Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106662
Name   oriT_p5_CS00014 in_silico
Organism   Morganella morganii strain CS00014
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JASISH010000007 (45127..45232 [-], 106 nt)
oriT length   106 nt
IRs (inverted repeats)      30..35, 41..46  (CCGCCG..CGGCGG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 106 nt

>oriT_p5_CS00014
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4409 GenBank   WP_004187323
Name   Relaxase_QM172_RS20415_p5_CS00014 insolico UniProt ID   A0A3U8KUL3
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75179.89 Da        Isoelectric Point: 9.9948

>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(86-334)


  Protein structure


Source ID Structure
AlphaFold DB A0A3U8KUL3


T4CP

ID   4439 GenBank   WP_004206885
Name   t4cp2_QM172_RS20295_p5_CS00014 insolico UniProt ID   _
Length   695 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 695 a.a.        Molecular weight: 79538.91 Da        Isoelectric Point: 4.8317

>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 15057..42331

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QM172_RS20255 10661..11458 - 798 WP_015059991 OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-48 -
QM172_RS20260 11677..12374 + 698 WP_095033700 IS1-like element IS1B family transposase -
QM172_RS20265 12371..13576 + 1206 WP_025987686 IS4-like element IS10A family transposase -
QM172_RS20270 13591..14025 - 435 Protein_17 CPBP family intramembrane glutamate endopeptidase -
QM172_RS20275 14138..14488 + 351 WP_124065455 DUF1496 domain-containing protein -
QM172_RS20280 14593..14988 + 396 WP_004187436 lytic transglycosylase domain-containing protein -
QM172_RS20285 15057..16295 + 1239 WP_225373168 conjugal transfer protein TrbA trbA
QM172_RS20290 16306..17256 + 951 WP_004206886 DsbC family protein trbB
QM172_RS20295 17269..19356 + 2088 WP_004206885 conjugal transfer protein TrbC -
QM172_RS20300 19407..19502 - 96 WP_004206884 DinQ-like type I toxin DqlB -
QM172_RS20305 20725..21792 - 1068 WP_019725043 plasmid replication initiator RepA -
QM172_RS20310 22076..22306 - 231 WP_011091085 IncL/M type plasmid replication protein RepC -
QM172_RS20315 22387..23040 - 654 WP_015060005 hypothetical protein -
QM172_RS20320 23042..25237 - 2196 WP_015062834 DotA/TraY family protein traY
QM172_RS20325 25230..25826 - 597 WP_015060003 hypothetical protein -
QM172_RS20330 25823..27031 - 1209 WP_011091082 conjugal transfer protein TraW traW
QM172_RS20335 27028..30078 - 3051 WP_004187482 hypothetical protein traU
QM172_RS20340 30075..30569 - 495 WP_004187480 hypothetical protein -
QM172_RS20345 30615..31004 - 390 WP_004187479 DUF6750 family protein traR
QM172_RS20350 31021..31551 - 531 WP_004187478 conjugal transfer protein TraQ traQ
QM172_RS20355 31575..32279 - 705 WP_015060002 conjugal transfer protein TraP traP
QM172_RS20360 32291..33640 - 1350 WP_004187474 conjugal transfer protein TraO traO
QM172_RS20365 33652..34803 - 1152 WP_004187471 DotH/IcmK family type IV secretion protein traN
QM172_RS20370 34812..35525 - 714 WP_004187467 DotI/IcmL family type IV secretion protein traM
QM172_RS20375 35593..36003 + 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
QM172_RS20380 36032..36187 + 156 WP_172693602 Hha/YmoA family nucleoid-associated regulatory protein -
QM172_RS20385 36188..36700 - 513 WP_011091071 hypothetical protein traL
QM172_RS20390 36666..40103 - 3438 WP_015586048 LPD7 domain-containing protein -
QM172_RS20395 40128..40388 - 261 WP_004187310 IcmT/TraK family protein traK
QM172_RS20400 40378..41541 - 1164 WP_004187313 plasmid transfer ATPase TraJ virB11
QM172_RS20405 41552..42331 - 780 WP_004187315 type IV secretory system conjugative DNA transfer family protein traI
QM172_RS20410 42328..42828 - 501 WP_004187320 DotD/TraH family lipoprotein -
QM172_RS20415 42842..44821 - 1980 WP_004187323 TraI/MobA(P) family conjugative relaxase -
QM172_RS20420 44808..45335 - 528 WP_172740549 plasmid mobilization protein MobA -
QM172_RS20425 45370..45765 + 396 WP_019725163 hypothetical protein -
QM172_RS20430 46267..46803 - 537 WP_004187332 hypothetical protein -
QM172_RS20435 46936..47247 - 312 WP_004187333 hypothetical protein -

Host bacterium

ID   7099 GenBank   NZ_JASISH010000007
Plasmid name   p5_CS00014 Incompatibility group   IncL/M
Plasmid size   65489 bp Coordinate of oriT [Strand]   45127..45232 [-]
Host baterium   Morganella morganii strain CS00014

Cargo genes

Drug resistance gene   blaOXA-48
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -