Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106621
Name   oriT_pLAP2_050004 in_silico
Organism   Enterobacter chengduensis strain WCHECl-C4 = WCHECh050004
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP043319 (55910..55996 [-], 87 nt)
oriT length   87 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 87 nt

>oriT_pLAP2_050004
GGGTGTCGGGGCGGAGCCCTGACCAGGTGGCATTTGTGATATCGTGCGTGCGCGGTATTACAAATGCACATCCTGTCCCGTCTTTCG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4390 GenBank   WP_022650008
Name   Relaxase_FY206_RS25000_pLAP2_050004 insolico UniProt ID   A0A4Q2QTK9
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 102314.37 Da        Isoelectric Point: 8.2756

>WP_022650008.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacterales]
MIPVIPPKRQDGKSSFGDLVSYVSVRDEPRDDDLMEAVRASGTKPEMPHSSRFSRLVDYATKLRDESFIS
LVDVMPDGGEWVNFYGVTCFHNCTSIESAAEEMEFSARKTKFAHNKSDPVFHYILSWQSHESPRPEQIYD
SVSHTLSRLGLAGHQFVAAVHTDTDNLHVHIAVNRVHPETGYLNRLAYSQEKLSKACRELELKHGFSPDN
GCYVIAPDNRIVRRTSVERDRQSAWKRGRNQSLREYIADTAIAGLRESPVTDWSSLHQRFAKEGLFLTKE
SGELKVKDGWDSERPGVTLSAFGHAFDTDKLIRKLGEFVPPVQDIFSQVPAVGRYEPDKIIMPSRPERMT
EKESLSDYAIARLRAPLIALDQDPTQRTIQSVHTLLAQSGLYLKEQHDRLVICDGYDSTRTPVRAERVWP
ALTKAILDSYKGGWQPVPKDIFQQVPPAEQFTGGSLEATPVSDREWRKLRTGSGPQGALKREIFSDKESL
WGYAVSHCRQDIESLIGSGEFTWERCHELLARQGLLLMREQQGLVVMDAYNHEQTPVKASHVHPDLTLAR
AEPHAGSFVSVPADIFERVKPASRYNPELAVSDRDIPGMKRDPELRRQRREARAAAREDLKARYAAWRAN
WQRPDLHGRERYQAIHDECRIRKARIRIEHRDPLVRKLHYHIAELQRMQALIALKETMKAERAELIGQGK
WYPPSYRQWVEAEAVKGDKAAISQLRGWDYRDRRGNAVKSTTDNRCVVICEPGGTPMYSGVPGLRASLKK
NGRVQFRDPETGRHICTDYGDRVIFRNSGDYDALKRDMVKVAPVLFSRSPSVAMAPEGNNEQFNTAFAKM
VAWHNVNHRDEGEYQISRPEVDQVRVESEMRFAEMTNQGSNTEAVRNESGPNWKPPSPL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(51-317)


  Protein structure


Source ID Structure
AlphaFold DB A0A4Q2QTK9


T4CP

ID   4421 GenBank   WP_022650006
Name   trbC_FY206_RS25015_pLAP2_050004 insolico UniProt ID   _
Length   766 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 766 a.a.        Molecular weight: 87660.94 Da        Isoelectric Point: 6.8660

>WP_022650006.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MNYTPVNQALMKRNAWNSPVLELLRDHLLYAGIMAGSVVAGFIWPLAIPLLLLLAIVASISFGTHRWRMP
MRMPVHLNKVDPSEDRKVRRSLFRRFPSLFQYETTQESKGKGIFYLGYRRMNDIGRELWLTIDDLTRHVM
FFASTGGGKTETIYAWMLNSFCWGRGFTFVDGKAQNDTARTCYYLARRFGREDDVEYINFMNSGMSRSEM
IHNGDKSRPQSHQWNPFCYSTEAFVAETMQSMLPTNVQGGEWQSRAIAMNKALVFGTKFYCVRENKTMSL
QLLREFMPLEKLAELYCRAIDDQWPEEAVSPLYNYLVDVPGFDMATVRQPSAWTEEPRKQHSYLTGQFLE
TFNTFTETFGNIFAEDAGDIDIRDSIHSDRILLVLIPAMNTSQHTTSALGRMFVTQQSMILARDLGHRLE
GLDSEALEITKYKGPFPYMNYLDEVGAYYTERIAVQATQVRSLDFSIVMMSQDQERIESQTSATNVATLM
QNAGTKVAGKIVSDDKTAKTITNAAGQEARANMVSLQRRDGIIGTSWIDGDHINIQMENKINVQDLIKLQ
PGENYTVFQGDPVPGASFYIEPQDKTCNAPVIINRYITINPPNLKQLRKLVPRTAQRRLPAPENVSKIIG
VLTEKPSRRRKAAKAEPWKIIDTFQQRLANRQEAHNLMTEYDTDHRGREENLWEEALEVIRTTTMAERTI
RYITLNKPESEPTQVSAEEIQLAPDAILRTLTLPQPAYIPPSRYRNEPFISPKLPDSPHPDMEWPE

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(440-528)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 62119..96563

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FY206_RS25005 (FY206_25000) 59135..59338 - 204 WP_123839396 hypothetical protein -
FY206_RS25010 (FY206_25005) 59472..59741 - 270 WP_022650007 hypothetical protein -
FY206_RS25015 (FY206_25010) 59815..62115 - 2301 WP_022650006 F-type conjugative transfer protein TrbC -
FY206_RS25020 (FY206_25015) 62119..63375 - 1257 WP_126530067 hypothetical protein trbB
FY206_RS25025 (FY206_25020) 63356..64564 - 1209 WP_022650004 hypothetical protein trbA
FY206_RS25030 (FY206_25025) 64885..65517 - 633 WP_022650003 plasmid IncI1-type surface exclusion protein ExcA -
FY206_RS25035 (FY206_25030) 65579..67738 - 2160 WP_022650002 DotA/TraY family protein traY
FY206_RS25040 (FY206_25035) 67796..68338 - 543 WP_022650001 conjugal transfer protein TraX -
FY206_RS25045 (FY206_25040) 68335..69537 - 1203 WP_022650000 conjugal transfer protein TraW traW
FY206_RS25415 69550..70119 - 570 WP_022649999 hypothetical protein traV
FY206_RS25050 (FY206_25045) 70119..73172 - 3054 WP_022649998 hypothetical protein traU
FY206_RS25055 (FY206_25050) 73259..73984 - 726 WP_063864836 hypothetical protein traT
FY206_RS25060 (FY206_25055) 74168..74572 - 405 WP_022649996 DUF6750 family protein traR
FY206_RS25065 (FY206_25060) 74607..75131 - 525 WP_022649995 conjugal transfer protein TraQ traQ
FY206_RS25070 (FY206_25065) 75131..75904 - 774 WP_123839398 conjugal transfer protein TraP traP
FY206_RS25075 (FY206_25070) 75885..77192 - 1308 WP_022649993 conjugal transfer protein TraO traO
FY206_RS25080 (FY206_25075) 77192..78172 - 981 WP_022649992 DotH/IcmK family type IV secretion protein traN
FY206_RS25085 (FY206_25080) 78183..78800 - 618 WP_179297403 DotI/IcmL family type IV secretion protein traM
FY206_RS25090 (FY206_25085) 78890..79240 - 351 WP_022649990 hypothetical protein traL
FY206_RS25095 (FY206_25090) 79258..82800 - 3543 WP_032635082 LPD7 domain-containing protein -
FY206_RS25100 (FY206_25095) 82872..83159 - 288 WP_022649988 hypothetical protein traK
FY206_RS25105 (FY206_25100) 83156..84316 - 1161 WP_022649987 plasmid transfer ATPase TraJ virB11
FY206_RS25110 (FY206_25105) 84329..85135 - 807 WP_022649986 type IV secretory system conjugative DNA transfer family protein traI
FY206_RS25115 (FY206_25110) 85132..85605 - 474 WP_022649985 DotD/TraH family lipoprotein -
FY206_RS25120 (FY206_25115) 85676..86092 - 417 WP_123839394 hypothetical protein -
FY206_RS25125 (FY206_25120) 86201..87406 - 1206 WP_022649984 conjugal transfer protein TraF -
FY206_RS25130 (FY206_25125) 87512..88348 - 837 WP_022649983 hypothetical protein traE
FY206_RS25135 (FY206_25130) 88495..88971 - 477 WP_022649982 hypothetical protein -
FY206_RS25140 (FY206_25135) 88991..90151 - 1161 WP_022649981 site-specific integrase -
FY206_RS25145 (FY206_25140) 90729..92066 - 1338 WP_022649980 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
FY206_RS25150 (FY206_25145) 92063..92737 - 675 WP_022649979 prepilin peptidase -
FY206_RS25155 (FY206_25150) 92722..93204 - 483 WP_045261878 lytic transglycosylase domain-containing protein virB1
FY206_RS25160 (FY206_25155) 93289..93903 - 615 WP_022649977 type 4 pilus major pilin -
FY206_RS25165 (FY206_25160) 93917..95011 - 1095 WP_032623575 type II secretion system F family protein -
FY206_RS25170 (FY206_25165) 95004..96563 - 1560 WP_022649975 ATPase, T2SS/T4P/T4SS family virB11
FY206_RS25175 (FY206_25170) 96566..97081 - 516 WP_022649974 type IV pilus biogenesis protein PilP -
FY206_RS25180 (FY206_25175) 97065..98369 - 1305 WP_022649973 type 4b pilus protein PilO2 -
FY206_RS25185 (FY206_25180) 98371..100047 - 1677 WP_022649972 PilN family type IVB pilus formation outer membrane protein -
FY206_RS25190 (FY206_25185) 100062..100499 - 438 WP_022649971 type IV pilus biogenesis protein PilM -

Host bacterium

ID   7058 GenBank   NZ_CP043319
Plasmid name   pLAP2_050004 Incompatibility group   -
Plasmid size   106698 bp Coordinate of oriT [Strand]   55910..55996 [-]
Host baterium   Enterobacter chengduensis strain WCHECl-C4 = WCHECh050004

Cargo genes

Drug resistance gene   blaLAP-2, qnrS1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIC1, AcrIIA7