Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 106506 |
| Name | oriT_pNY13320-2  |
| Organism | Enterococcus faecium strain NY13320 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP094232 (27926..28117 [-], 192 nt) |
| oriT length | 192 nt |
| IRs (inverted repeats) | 106..112, 122..128 (ATTTTTT..AAAAAAT) 107..113, 120..126 (TTTTTTG..CAAAAAA) 93..98, 105..110 (AAAATA..TATTTT) 65..71, 75..81 (AGTTGGC..GCCAACT) 41..49, 52..60 (CACCTTCCT..AGGAAGGTG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 192 nt
>oriT_pNY13320-2
CCAAGGAATTAATACAAAGAGCATAAGGGAAAACTGATAGCACCTTCCTAAAGGAAGGTGGCTAAGTTGGCTGTGCCAACTGGTTTTCTTTCAAAATAGCTTCATATTTTTTGCTATCACAAAAAAATCCATTTTCGACCTATTTTCGGTCATAATATAGTACCTACTTTTGGTCATAGTTTCGTCCGTAGT
CCAAGGAATTAATACAAAGAGCATAAGGGAAAACTGATAGCACCTTCCTAAAGGAAGGTGGCTAAGTTGGCTGTGCCAACTGGTTTTCTTTCAAAATAGCTTCATATTTTTTGCTATCACAAAAAAATCCATTTTCGACCTATTTTCGGTCATAATATAGTACCTACTTTTGGTCATAGTTTCGTCCGTAGT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 4334 | GenBank | WP_002326870 |
| Name | mobP2_MPN36_RS15000_pNY13320-2 |
UniProt ID | B5U8V6 |
| Length | 506 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 506 a.a. Molecular weight: 60597.01 Da Isoelectric Point: 9.3485
>WP_002326870.1 MULTISPECIES: MobP2 family relaxase [Enterococcus]
MTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDYISRQEAIRQDKLDHDYSEDELNELARIEKAL
EKIEQETDQPVIKDLREMDKYIDYMTRKKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNG
SVMFQDVISFDNDFLVREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHV
TAMERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLELKQSVKDSLI
QLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDNPKRKEYDRMTKEIDEL
YQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLLKQSEEKNLKSNELKNLLDGKKLNVKFDREKPEF
KTKYAKQSYDDFQKRLEERQLAREKNEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMI
EQEKMRQAREFEIGMN
MTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDYISRQEAIRQDKLDHDYSEDELNELARIEKAL
EKIEQETDQPVIKDLREMDKYIDYMTRKKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNG
SVMFQDVISFDNDFLVREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHV
TAMERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLELKQSVKDSLI
QLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDNPKRKEYDRMTKEIDEL
YQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLLKQSEEKNLKSNELKNLLDGKKLNVKFDREKPEF
KTKYAKQSYDDFQKRLEERQLAREKNEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMI
EQEKMRQAREFEIGMN
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | B5U8V6 |
T4CP
| ID | 4356 | GenBank | WP_123061283 |
| Name | t4cp2_MPN36_RS14930_pNY13320-2 |
UniProt ID | _ |
| Length | 952 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 952 a.a. Molecular weight: 108310.19 Da Isoelectric Point: 7.4752
>WP_123061283.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecium]
MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIEGEDLPKSLQDKLNVPLVFIGVFLGSI
VFLAITYLLNFLKALLVAIGQSESGLLGVEFDPKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNH
ESEKNLVYGQKGDSRLSTIKEIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGK
GETFVVPLLDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVIDSWA
NKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDCIEKITMYNVAQMLNEL
GSLFYTDPKTRKEKNALDEYFNTLPQGNQAKIQYGSTSFAGDKAKGSILSTASQGIEMFTSDLFGKLTSK
MSIDLKEIGFPKSIQFKVNTNLVGKRISISFLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLD
IRYEEEKSRAWYRIEFPKKQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNH
VLASIFISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVIQSYSQL
ETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVE
RASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEIDIACEHINLSL
QDNQIPYARFIKDLKTRLEYSIINNIPISEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLS
EQEMKINIYLKESQKEAIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQ
IMILEQQLANAYELAVFKTKNSILNNKIIKLKNAMTKLAKAI
MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIEGEDLPKSLQDKLNVPLVFIGVFLGSI
VFLAITYLLNFLKALLVAIGQSESGLLGVEFDPKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNH
ESEKNLVYGQKGDSRLSTIKEIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGK
GETFVVPLLDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVIDSWA
NKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDCIEKITMYNVAQMLNEL
GSLFYTDPKTRKEKNALDEYFNTLPQGNQAKIQYGSTSFAGDKAKGSILSTASQGIEMFTSDLFGKLTSK
MSIDLKEIGFPKSIQFKVNTNLVGKRISISFLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLD
IRYEEEKSRAWYRIEFPKKQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNH
VLASIFISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVIQSYSQL
ETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVE
RASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEIDIACEHINLSL
QDNQIPYARFIKDLKTRLEYSIINNIPISEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLS
EQEMKINIYLKESQKEAIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQ
IMILEQQLANAYELAVFKTKNSILNNKIIKLKNAMTKLAKAI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 8378..26014
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MPN36_RS14865 (MPN36_14865) | 6681..7043 | + | 363 | WP_002311845 | hypothetical protein | - |
| MPN36_RS14870 (MPN36_14870) | 7057..7209 | + | 153 | WP_002311846 | hypothetical protein | - |
| MPN36_RS14875 (MPN36_14875) | 7277..7699 | + | 423 | WP_002311847 | hypothetical protein | - |
| MPN36_RS14880 (MPN36_14880) | 7711..8370 | + | 660 | WP_242923955 | hypothetical protein | - |
| MPN36_RS14885 (MPN36_14885) | 8378..9124 | + | 747 | WP_242923956 | CpaF/VirB11 family protein | virB11 |
| MPN36_RS14890 (MPN36_14890) | 9141..9956 | + | 816 | WP_002313315 | hypothetical protein | - |
| MPN36_RS14895 (MPN36_14895) | 9956..10753 | + | 798 | WP_002311850 | hypothetical protein | - |
| MPN36_RS14900 (MPN36_14900) | 10771..11133 | + | 363 | WP_002311851 | DUF4320 family protein | - |
| MPN36_RS14905 (MPN36_14905) | 11147..11293 | + | 147 | WP_002326866 | hypothetical protein | - |
| MPN36_RS14910 (MPN36_14910) | 11305..11526 | + | 222 | WP_002326867 | hypothetical protein | - |
| MPN36_RS14915 (MPN36_14915) | 11545..11820 | + | 276 | WP_002311852 | TrbC/VirB2 family protein | virB2 |
| MPN36_RS14920 (MPN36_14920) | 11929..13755 | + | 1827 | WP_002311853 | hypothetical protein | - |
| MPN36_RS14925 (MPN36_14925) | 13768..14484 | + | 717 | WP_002311854 | hypothetical protein | - |
| MPN36_RS14930 (MPN36_14930) | 14497..17355 | + | 2859 | WP_123061283 | VirD4-like conjugal transfer protein, CD1115 family | - |
| MPN36_RS14935 (MPN36_14935) | 17373..20057 | + | 2685 | WP_227889218 | pLS20_p028 family conjugation system transmembrane protein | - |
| MPN36_RS14940 (MPN36_14940) | 20105..20416 | + | 312 | WP_002317413 | DUF5592 family protein | - |
| MPN36_RS14945 (MPN36_14945) | 20413..21030 | + | 618 | WP_002311858 | hypothetical protein | virb4 |
| MPN36_RS14950 (MPN36_14950) | 21047..21514 | + | 468 | WP_002311859 | hypothetical protein | - |
| MPN36_RS14955 (MPN36_14955) | 21530..23155 | + | 1626 | WP_010729563 | hypothetical protein | virb4 |
| MPN36_RS14960 (MPN36_14960) | 24016..24348 | + | 333 | WP_242923957 | HNH endonuclease signature motif containing protein | - |
| MPN36_RS14965 (MPN36_14965) | 24536..24856 | + | 321 | WP_010729568 | hypothetical protein | virb4 |
| MPN36_RS14970 (MPN36_14970) | 24878..26014 | + | 1137 | WP_002311861 | bifunctional lytic transglycosylase/C40 family peptidase | orf14 |
| MPN36_RS14975 (MPN36_14975) | 26029..26679 | + | 651 | WP_002311862 | cell division protein FtsL | - |
| MPN36_RS14980 (MPN36_14980) | 26693..27589 | + | 897 | WP_123061271 | hypothetical protein | - |
| MPN36_RS14985 (MPN36_14985) | 27625..27855 | + | 231 | WP_002311864 | hypothetical protein | - |
| MPN36_RS14990 (MPN36_14990) | 28206..28502 | + | 297 | WP_002311865 | hypothetical protein | - |
| MPN36_RS14995 (MPN36_14995) | 28499..28966 | + | 468 | WP_002311867 | hypothetical protein | - |
| MPN36_RS15000 (MPN36_15000) | 29048..30568 | + | 1521 | WP_002326870 | MobP2 family relaxase | - |
| MPN36_RS15005 (MPN36_15005) | 30590..30919 | + | 330 | WP_002311869 | hypothetical protein | - |
Host bacterium
| ID | 6943 | GenBank | NZ_CP094232 |
| Plasmid name | pNY13320-2 | Incompatibility group | - |
| Plasmid size | 58545 bp | Coordinate of oriT [Strand] | 27926..28117 [-] |
| Host baterium | Enterococcus faecium strain NY13320 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA1 |