Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106452
Name   oriT_pESBL-PH-57 in_silico
Organism   Klebsiella pneumoniae strain UHD-57_PH
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP121144 (69331..69380 [+], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_pESBL-PH-57
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4301 GenBank   WP_015065542
Name   traI_P8T33_RS25990_pESBL-PH-57 insolico UniProt ID   K7R3U7
Length   1752 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1752 a.a.        Molecular weight: 190318.03 Da        Isoelectric Point: 6.2890

>WP_015065542.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacterales]
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMSREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALAVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAAGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDTAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGGGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD

  Protein domains


Predicted by InterproScan.

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(576-628)

DNA helicase TraI (TraI_C)

(1432-1554)

TrwC relaxase (TrwC)

(11-283)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(634-713)

AAA domain (AAA_30)

(970-1156)


  Protein structure



No available structure.




T4CP

ID   4330 GenBank   WP_015065541
Name   traD_P8T33_RS25995_pESBL-PH-57 insolico UniProt ID   _
Length   770 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 770 a.a.        Molecular weight: 86025.00 Da        Isoelectric Point: 5.0577

>WP_015065541.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 39924..69938

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P8T33_RS25995 (P8T33_25990) 39924..42236 - 2313 WP_015065541 type IV conjugative transfer system coupling protein TraD virb4
P8T33_RS26000 (P8T33_25995) 42365..43054 - 690 WP_015065540 hypothetical protein -
P8T33_RS26005 (P8T33_26000) 43246..43977 - 732 WP_032329926 conjugal transfer complement resistance protein TraT -
P8T33_RS26010 (P8T33_26005) 44286..44810 - 525 WP_015065538 hypothetical protein -
P8T33_RS26015 (P8T33_26010) 44821..47664 - 2844 WP_023157999 conjugal transfer mating-pair stabilization protein TraG traG
P8T33_RS26020 (P8T33_26015) 47664..49043 - 1380 WP_023158000 conjugal transfer pilus assembly protein TraH traH
P8T33_RS26025 (P8T33_26020) 49021..49464 - 444 WP_023158001 F-type conjugal transfer protein TrbF -
P8T33_RS26030 (P8T33_26025) 49510..50067 - 558 WP_023158002 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
P8T33_RS26035 (P8T33_26030) 50039..50278 - 240 WP_023158003 type-F conjugative transfer system pilin chaperone TraQ -
P8T33_RS26040 (P8T33_26035) 50289..51041 - 753 WP_015065535 type-F conjugative transfer system pilin assembly protein TraF traF
P8T33_RS26045 (P8T33_26040) 51062..51388 - 327 WP_015065534 hypothetical protein -
P8T33_RS26050 (P8T33_26045) 51401..51649 - 249 WP_004152675 hypothetical protein -
P8T33_RS26055 (P8T33_26050) 51627..51881 - 255 WP_015065558 conjugal transfer protein TrbE -
P8T33_RS26060 (P8T33_26055) 51913..53868 - 1956 WP_015065533 type-F conjugative transfer system mating-pair stabilization protein TraN traN
P8T33_RS26065 (P8T33_26060) 53927..54574 - 648 WP_015632510 type-F conjugative transfer system pilin assembly protein TrbC trbC
P8T33_RS26070 (P8T33_26065) 54653..54829 - 177 WP_162866177 hypothetical protein -
P8T33_RS26075 (P8T33_26070) 54850..55839 - 990 WP_015632509 conjugal transfer pilus assembly protein TraU traU
P8T33_RS26080 (P8T33_26075) 55836..56237 - 402 WP_023158005 hypothetical protein -
P8T33_RS26085 (P8T33_26080) 56272..56907 - 636 WP_023158006 type-F conjugative transfer system protein TraW traW
P8T33_RS26090 (P8T33_26085) 56907..57296 - 390 WP_004167468 type-F conjugative transfer system protein TrbI -
P8T33_RS26095 (P8T33_26090) 57296..59935 - 2640 WP_023158007 type IV secretion system protein TraC virb4
P8T33_RS26100 (P8T33_26095) 60007..60405 - 399 WP_023158008 hypothetical protein -
P8T33_RS26105 (P8T33_26100) 60413..60802 - 390 WP_023158009 hypothetical protein -
P8T33_RS26110 (P8T33_26105) 60845..61249 - 405 WP_016831042 hypothetical protein -
P8T33_RS26115 (P8T33_26110) 61316..61627 - 312 WP_014386200 hypothetical protein -
P8T33_RS26120 (P8T33_26115) 61628..61846 - 219 WP_014386199 hypothetical protein -
P8T33_RS26125 (P8T33_26120) 61870..62159 - 290 Protein_69 hypothetical protein -
P8T33_RS26130 (P8T33_26125) 62164..62574 - 411 WP_016831039 hypothetical protein -
P8T33_RS26135 (P8T33_26130) 62706..63290 - 585 WP_014386196 type IV conjugative transfer system lipoprotein TraV traV
P8T33_RS26140 (P8T33_26135) 63313..63909 - 597 Protein_72 conjugal transfer pilus-stabilizing protein TraP -
P8T33_RS26145 (P8T33_26140) 63902..65326 - 1425 WP_023158013 F-type conjugal transfer pilus assembly protein TraB traB
P8T33_RS26150 (P8T33_26145) 65326..66066 - 741 WP_014386193 type-F conjugative transfer system secretin TraK traK
P8T33_RS26155 (P8T33_26150) 66053..66619 - 567 WP_016831035 type IV conjugative transfer system protein TraE traE
P8T33_RS26160 (P8T33_26155) 66639..66944 - 306 WP_004144424 type IV conjugative transfer system protein TraL traL
P8T33_RS26165 (P8T33_26160) 66958..67326 - 369 WP_004194426 type IV conjugative transfer system pilin TraA -
P8T33_RS26170 (P8T33_26165) 67388..67594 - 207 WP_171773970 TraY domain-containing protein -
P8T33_RS26175 (P8T33_26170) 67727..68446 - 720 WP_016831034 conjugal transfer protein TrbJ -
P8T33_RS26180 (P8T33_26175) 68646..69062 - 417 WP_278030427 conjugal transfer relaxosome DNA-binding protein TraM -
P8T33_RS26185 (P8T33_26180) 69453..69938 + 486 WP_004178063 transglycosylase SLT domain-containing protein virB1
P8T33_RS26190 (P8T33_26185) 69971..70300 - 330 WP_011977736 DUF5983 family protein -
P8T33_RS26195 (P8T33_26190) 70333..71154 - 822 WP_004182076 DUF932 domain-containing protein -
P8T33_RS26200 (P8T33_26195) 71982..72545 - 564 WP_004182054 methyltransferase -
P8T33_RS26205 (P8T33_26200) 72593..73948 - 1356 WP_004182052 DUF3560 domain-containing protein -
P8T33_RS26210 (P8T33_26205) 74000..74230 - 231 WP_001568051 hypothetical protein -
P8T33_RS26215 (P8T33_26210) 74322..74549 - 228 WP_004182050 hypothetical protein -

Host bacterium

ID   6889 GenBank   NZ_CP121144
Plasmid name   pESBL-PH-57 Incompatibility group   IncFII
Plasmid size   247179 bp Coordinate of oriT [Strand]   69331..69380 [+]
Host baterium   Klebsiella pneumoniae strain UHD-57_PH

Cargo genes

Drug resistance gene   qnrB1, dfrA14, sul2, aph(3'')-Ib, aph(6)-Id, blaTEM-1B, blaCTX-M-15, aac(3)-IIa, blaOXA-1, aac(6')-Ib-cr
Virulence gene   -
Metal resistance gene   silE, silS, silR, silC, silF, silB, silA, silP, pcoA, pcoB, pcoC, pcoD, pcoR, pcoS, pcoE, arsH, arsC, arsB, arsA, arsD, arsR, fecD, fecE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -