Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 106501 |
| Name | oriT_ES-1|unnamed2  |
| Organism | Enterococcus faecalis strain ES-1 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP049777 (16752..16781 [-], 30 nt) |
| oriT length | 30 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 30 nt
>oriT_ES-1|unnamed2
AACGTAAGTTAGTGGGTATAAGTGCGCCCT
AACGTAAGTTAGTGGGTATAAGTGCGCCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 4330 | GenBank | WP_242693023 |
| Name | mobQ_G7X26_RS14300_ES-1|unnamed2 |
UniProt ID | _ |
| Length | 664 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 664 a.a. Molecular weight: 77704.92 Da Isoelectric Point: 9.3434
>WP_242693023.1 MULTISPECIES: MobQ family relaxase [Enterococcus]
MIQLAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTIKPEAFILKPDYVPDEFLDR
QTLWNKMELAEKSPNAQLCREVNVALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHI
MLTMREVDSEGNILNKRKRIPKLDENGNQIFNEKGQRVTVSIKTNDWDRKSLVSEIRKDWADKVNQYLKD
RNIDQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDILAELDKLESQEKVLKQ
DQNFTLKFEKTFSPLEKGELKNLSKELKLFINDENIDKRLGELKRWENSLIFNNKMEIQKQRLMLSKISS
ERDMLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVIEKEE
SKKIFKEKPFQTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVS
IDSVIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMK
YIDELSPLAKHNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRL
LSGNQLQKKRNLDKLIKQTKAKKNQSLQRNIPLR
MIQLAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTIKPEAFILKPDYVPDEFLDR
QTLWNKMELAEKSPNAQLCREVNVALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHI
MLTMREVDSEGNILNKRKRIPKLDENGNQIFNEKGQRVTVSIKTNDWDRKSLVSEIRKDWADKVNQYLKD
RNIDQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDILAELDKLESQEKVLKQ
DQNFTLKFEKTFSPLEKGELKNLSKELKLFINDENIDKRLGELKRWENSLIFNNKMEIQKQRLMLSKISS
ERDMLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVIEKEE
SKKIFKEKPFQTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVS
IDSVIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMK
YIDELSPLAKHNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRL
LSGNQLQKKRNLDKLIKQTKAKKNQSLQRNIPLR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 4353 | GenBank | WP_165548720 |
| Name | t4cp2_G7X26_RS14345_ES-1|unnamed2 |
UniProt ID | _ |
| Length | 551 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 551 a.a. Molecular weight: 63162.23 Da Isoelectric Point: 4.7351
>WP_165548720.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Enterococcus]
MSSLLAESDGLILGKFQSGNVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLNLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVAEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQYYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVTEEEEEKELLMSDILSSFDEGIEENTTNNDNSELPF
MSSLLAESDGLILGKFQSGNVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLNLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVAEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQYYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVTEEEEEKELLMSDILSSFDEGIEENTTNNDNSELPF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 19247..39107
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| G7X26_RS14280 (G7X26_14280) | 15177..15674 | + | 498 | WP_071621495 | DnaJ domain-containing protein | - |
| G7X26_RS14285 (G7X26_14285) | 15708..16013 | + | 306 | WP_033626036 | hypothetical protein | - |
| G7X26_RS14290 (G7X26_14290) | 16036..16293 | - | 258 | WP_002325623 | hypothetical protein | - |
| G7X26_RS14295 (G7X26_14295) | 16296..16595 | - | 300 | WP_002325624 | hypothetical protein | - |
| G7X26_RS14300 (G7X26_14300) | 16887..18872 | + | 1986 | WP_165548714 | MobQ family relaxase | - |
| G7X26_RS14305 (G7X26_14305) | 18896..19228 | + | 333 | WP_000713503 | CagC family type IV secretion system protein | - |
| G7X26_RS14310 (G7X26_14310) | 19247..19630 | + | 384 | WP_165548716 | glycosyltransferase | trsC |
| G7X26_RS14315 (G7X26_14315) | 19647..20276 | + | 630 | WP_002301630 | hypothetical protein | trsD |
| G7X26_RS14320 (G7X26_14320) | 20287..22248 | + | 1962 | WP_165548718 | TrsE protein | virb4 |
| G7X26_RS14325 (G7X26_14325) | 22262..23614 | + | 1353 | WP_104659242 | conjugal transfer protein TraF | trsF |
| G7X26_RS14330 (G7X26_14330) | 23636..24745 | + | 1110 | WP_021109240 | lysozyme family protein | orf14 |
| G7X26_RS14335 (G7X26_14335) | 24758..25309 | + | 552 | WP_021109241 | hypothetical protein | - |
| G7X26_RS14340 (G7X26_14340) | 25314..25745 | + | 432 | WP_002301637 | hypothetical protein | - |
| G7X26_RS14345 (G7X26_14345) | 25738..27393 | + | 1656 | WP_165548720 | VirD4-like conjugal transfer protein, CD1115 family | - |
| G7X26_RS14350 (G7X26_14350) | 27411..28334 | + | 924 | WP_165548721 | hypothetical protein | - |
| G7X26_RS14355 (G7X26_14355) | 28334..29266 | + | 933 | WP_002377193 | conjugal transfer protein TrbL family protein | trsL |
| G7X26_RS14360 (G7X26_14360) | 29283..30251 | + | 969 | WP_165548723 | hypothetical protein | - |
| G7X26_RS14365 (G7X26_14365) | 30280..30633 | + | 354 | WP_002339801 | hypothetical protein | - |
| G7X26_RS14370 (G7X26_14370) | 30693..31070 | - | 378 | WP_094899408 | hypothetical protein | - |
| G7X26_RS14375 (G7X26_14375) | 31317..31562 | + | 246 | WP_047927842 | hypothetical protein | - |
| G7X26_RS14380 (G7X26_14380) | 32056..33603 | + | 1548 | WP_094899406 | type I restriction-modification system subunit M | - |
| G7X26_RS14385 (G7X26_14385) | 33600..34835 | + | 1236 | WP_242693022 | restriction endonuclease subunit S | - |
| G7X26_RS14390 (G7X26_14390) | 34838..37936 | + | 3099 | WP_165548725 | type I restriction endonuclease subunit R | - |
| G7X26_RS14520 | 38349..39107 | + | 759 | WP_207623151 | LPXTG cell wall anchor domain-containing protein | prgC |
| G7X26_RS14400 (G7X26_14400) | 39222..40116 | + | 895 | Protein_40 | IS256 family transposase | - |
| G7X26_RS14405 (G7X26_14405) | 40596..41168 | + | 573 | WP_002292150 | DNA starvation/stationary phase protection protein | - |
| G7X26_RS14410 (G7X26_14410) | 41313..42017 | + | 705 | WP_002333366 | Crp/Fnr family transcriptional regulator | - |
| G7X26_RS14415 (G7X26_14415) | 42041..42334 | + | 294 | WP_156272407 | prolipoprotein diacylglyceryl transferase family protein | - |
| G7X26_RS14420 (G7X26_14420) | 42481..42714 | + | 234 | WP_002292161 | heavy-metal-associated domain-containing protein | - |
Host bacterium
| ID | 6938 | GenBank | NZ_CP049777 |
| Plasmid name | ES-1|unnamed2 | Incompatibility group | - |
| Plasmid size | 53121 bp | Coordinate of oriT [Strand] | 16752..16781 [-] |
| Host baterium | Enterococcus faecalis strain ES-1 |
Cargo genes
| Drug resistance gene | fosB3, erm(B), aac(6')-aph(2'') |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |