Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106508
Name   oriT_pIHI2-2152 in_silico
Organism   Enterobacter hormaechei strain Y2152
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP049193 (149412..149666 [+], 255 nt)
oriT length   255 nt
IRs (inverted repeats)      153..158, 160..165  (AAAAGT..ACTTTT)
 122..130, 135..143  (TTAAGGCTT..AAGCCTTAA)
 49..55, 60..66  (GAATTTT..AAAATTC)
Location of nic site      78..79
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 255 nt

>oriT_pIHI2-2152
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4335 GenBank   WP_012477394
Name   mobH_G5577_RS23350_pIHI2-2152 insolico UniProt ID   A0A2Z2E7B6
Length   1050 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1050 a.a.        Molecular weight: 118067.47 Da        Isoelectric Point: 4.6347

>WP_012477394.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDVITKITDT
VETAVLKVNDLGRSSASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDTTMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFSQLSVSRPEVG
SCATSSSVHNEKLLSEPSELPELNREQNADPQGTNERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFASTESDQNGEFSLISEGDVTELEFVEIALVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(108-304)


  Protein structure


Source ID Structure
AlphaFold DB A0A2Z2E7B6


T4CP

ID   4357 GenBank   WP_001284073
Name   traD_G5577_RS23345_pIHI2-2152 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 78123.91 Da        Isoelectric Point: 8.0177

>WP_001284073.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(406-609)

AAA-like domain (AAA_10)

(199-249)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 294181..303388

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5577_RS24180 (G5577_24185) 290526..290843 + 318 Protein_337 RepB family plasmid replication initiator protein -
G5577_RS24190 (G5577_24195) 292015..292626 + 612 WP_224743090 RepB family plasmid replication initiator protein -
G5577_RS24680 293776..293916 + 141 WP_242700050 hypothetical protein -
G5577_RS24685 293957..294130 + 174 WP_242700051 hypothetical protein -
G5577_RS24200 (G5577_24205) 294148..294498 + 351 WP_001447770 type IV conjugative transfer system protein TraL -
G5577_RS24690 294510..294653 + 144 WP_242700052 hypothetical protein -
G5577_RS24205 (G5577_24210) 294744..295298 + 555 WP_341871323 TraE/TraK family type IV conjugative transfer system protein -
G5577_RS24210 (G5577_24215) 295298..296569 + 1272 WP_000592092 type-F conjugative transfer system secretin TraK traK
G5577_RS24215 (G5577_24220) 296571..297032 + 462 WP_000521242 plasmid transfer protein HtdO -
G5577_RS24220 (G5577_24225) 297022..298377 + 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
G5577_RS24225 (G5577_24230) 298385..298867 + 483 WP_000377632 hypothetical protein -
G5577_RS24230 (G5577_24235) 298986..299738 + 753 WP_182911723 protein-disulfide isomerase HtdT -
G5577_RS24235 (G5577_24240) 299748..300698 + 951 WP_001022588 IncHI-type conjugal transfer lipoprotein TrhV traV
G5577_RS24240 (G5577_24245) 300707..303388 + 2682 WP_000387412 TraC family protein virb4

Host bacterium

ID   6945 GenBank   NZ_CP049193
Plasmid name   pIHI2-2152 Incompatibility group   IncHI2
Plasmid size   303394 bp Coordinate of oriT [Strand]   149412..149666 [+]
Host baterium   Enterobacter hormaechei strain Y2152

Cargo genes

Drug resistance gene   catA2, tet(D), aph(3')-Ia, mcr-9, aph(6)-Id, aph(3'')-Ib, dfrA19, sul1, blaDHA-1, qnrB4, qacE, ere(A), aac(6')-IIc
Virulence gene   -
Metal resistance gene   terW, terZ, terA, terB, terC, terD, terE, merR1, merT, merP, merA, merB, merR2, merD, merE, arsH, arsR, arsB, arsC, rcnR/yohL, ncrC
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -