Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106503
Name   oriT_SAL-007|unnamed2 in_silico
Organism   Salmonella sp. SAL-007
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP071688 (24057..24663 [+], 607 nt)
oriT length   607 nt
IRs (inverted repeats)      590..595, 600..605  (ATGCCC..GGGCAT)
 517..523, 529..535  (CCTCCCG..CGGGAGG)
 453..458, 462..467  (GTTCGC..GCGAAC)
 196..202, 204..210  (TCAATTC..GAATTGA)
 175..180, 187..192  (AAATCA..TGATTT)
 103..108, 120..125  (GCTAAA..TTTAGC)
 79..84, 93..98  (GCTTTA..TAAAGC)
 29..37, 46..54  (TTTTGATAA..TTATCAAAA)
Location of nic site      313..314
Conserved sequence flanking the
  nic site    		  TCCTGCATCG
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 607 nt

>oriT_SAL-007|unnamed2
CTTTGTTTACCTGTTAAGTACATCGTTGTTTTGATAAAATCATTATTATCAAAAAACGTATTTATACCCTTACTGCCAGCTTTACTTTTTAATAAAGCATTTGCTAAAAGTTTCATGCCTTTAGCTACTGTTAAAGCTGGTTCATCTGGATAAAGAGTTTTAAGGCAATCCAGCAAATCATCATGCTGATTTTCATCAATTCTGAATTGAACTTTTTTCATAAAATTCTCCGACAAGAACCGACTGTAGGTCACCGGGCAAACGTTGCGGAATGGCGTTAGAGACGTCATTTTGCGGCGTTTGCCCTATCCTGCATCGCAGTGGATCTGGCACCGCTCATAAATTGTGCTTGAGCATCAACAAAAATGCAAAAAGGCCGATGTTGTCATGGCTGAACATACGTGCGTCTGCGACCGGAAACTCGATCAATGCGGTAAGGACAGTGCCGTCAGGTTCGCTCCGCGAACGGTCTGGCAGGAAGCGCAAGCGCAGTCCCGCGCAATATATCAGAAAGCACCTCCCGAAATACGGGAGGGCTTCGGCTATTCAGCTAAGGTTGCTCTGTTCAGGTTGCTGAGTGTAGCCTTCCATGCCCTGACGGGCATCA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4332 GenBank   WP_000539539
Name   mobP1_J1G46_RS23180_SAL-007|unnamed2 insolico UniProt ID   A0A748H4F7
Length   388 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 388 a.a.        Molecular weight: 44419.77 Da        Isoelectric Point: 10.5368

>WP_000539539.1 MULTISPECIES: MobP1 family relaxase [Enterobacteriaceae]
MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAVTRQGIRNSI
DYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENKKITQNIVFSPPVSAKAKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GGLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFWGL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(70-227)


  Protein structure


Source ID Structure
AlphaFold DB A0A748H4F7


Host bacterium

ID   6940 GenBank   NZ_CP071688
Plasmid name   SAL-007|unnamed2 Incompatibility group   IncX1
Plasmid size   29336 bp Coordinate of oriT [Strand]   24057..24663 [+]
Host baterium   Salmonella sp. SAL-007

Cargo genes

Drug resistance gene   blaTEM-1B, aph(6)-Id, aph(3'')-Ib, tet(A), sul2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -