Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106450
Name   oriT_pESBL-PH-49 in_silico
Organism   Klebsiella pneumoniae strain UHD-49_PH
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP121130 (191321..191370 [-], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_pESBL-PH-49
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4299 GenBank   WP_015065542
Name   traI_P8T37_RS26935_pESBL-PH-49 insolico UniProt ID   K7R3U7
Length   1752 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1752 a.a.        Molecular weight: 190318.03 Da        Isoelectric Point: 6.2890

>WP_015065542.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacterales]
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMSREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALAVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAAGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDTAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGGGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD

  Protein domains


Predicted by InterproScan.

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(576-628)

DNA helicase TraI (TraI_C)

(1432-1554)

TrwC relaxase (TrwC)

(11-283)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(634-713)

AAA domain (AAA_30)

(970-1156)


  Protein structure



No available structure.




T4CP

ID   4328 GenBank   WP_015065541
Name   traD_P8T37_RS26930_pESBL-PH-49 insolico UniProt ID   _
Length   770 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 770 a.a.        Molecular weight: 86025.00 Da        Isoelectric Point: 5.0577

>WP_015065541.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 190763..220777

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P8T37_RS26710 (P8T37_26710) 186152..186379 + 228 WP_004182050 hypothetical protein -
P8T37_RS26715 (P8T37_26715) 186471..186701 + 231 WP_001568051 hypothetical protein -
P8T37_RS26720 (P8T37_26720) 186753..188108 + 1356 WP_004182052 DUF3560 domain-containing protein -
P8T37_RS26725 (P8T37_26725) 188156..188719 + 564 WP_004182054 methyltransferase -
P8T37_RS26730 (P8T37_26730) 189547..190368 + 822 WP_004182076 DUF932 domain-containing protein -
P8T37_RS26735 (P8T37_26735) 190401..190730 + 330 WP_011977736 DUF5983 family protein -
P8T37_RS26740 (P8T37_26740) 190763..191248 - 486 WP_004178063 transglycosylase SLT domain-containing protein virB1
P8T37_RS26745 (P8T37_26745) 191639..192055 + 417 WP_278030427 conjugal transfer relaxosome DNA-binding protein TraM -
P8T37_RS26750 (P8T37_26750) 192255..192974 + 720 WP_016831034 conjugal transfer protein TrbJ -
P8T37_RS26755 (P8T37_26755) 193107..193313 + 207 WP_171773970 TraY domain-containing protein -
P8T37_RS26760 (P8T37_26760) 193375..193743 + 369 WP_004194426 type IV conjugative transfer system pilin TraA -
P8T37_RS26765 (P8T37_26765) 193757..194062 + 306 WP_004144424 type IV conjugative transfer system protein TraL traL
P8T37_RS26770 (P8T37_26770) 194082..194648 + 567 WP_016831035 type IV conjugative transfer system protein TraE traE
P8T37_RS26775 (P8T37_26775) 194635..195375 + 741 WP_014386193 type-F conjugative transfer system secretin TraK traK
P8T37_RS26780 (P8T37_26780) 195375..196799 + 1425 WP_023158013 F-type conjugal transfer pilus assembly protein TraB traB
P8T37_RS26785 (P8T37_26785) 196792..197388 + 597 Protein_204 conjugal transfer pilus-stabilizing protein TraP -
P8T37_RS26790 (P8T37_26790) 197411..197995 + 585 WP_014386196 type IV conjugative transfer system lipoprotein TraV traV
P8T37_RS26795 (P8T37_26795) 198127..198537 + 411 WP_016831039 hypothetical protein -
P8T37_RS26800 (P8T37_26800) 198542..198831 + 290 Protein_207 hypothetical protein -
P8T37_RS26805 (P8T37_26805) 198855..199073 + 219 WP_014386199 hypothetical protein -
P8T37_RS26810 (P8T37_26810) 199074..199385 + 312 WP_014386200 hypothetical protein -
P8T37_RS26815 (P8T37_26815) 199452..199856 + 405 WP_016831042 hypothetical protein -
P8T37_RS26820 (P8T37_26820) 199899..200288 + 390 WP_023158009 hypothetical protein -
P8T37_RS26825 (P8T37_26825) 200296..200694 + 399 WP_023158008 hypothetical protein -
P8T37_RS26830 (P8T37_26830) 200766..203405 + 2640 WP_023158007 type IV secretion system protein TraC virb4
P8T37_RS26835 (P8T37_26835) 203405..203794 + 390 WP_004167468 type-F conjugative transfer system protein TrbI -
P8T37_RS26840 (P8T37_26840) 203794..204429 + 636 WP_023158006 type-F conjugative transfer system protein TraW traW
P8T37_RS26845 (P8T37_26845) 204464..204865 + 402 WP_023158005 hypothetical protein -
P8T37_RS26850 (P8T37_26850) 204862..205851 + 990 WP_015632509 conjugal transfer pilus assembly protein TraU traU
P8T37_RS26855 (P8T37_26855) 205872..206048 + 177 WP_162866177 hypothetical protein -
P8T37_RS26860 (P8T37_26860) 206127..206774 + 648 WP_015632510 type-F conjugative transfer system pilin assembly protein TrbC trbC
P8T37_RS26865 (P8T37_26865) 206833..208788 + 1956 WP_015065533 type-F conjugative transfer system mating-pair stabilization protein TraN traN
P8T37_RS26870 (P8T37_26870) 208820..209074 + 255 WP_015065558 conjugal transfer protein TrbE -
P8T37_RS26875 (P8T37_26875) 209052..209300 + 249 WP_004152675 hypothetical protein -
P8T37_RS26880 (P8T37_26880) 209313..209639 + 327 WP_015065534 hypothetical protein -
P8T37_RS26885 (P8T37_26885) 209660..210412 + 753 WP_015065535 type-F conjugative transfer system pilin assembly protein TraF traF
P8T37_RS26890 (P8T37_26890) 210423..210662 + 240 WP_023158003 type-F conjugative transfer system pilin chaperone TraQ -
P8T37_RS26895 (P8T37_26895) 210634..211191 + 558 WP_023158002 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
P8T37_RS26900 (P8T37_26900) 211237..211680 + 444 WP_023158001 F-type conjugal transfer protein TrbF -
P8T37_RS26905 (P8T37_26905) 211658..213037 + 1380 WP_023158000 conjugal transfer pilus assembly protein TraH traH
P8T37_RS26910 (P8T37_26910) 213037..215880 + 2844 WP_023157999 conjugal transfer mating-pair stabilization protein TraG traG
P8T37_RS26915 (P8T37_26915) 215891..216415 + 525 WP_015065538 hypothetical protein -
P8T37_RS26920 (P8T37_26920) 216724..217455 + 732 WP_032329926 conjugal transfer complement resistance protein TraT -
P8T37_RS26925 (P8T37_26925) 217647..218336 + 690 WP_015065540 hypothetical protein -
P8T37_RS26930 (P8T37_26930) 218465..220777 + 2313 WP_015065541 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   6887 GenBank   NZ_CP121130
Plasmid name   pESBL-PH-49 Incompatibility group   IncFIB
Plasmid size   247178 bp Coordinate of oriT [Strand]   191321..191370 [-]
Host baterium   Klebsiella pneumoniae strain UHD-49_PH

Cargo genes

Drug resistance gene   dfrA14, qnrB1, tet(A), aac(6')-Ib-cr, blaOXA-1, aac(3)-IIa, blaCTX-M-15, blaTEM-1B, aph(6)-Id, aph(3'')-Ib, sul2
Virulence gene   -
Metal resistance gene   fecE, fecD, arsR, arsD, arsA, arsB, arsC, arsH, pcoE, pcoS, pcoR, pcoD, pcoC, pcoB, pcoA, silP, silA, silB, silF, silC, silR, silS, silE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -