Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106611
Name   oriT_CNSC001WB|unnamed1 in_silico
Organism   Latilactobacillus sakei strain CNSC001WB
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP113248 (2727..2746 [-], 20 nt)
oriT length   20 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 20 nt

>oriT_CNSC001WB|unnamed1
GTTGTGTGTAAGTGCGCATT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4387 GenBank   WP_278319970
Name   mobQ_OUY06_RS10305_CNSC001WB|unnamed1 insolico UniProt ID   _
Length   687 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 687 a.a.        Molecular weight: 80463.77 Da        Isoelectric Point: 9.1942

>WP_278319970.1 MobQ family relaxase [Latilactobacillus sakei]
MAIFHMSFQNISAGKGRSAIAGASYRSGEKLFDQKEGRSYFYARSVMPESFILTPKNAPEWASDREKLWN
EVERKDRRANSRYAKEFNVALPVELSEDEQKELLTKYVQENFVDEGMVADVAIHRDHPDNPHAHVMLTNR
PFNPDGTWGLKSKRENILDENGNKTYTGNSRFPRSRKVWLVDWDKKEKITEWRHNWAASVNQVLEQKNIP
DRISEKSFIEQGIDDTPTQHEGINSKRHERKEFNQQVKNYRKAKASYKNNQEKVINRGHLDSLSKHFSFN
EKRVVNELSHELKTYISLESLDDKRRMLFNWKNSTLIKHAVGEDVTKQLLTINQQESSLKKADELLNKVV
DRTTKKLYPELNFEQTTQAERRELIKETDSEQTVFKGSELNERLMNIRDDLLTQQLLTFTKRPYVGWKLL
MQQEKEVKIELKYTLMIHDDSLESLEHVDQGLLEKYSPTEQQKITRAVKDLRAIMAVKQVIQTQYHEVLK
RAFPKGDLDGLPLIKQEQAYTAVMYYDPVLKPCQAETIEQWQANPPQVFSPQEHQQGLAYLSGQLSLDQL
ENHHLQRVLKHDGTKQLFFGECKADPTIKNSQIEKIQKQLKGQQAKDDQYRKANIGHYQPLNYKPVSPDY
YLKTAFSNAIMTALYARDEDYQRQKQAQGLKETEWEMTKKQRQHQTRNRHEDGGMHL

  Protein domains


Predicted by InterproScan.

Nicking enzyme C-terminal middle helical domain (NES_C_h)

(276-384)

MobA/MobL family (MobA_MobL)

(17-241)


  Protein structure



No available structure.




T4CP

ID   4420 GenBank   WP_252814983
Name   t4cp2_OUY06_RS10365_CNSC001WB|unnamed1 insolico UniProt ID   _
Length   516 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 516 a.a.        Molecular weight: 57996.16 Da        Isoelectric Point: 4.8756

>WP_252814983.1 VirD4-like conjugal transfer protein, CD1115 family [Latilactobacillus sakei]
MNGTILGIVDKQIVYQNNSTKPNRNIFVVGGPGSYKTQSVVITNLFNETENSIVVTDPKGELYEKTAGIK
LAQGYQVHVVNFANMAHSDRYNPFDYIQRDIQAETVATKIVQSENAEGKKDVWFSTQRQLLKALILFVMN
HRAPDQRNLAGVTQVLQENDVEAEEKGADSPLDTLFLDLTMTDPARRAYELGFKKAKGEMKASIIESLLA
TISKFVDKEVADFTSFSDFDLKEIGNDKVVLYVIIPVMDNTYESFINLFFSQLFDELYKLAADHHAKLPH
QVDFILDEFVNLGKFPKYEEFLATCRGYGIGVTTICQTLTQLQALYGKDKAESILGNHAVKICLNAANDV
TAKYFSDLLGKSTVKVETGSESTSHSKEESHSKSDSYSYTSRSLMTPDEIMRMPEDQSLLIFSNARPVKA
TKAFQFKLFPGADHLVNLSQNDYQGQPEASQETNFKNKVEKWKAKLKETAQKEVANKISQKEEEQQQDNL
DSDLERVSNKDSQTEAPEEDDNNLLG

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(24-443)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 6322..16177

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OUY06_RS10295 2067..2345 - 279 WP_057904517 hypothetical protein -
OUY06_RS10300 2368..2595 - 228 WP_076639168 hypothetical protein -
OUY06_RS10305 2849..4912 + 2064 WP_278319970 MobQ family relaxase -
OUY06_RS10310 4997..5308 + 312 WP_039098894 hypothetical protein -
OUY06_RS10315 5350..5964 + 615 WP_252814977 hypothetical protein -
OUY06_RS10320 5966..6301 + 336 WP_252814978 CagC family type IV secretion system protein -
OUY06_RS10325 6322..6684 + 363 WP_014216295 hypothetical protein trsC
OUY06_RS10330 6653..7312 + 660 WP_112217880 TrsD/TraD family conjugative transfer protein trsD
OUY06_RS10335 7324..9339 + 2016 WP_252814979 DUF87 domain-containing protein virb4
OUY06_RS10340 9332..10750 + 1419 WP_252814980 type VII secretion protein EssB/YukC trsF
OUY06_RS10345 10751..11905 + 1155 WP_278319971 phage tail tip lysozyme orf14
OUY06_RS10350 11920..12537 + 618 WP_252814982 hypothetical protein -
OUY06_RS10355 12524..12892 + 369 WP_154255305 thioredoxin family protein -
OUY06_RS10360 12893..13363 + 471 WP_076801968 conjugal transfer protein trsJ
OUY06_RS10365 13365..14915 + 1551 WP_252814983 VirD4-like conjugal transfer protein, CD1115 family -
OUY06_RS10370 14930..15319 + 390 WP_076981471 hypothetical protein -
OUY06_RS10375 15338..16177 + 840 WP_252814984 conjugal transfer protein TrbL family protein trsL
OUY06_RS10380 16193..16603 + 411 WP_278319972 hypothetical protein -
OUY06_RS10385 16610..18745 + 2136 WP_278319973 DNA topoisomerase III -
OUY06_RS10390 18867..19082 + 216 WP_252814987 hypothetical protein -
OUY06_RS10395 19086..20210 + 1125 WP_252814988 ArdC-like ssDNA-binding domain-containing protein -

Host bacterium

ID   7048 GenBank   NZ_CP113248
Plasmid name   CNSC001WB|unnamed1 Incompatibility group   -
Plasmid size   48679 bp Coordinate of oriT [Strand]   2727..2746 [-]
Host baterium   Latilactobacillus sakei strain CNSC001WB

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21