Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 106616
• Name: oriT_p2_CFSAN034202
• Organism: Clostridium botulinum strain CFSAN034202
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP031099 (5824..5849 [-], 26 nt)
• oriT length: 26 nt
• IRs (inverted repeats): 2..10, 17..25 (TAAAGTATA..TATACTTTA)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 26 nt

>oriT_p2_CFSAN034202
ATAAAGTATAATATGCTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_AGE31_RS19280_p2_CFSAN034202

• ID: 4388
• GenBank: WP_004441187
• Name: mobV_AGE31_RS19280_p2_CFSAN034202
• UniProt ID: _
• Length: 393 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 393 a.a.
• Molecular weight: 46243.16 Da
• Isoelectric Point: 7.1438

>WP_004441187.1 MobV family relaxase [Clostridium botulinum]
MSYLVCTMKKMKKDNLFGLQKHNQRESENHNNKDIDKNKSDLNYDLVNENKINLSEKVFDLIEKIRESEK
AIRKDAVLLDEFIISSDRDFFKNMNENEIKKFFETSKQFFAERYGEENIISANVHLDETTPHMHLDVCPL
RNGRLQSKNIFTRNELRNIQEELPKTLKNNGFEIERGVKGSTQKHIDIQTLKAREEKKLKELKEKNKEIE
LKIASRKEKLEEVKDLDFQIKSKKTVLNNLNQGNDCLDEDIKYKLDGFDVKMDYVEFRKFQTQIASIKKV
KKENEDLKNENSIVHKKCSDLRKSQEEVSSRFTNWIDNTYKHMSLDSREQFKRRGSESLANGSTGNYDED
STLDKSVMDSKAFNEDVYIKCANEKENALKEPKMSSNGFEIDW

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-192)

Protein structure

No available structure.

Host bacterium

• ID: 7053
• GenBank: NZ_CP031099
• Plasmid name: p2_CFSAN034202
• Incompatibility group: -
• Plasmid size: 5926 bp
• Coordinate of oriT [Strand]: 5824..5849 [-]
• Host baterium: Clostridium botulinum strain CFSAN034202

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -