Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 106476
• Name: oriT_pWX8
• Organism: Lacticaseibacillus paracasei strain WX322
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP044244 (2824..2860 [-], 37 nt)
• oriT length: 37 nt
• IRs (inverted repeats): 14..21, 28..35 (AAAGTATA..TATACTTT) 1..6, 13..18 (ACTTTG..CAAAGT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 37 nt

>oriT_pWX8
ACTTTGTGATAGCAAAGTATACTGGGTTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_F0M22_RS16165_pWX8

• ID: 4317
• GenBank: WP_242756251
• Name: mobV_F0M22_RS16165_pWX8
• UniProt ID: _
• Length: 436 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 436 a.a.
• Molecular weight: 50760.97 Da
• Isoelectric Point: 7.2339

>WP_242756251.1 MobV family relaxase [Lacticaseibacillus paracasei]
MSQLAAHMQKFKIGNLGGLQRHDERTLQHHSNPDIDVSKSLDNFSVLPPERLDRSRSLHKQVQEIIADER
VSNRAVRKDAVVLTEWVISSDSAFFEGLSSDETERFFTDAYQWFANHFGADHIPYATVHMDETTPHMHMG
VIPLTNGRLSAKTIFNREALRFIQADLPQKLNEKGWNIERGERGSKRKHLSIERYKEVAESLKQGEPLFD
VSKLEHERVPIVNMFNFVKGKEATQNYILPPDDYQKLTERVRGFSDLGHAVKDVNDLKAALNARESLIRQ
REKELDKREDAISKNENLIETYDFWYDQAYEQSNKVTQLQGQIKTLKAENKQWQQHYYKLVDAVHDMAKT
IGTLIADYPAWEQKLNDFGQRVIRGTRAFAKNTLNKVQAYGAAEDIDTTVEISKNIEPDMYPEREQERRQ
RLQQQRKRQQQQGLER

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (4-197)

Protein structure

No available structure.

Host bacterium

• ID: 6913
• GenBank: NZ_CP044244
• Plasmid name: pWX8
• Incompatibility group: -
• Plasmid size: 3077 bp
• Coordinate of oriT [Strand]: 2824..2860 [-]
• Host baterium: Lacticaseibacillus paracasei strain WX322

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -