYKTQSVVITNLFNETKNSIVVTDPKGELYEKTAGIK
LAQGYEVHVVNFANMAHSDRYNPFDYIERDIQAESVATKIVQSENAEGKKDVWFSTQRQLLKALILFVMK
ERSPEQRNLAGVINVLQTFDSEPINKDENSDLDNLFLALKITHPARIAYELGFKKAKGDMKASIISSLLA
TISKFTDEEVSNFTSISDFHLQDIGRKKIVLYVIIPVMDNTYESFINLFFSQMFDELYKLASSNGAKLPQ
EVDFILDEFVNLGKFPKYEEFLATCRGYGIGVTTICQTLTQLQSLYGKEKAESILGNHAVKICLNASNEA
TAKYFSELLGKSTVKVETGSESTSHSKETSTSKSDSYSYTSRQLMTPDEIIRMPDTQSLLIFTNQKPIKA
TKAFQFKLFPDADSKVKLEQNKYVGITSKSQLEKYNDLSVKWEEKLQSLKNITVTEEEEKDLQDNIDSDL
EAMNNENSGVDNSTTETDKIAETEAKEVQAEEKNFDQVVL
MNGTILGVLDNKIIYQDNTTKPNRNVMVIGGSGSYKTQSVVITNLFNETKNSIVVTDPKGELYEKTAGIK
LAQGYEVHVVNFANMAHSDRYNPFDYIERDIQAESVATKIVQSENAEGKKDVWFSTQRQLLKALILFVMK
ERSPEQRNLAGVINVLQTFDSEPINKDENSDLDNLFLALKITHPARIAYELGFKKAKGDMKASIISSLLA
TISKFTDEEVSNFTSISDFHLQDIGRKKIVLYVIIPVMDNTYESFINLFFSQMFDELYKLASSNGAKLPQ
EVDFILDEFVNLGKFPKYEEFLATCRGYGIGVTTICQTLTQLQSLYGKEKAESILGNHAVKICLNASNEA
TAKYFSELLGKSTVKVETGSESTSHSKETSTSKSDSYSYTSRQLMTPDEIIRMPDTQSLLIFTNQKPIKA
TKAFQFKLFPDADSKVKLEQNKYVGITSKSQLEKYNDLSVKWEEKLQSLKNITVTEEEEKDLQDNIDSDL
EAMNNENSGVDNSTTETDKIAETEAKEVQAEEKNFDQVVL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 9401..27634
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MTO79_RS12670 (MTO79_12670) | 5426..5878 | + | 453 | WP_058147845 | hypothetical protein | - |
| MTO79_RS12675 (MTO79_12675) | 5926..6993 | - | 1068 | WP_058147844 | ArdC family protein | - |
| MTO79_RS12680 (MTO79_12680) | 6996..7205 | - | 210 | WP_010890684 | hypothetical protein | - |
| MTO79_RS12685 (MTO79_12685) | 7208..9385 | - | 2178 | WP_058147843 | type IA DNA topoisomerase | - |
| MTO79_RS12690 (MTO79_12690) | 9401..10273 | - | 873 | WP_081039421 | conjugal transfer protein TrbL family protein | trsL |
| MTO79_RS12695 (MTO79_12695) | 10277..10651 | - | 375 | WP_058147842 | hypothetical protein | - |
| MTO79_RS12700 (MTO79_12700) | 10666..12258 | - | 1593 | WP_010890635 | VirD4-like conjugal transfer protein, CD1115 family | - |
| MTO79_RS12705 (MTO79_12705) | 12255..12713 | - | 459 | WP_010890634 | hypothetical protein | trsJ |
| MTO79_RS12710 (MTO79_12710) | 12710..13075 | - | 366 | WP_223203862 | thioredoxin | - |
| MTO79_RS12715 (MTO79_12715) | 13068..13685 | - | 618 | WP_010890664 | hypothetical protein | - |
| MTO79_RS12720 (MTO79_12720) | 13698..14855 | - | 1158 | WP_010890632 | phage tail tip lysozyme | prgK |
| MTO79_RS12725 (MTO79_12725) | 14857..16275 | - | 1419 | WP_058147841 | type VII secretion protein EssB/YukC | - |
| MTO79_RS12730 (MTO79_12730) | 16268..18286 | - | 2019 | WP_010890630 | DUF87 domain-containing protein | virb4 |
| MTO79_RS12735 (MTO79_12735) | 18298..18951 | - | 654 | WP_010890629 | TrsD/TraD family conjugative transfer protein | trsD |
| MTO79_RS12740 (MTO79_12740) | 18935..19282 | - | 348 | WP_010890628 | hypothetical protein | trsC |
| MTO79_RS12745 (MTO79_12745) | 19303..19647 | - | 345 | WP_058147840 | CagC family type IV secretion system protein | - |
| MTO79_RS12750 (MTO79_12750) | 19660..20265 | - | 606 | WP_010890663 | hypothetical protein | - |
| MTO79_RS12755 (MTO79_12755) | 20266..20610 | - | 345 | WP_010890662 | hypothetical protein | - |
| MTO79_RS12760 (MTO79_12760) | 20696..22738 | - | 2043 | WP_010890626 | MobQ family relaxase | - |
| MTO79_RS12765 (MTO79_12765) | 23007..23291 | + | 285 | WP_058147839 | hypothetical protein | - |
| MTO79_RS12770 (MTO79_12770) | 23304..23570 | + | 267 | WP_010890660 | hypothetical protein | - |
| MTO79_RS12775 (MTO79_12775) | 23853..24053 | - | 201 | WP_058147838 | hypothetical protein | - |
| MTO79_RS12780 (MTO79_12780) | 24068..24385 | - | 318 | WP_058147852 | hypothetical protein | - |
| MTO79_RS12785 (MTO79_12785) | 24864..26018 | - | 1155 | WP_010890657 | RepB family plasmid replication initiator protein | - |
| MTO79_RS12790 (MTO79_12790) | 26690..27634 | - | 945 | WP_063283863 | toprim domain-containing protein | traP |
| MTO79_RS12795 (MTO79_12795) | 27660..28040 | - | 381 | WP_010890681 | single-stranded DNA-binding protein | - |
| MTO79_RS12800 (MTO79_12800) | 28439..29197 | - | 759 | WP_003331414 | IS21-like element IS712 family helper ATPase IstB | - |
| MTO79_RS12805 (MTO79_12805) | 29209..30432 | - | 1224 | WP_003331415 | IS21-like element IS712 family transposase | - |
| MTO79_RS12810 (MTO79_12810) | 30682..30948 | - | 267 | WP_010890679 | hypothetical protein | - |
| MTO79_RS12815 (MTO79_12815) | 30962..31759 | - | 798 | WP_015081778 | AAA family ATPase | - |
| MTO79_RS12820 (MTO79_12820) | 31903..32460 | - | 558 | WP_015081779 | recombinase family protein | - |
Host bacterium
| ID | 7103 | GenBank | NZ_CP094475 |
| Plasmid name | SCB469|unnamed1 | Incompatibility group | - |
| Plasmid size | 75968 bp | Coordinate of oriT [Strand] | 22821..22858 [+] |
| Host baterium | Lactococcus lactis strain SCB469 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |