Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 106649 |
Name | oriT_RHB35-E2-C08|unnamed2 |
Organism | Escherichia marmotae strain RHB35-E2-C08 |
Sequence Completeness | - |
NCBI accession of oriT (coordinates [strand]) | NZ_CP099346 (6357..6417 [-], 61 nt) |
oriT length | 61 nt |
IRs (inverted repeats) | _ |
Location of nic site | _ |
Conserved sequence flanking the nic site |
_ |
Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 61 nt
>oriT_RHB35-E2-C08|unnamed2
GGGTTTCGGAGCGCAGCCCTGAACCAGTTCACAGAGCGCTAGCGGAGTGTATACCGGCTTA
GGGTTTCGGAGCGCAGCCCTGAACCAGTTCACAGAGCGCTAGCGGAGTGTATACCGGCTTA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileT4CP
ID | 4431 | GenBank | WP_279283667 |
Name | traC_NFK54_RS24515_RHB35-E2-C08|unnamed2 | UniProt ID | _ |
Length | 879 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 879 a.a. Molecular weight: 100388.22 Da Isoelectric Point: 6.4549
>WP_279283667.1 type IV secretion system protein TraC [Escherichia marmotae]
MSRERQPEQQEVKGWQVLLSQLLGGWKTPDGSQQAQQILDEMDYPSFASLLPYRRYDPDSELFINARTAG
FILECAPLSGANEAISKSLDYFLRDKIPRKVPLTFMLVGSPCVRPMLERGLADFRWQGKRADEFNAITRA
YYERAALTKFANKKGYPLTLRHYRLFVSYAEEVSSLTEQKKTELNQVANRVVAGLASAGLWSTRVDKHGL
VSLVRELANFRHGNTEPPGGDVAEFEPLNTECIDRSLRLMLHPDRIEQSLQSQRGGERERTRILNFMLEK
NPEKPYALWQMGDNISNVTDPLCTISCPFVMTLALEVEEQAGTQREANRKFIQLDKKANSPFAKFVPSVK
KQAAEWGGLRESLSTNQDSLARFYFNVTLFCEDRDEVALETEMTVLNTFRRNGLSMYMPEFMQLRNYLSV
FPFMMPEGLWSDICRSNAIQRARASNVANILPVVADNQVCLQGLPIPSYRNQLSFLNLFDRNAGLDTDNN
NLSVTGTSGGGKSFLMQGIIRQVLDSGGRAWVFDMGDSYKGLCRNVGGVYLNALDLRFNPFANIKDITAS
AESIRNLLVVLANPSGTCDDTFMSLLLKAVHEVWLRKKNSARIDDIVLYMREALEQPEYRDTTTVRSRMD
EIIVGLEKYCSWGIYGDYFNSEKPSLDDNVRFSVLEMGELKDKPDLLAAVMFSMMIYVEQRMYLSDRQQH
KVCLIDEAWKLLSKDNKRAEDFIENGYRTARKYNGAYITITQGIEDFDGQKASGAAKAAWANSSFKIILR
QNRDAFRKYCLDNPDQFNRFEREVIEGFPAAREAHFSAFMLRYGGQVSFHRLLLDPISRVMFSSDGEDFD
YREAGLRAGKDIHDIIGELAWRKFPQEMETLTEWIKHNH
MSRERQPEQQEVKGWQVLLSQLLGGWKTPDGSQQAQQILDEMDYPSFASLLPYRRYDPDSELFINARTAG
FILECAPLSGANEAISKSLDYFLRDKIPRKVPLTFMLVGSPCVRPMLERGLADFRWQGKRADEFNAITRA
YYERAALTKFANKKGYPLTLRHYRLFVSYAEEVSSLTEQKKTELNQVANRVVAGLASAGLWSTRVDKHGL
VSLVRELANFRHGNTEPPGGDVAEFEPLNTECIDRSLRLMLHPDRIEQSLQSQRGGERERTRILNFMLEK
NPEKPYALWQMGDNISNVTDPLCTISCPFVMTLALEVEEQAGTQREANRKFIQLDKKANSPFAKFVPSVK
KQAAEWGGLRESLSTNQDSLARFYFNVTLFCEDRDEVALETEMTVLNTFRRNGLSMYMPEFMQLRNYLSV
FPFMMPEGLWSDICRSNAIQRARASNVANILPVVADNQVCLQGLPIPSYRNQLSFLNLFDRNAGLDTDNN
NLSVTGTSGGGKSFLMQGIIRQVLDSGGRAWVFDMGDSYKGLCRNVGGVYLNALDLRFNPFANIKDITAS
AESIRNLLVVLANPSGTCDDTFMSLLLKAVHEVWLRKKNSARIDDIVLYMREALEQPEYRDTTTVRSRMD
EIIVGLEKYCSWGIYGDYFNSEKPSLDDNVRFSVLEMGELKDKPDLLAAVMFSMMIYVEQRMYLSDRQQH
KVCLIDEAWKLLSKDNKRAEDFIENGYRTARKYNGAYITITQGIEDFDGQKASGAAKAAWANSSFKIILR
QNRDAFRKYCLDNPDQFNRFEREVIEGFPAAREAHFSAFMLRYGGQVSFHRLLLDPISRVMFSSDGEDFD
YREAGLRAGKDIHDIIGELAWRKFPQEMETLTEWIKHNH
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
ID | 4432 | GenBank | WP_000099426 |
Name | traD_NFK54_RS24620_RHB35-E2-C08|unnamed2 | UniProt ID | _ |
Length | 728 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 728 a.a. Molecular weight: 83452.80 Da Isoelectric Point: 5.0806
>WP_000099426.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Escherichia]
MSSTKHITQGGQVFSYMLNMFMQVNKRVSFWLIWFFVIFLPLFFWLRLPWETIRNGGLYWWLSLTARGEK
ALYRVPPVYDIPWNGQVLHATSEQILKDDYMVWAGNMFLQELYLALFWASVAVGVLAFLIFRFLRRLGEK
QAQDERIGGRELTDDVKAVAREMRRRGEASSICIDKLPLLLNSEVQYLMMYGTPGAGKSNTLNKLLKQIR
ARGDMAIIYDKGCSLIKKHFSEQDDVLLNALDRRCAYWDMFREFESIPDFDSAASTLIPMGTKEDPFWQS
SARTIFSAVAYRQKKNGIHSYNALLRTLLAIDLKALRDYLAGTEASNLVEEKVEKTAISIRSVLTNYVKA
LRYLQGIERTGRRPFTIREWMSTVNDPQITRHGWLWVTSNARQHESLKPLISMWLAQAANCLLGMGENQH
RRIWFIYDELPSLNKLPELPGVLAEARKFGGCFVLGFQAKAQMDYTYGKEFADAMLGLVNTRYFFRSPSS
TEAEWVQREIGQRRDKVFSEQYSYGADTVRDGVSFSKVEEDRYLVNYTDIQKLPNLHCYVTFPGEYPAVR
MRMSYEKIKDCAEELLLRDINDSLDPEIESEITRREDEDGDIAALLDRIAQGDVRKDDEQNKTASQAGQA
ESLNNPVLAAAVTQGTAAAMTGGTGESNPVEEKAPVAELTEIVDKETGEILYPDDERYDELCRDSGQAQE
AMRQDECNLVSHQTNEAREQDDDREVNW
MSSTKHITQGGQVFSYMLNMFMQVNKRVSFWLIWFFVIFLPLFFWLRLPWETIRNGGLYWWLSLTARGEK
ALYRVPPVYDIPWNGQVLHATSEQILKDDYMVWAGNMFLQELYLALFWASVAVGVLAFLIFRFLRRLGEK
QAQDERIGGRELTDDVKAVAREMRRRGEASSICIDKLPLLLNSEVQYLMMYGTPGAGKSNTLNKLLKQIR
ARGDMAIIYDKGCSLIKKHFSEQDDVLLNALDRRCAYWDMFREFESIPDFDSAASTLIPMGTKEDPFWQS
SARTIFSAVAYRQKKNGIHSYNALLRTLLAIDLKALRDYLAGTEASNLVEEKVEKTAISIRSVLTNYVKA
LRYLQGIERTGRRPFTIREWMSTVNDPQITRHGWLWVTSNARQHESLKPLISMWLAQAANCLLGMGENQH
RRIWFIYDELPSLNKLPELPGVLAEARKFGGCFVLGFQAKAQMDYTYGKEFADAMLGLVNTRYFFRSPSS
TEAEWVQREIGQRRDKVFSEQYSYGADTVRDGVSFSKVEEDRYLVNYTDIQKLPNLHCYVTFPGEYPAVR
MRMSYEKIKDCAEELLLRDINDSLDPEIESEITRREDEDGDIAALLDRIAQGDVRKDDEQNKTASQAGQA
ESLNNPVLAAAVTQGTAAAMTGGTGESNPVEEKAPVAELTEIVDKETGEILYPDDERYDELCRDSGQAQE
AMRQDECNLVSHQTNEAREQDDDREVNW
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 35566..63187
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
NFK54_RS24410 (NFK54_24315) | 31333..31482 | + | 150 | Protein_39 | plasmid maintenance protein Mok | - |
NFK54_RS24415 (NFK54_24320) | 31424..31549 | + | 126 | WP_001372321 | type I toxin-antitoxin system Hok family toxin | - |
NFK54_RS24420 (NFK54_24325) | 31850..32146 | - | 297 | Protein_41 | hypothetical protein | - |
NFK54_RS24425 (NFK54_24330) | 32216..32422 | + | 207 | WP_127766627 | single-stranded DNA-binding protein | - |
NFK54_RS24430 (NFK54_24335) | 32446..32742 | + | 297 | WP_032083169 | hypothetical protein | - |
NFK54_RS24435 (NFK54_24340) | 32901..33143 | + | 243 | WP_000540591 | hypothetical protein | - |
NFK54_RS24440 (NFK54_24345) | 33140..33259 | + | 120 | Protein_45 | class I SAM-dependent methyltransferase | - |
NFK54_RS24445 (NFK54_24350) | 33348..33764 | - | 417 | Protein_46 | hypothetical protein | - |
NFK54_RS24450 (NFK54_24355) | 33834..34040 | + | 207 | WP_279283664 | single-stranded DNA-binding protein | - |
NFK54_RS24455 (NFK54_24360) | 34063..34359 | + | 297 | WP_001272240 | hypothetical protein | - |
NFK54_RS24460 (NFK54_24365) | 34466..35287 | + | 822 | WP_001234451 | DUF932 domain-containing protein | - |
NFK54_RS24465 (NFK54_24370) | 35566..36018 | - | 453 | WP_024212709 | transglycosylase SLT domain-containing protein | virB1 |
NFK54_RS24470 (NFK54_24375) | 36531..36926 | + | 396 | WP_001137965 | conjugal transfer relaxosome DNA-binding protein TraM | - |
NFK54_RS24475 (NFK54_24380) | 37088..37816 | + | 729 | WP_135403573 | hypothetical protein | - |
NFK54_RS24480 (NFK54_24385) | 37901..38107 | + | 207 | WP_135403572 | TraY domain-containing protein | - |
NFK54_RS24485 (NFK54_24390) | 38409..38792 | + | 384 | WP_279283665 | type IV conjugative transfer system pilin TraA | - |
NFK54_RS24490 (NFK54_24395) | 38823..39122 | + | 300 | WP_001204091 | type IV conjugative transfer system protein TraL | traL |
NFK54_RS24495 (NFK54_24400) | 39133..39711 | + | 579 | WP_000365869 | type IV conjugative transfer system protein TraE | traE |
NFK54_RS24500 (NFK54_24405) | 39686..40465 | + | 780 | WP_279283666 | type-F conjugative transfer system secretin TraK | traK |
NFK54_RS24505 (NFK54_24410) | 40458..41792 | + | 1335 | WP_053272771 | F-type conjugal transfer pilus assembly protein TraB | traB |
NFK54_RS24510 (NFK54_24415) | 41804..42316 | + | 513 | WP_001558300 | type IV conjugative transfer system lipoprotein TraV | traV |
NFK54_RS24515 (NFK54_24420) | 42313..44952 | + | 2640 | WP_279283667 | type IV secretion system protein TraC | virb4 |
NFK54_RS24520 (NFK54_24425) | 44931..45347 | + | 417 | WP_279283668 | type-F conjugative transfer system protein TrbI | - |
NFK54_RS24525 (NFK54_24430) | 45350..45574 | + | 225 | WP_000752802 | TraR/DksA C4-type zinc finger protein | - |
NFK54_RS24530 (NFK54_24435) | 45571..46188 | + | 618 | WP_167780306 | type-F conjugative transfer system protein TraW | traW |
NFK54_RS24535 (NFK54_24440) | 46185..46580 | + | 396 | WP_151567967 | hypothetical protein | - |
NFK54_RS24540 (NFK54_24445) | 46577..47575 | + | 999 | WP_001558295 | conjugal transfer pilus assembly protein TraU | traU |
NFK54_RS24545 (NFK54_24450) | 47584..48069 | + | 486 | WP_000216829 | hypothetical protein | - |
NFK54_RS24550 (NFK54_24455) | 48251..48499 | + | 249 | Protein_67 | conjugal transfer protein TraP | - |
NFK54_RS24555 (NFK54_24460) | 48486..48803 | + | 318 | Protein_68 | conjugal transfer protein TrbD | - |
NFK54_RS24560 (NFK54_24465) | 48971..49576 | + | 606 | WP_250130495 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
NFK54_RS24565 (NFK54_24470) | 49573..51507 | + | 1935 | WP_279283669 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
NFK54_RS24570 (NFK54_24475) | 51509..52294 | + | 786 | WP_001247978 | type-F conjugative transfer system pilin assembly protein TraF | traF |
NFK54_RS24575 (NFK54_24480) | 52312..52584 | + | 273 | WP_001558287 | hypothetical protein | - |
NFK54_RS24580 (NFK54_24485) | 52586..53161 | + | 576 | WP_000265824 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
NFK54_RS24585 (NFK54_24490) | 53158..53547 | + | 390 | WP_001202475 | hypothetical protein | - |
NFK54_RS24590 (NFK54_24495) | 53613..55019 | + | 1407 | WP_001558285 | conjugal transfer pilus assembly protein TraH | traH |
NFK54_RS24595 (NFK54_24500) | 55019..57895 | + | 2877 | WP_279283693 | conjugal transfer mating-pair stabilization protein TraG | traG |
NFK54_RS24600 (NFK54_24505) | 57910..58821 | + | 912 | WP_001558283 | hypothetical protein | - |
NFK54_RS24605 (NFK54_24510) | 58893..59630 | + | 738 | WP_000926075 | conjugal transfer complement resistance protein TraT | - |
NFK54_RS24610 (NFK54_24515) | 59758..60450 | + | 693 | WP_000235175 | hypothetical protein | - |
NFK54_RS24615 (NFK54_24520) | 60592..60960 | + | 369 | WP_237708211 | DUF2726 domain-containing protein | - |
NFK54_RS24620 (NFK54_24525) | 61001..63187 | + | 2187 | WP_000099426 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
ID | 7086 | GenBank | NZ_CP099346 |
Plasmid name | RHB35-E2-C08|unnamed2 | Incompatibility group | IncFII |
Plasmid size | 72595 bp | Coordinate of oriT [Strand] | 6357..6417 [-] |
Host baterium | Escherichia marmotae strain RHB35-E2-C08 |
Cargo genes
Drug resistance gene | - |
Virulence gene | - |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | - |