Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 106477
• Name: oriT_plas1.1.1
• Organism: Proteus mirabilis strain SCBX1.1
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP047113 (2416..2456 [+], 41 nt)
• oriT length: 41 nt
• IRs (inverted repeats): 14..22, 29..37 (TAAAGTATA..TATACTTTA) 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 41 nt

>oriT_plas1.1.1
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_GO498_RS19615_plas1.1.1

• ID: 4318
• GenBank: WP_012779614
• Name: mobV_GO498_RS19615_plas1.1.1
• UniProt ID: C7C2U1
• Length: 408 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 408 a.a.
• Molecular weight: 48229.55 Da
• Isoelectric Point: 7.0097

>WP_012779614.1 MULTISPECIES: MobV family relaxase [Bacteria]
MSMIVARMQKMKAENLVGIGNHNQRKTKNHSNPDIDTSLSKLNYDLVDRTQNYKTDIENFINENKSTTRA
VRKDAVLVNEWIISSDKDFFDNLTESEIENFFERSKDYFAEKFGEKNIRYATVHLDESTPHMHMGIVPFD
KDNKLSAKRVFNRQALRDVQEELPKYLQDFQFEIARGQKGSERKNLTVPEFKKLKEEELEIKKELQIKKD
ELIAYTKENQIDKKLDITPIKEMEDIEIETDEKTLFGKNKTEIVRQWTGNIILSENDYLKLNKEIKKGKK
TEGRLAAILETDVYQENKELKNELKDQIDKNDKDIDDYNDLVKRYNNLYEENTSLKSQIGDLKEEIKLIY
QSTKRFLKDRISDFKAFKEVFKELADNISNISREKGLDSSFKKEFDRENKKKQTRGIR

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

• AlphaFold DB: C7C2U1

Host bacterium

• ID: 6914
• GenBank: NZ_CP047113
• Plasmid name: plas1.1.1
• Incompatibility group: ColRNAI
• Plasmid size: 12795 bp
• Coordinate of oriT [Strand]: 2416..2456 [+]
• Host baterium: Proteus mirabilis strain SCBX1.1

Cargo genes

• Drug resistance gene: cfr
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -