Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106437
Name   oriT_pKO_5 in_silico
Organism   Klebsiella michiganensis strain KO_408
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP091475 (8363..8407 [+], 45 nt)
oriT length   45 nt
IRs (inverted repeats)      7..13, 25..31  (CGTTTCT..AGAAACG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 45 nt

>oriT_pKO_5
GCGCACCGTTTCTGAACGAAGTGAAGAAACGTCTAAGTGCGCCCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4293 GenBank   WP_016946648
Name   mobA_L3249_RS30900_pKO_5 insolico UniProt ID   A0A500IE68
Length   371 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 371 a.a.        Molecular weight: 43186.77 Da        Isoelectric Point: 6.4038

>WP_016946648.1 MULTISPECIES: mobilization protein MobA [Gammaproteobacteria]
MASYHLSVKTGGKGSASPHADYIAREGKYAREKDSDLEHKESGNMPAWAAHKPSEFWKAADTSERANGCT
YREIEIALPRELKPEQRLELVRDFVQQEIGDRHAYQFAIHNPKAAIAGGEQPHAHIMFSERINDGIHRDP
EQYFKRANTKEPERGGAKKARFGETPTERKEYLVAQRERWADLQNKHLERYQHADRVDSRSLKAQGIDRE
PERHLGAGQVQRFDTEQLQAILERREAERQVQQSRDERDSVIDVTTSLREALSERDTLTLKQELKSEPEQ
ESHSGRTFDFEKEPDKLNALVSDAMKDIQEEIDLQSLVNDAMAEFQGIHQEMERQRERERLAEKQRQQEK
ERQRLAEQIRQKPDKGWSFSR

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(42-218)


  Protein structure


Source ID Structure
AlphaFold DB A0A500IE68


Host bacterium

ID   6874 GenBank   NZ_CP091475
Plasmid name   pKO_5 Incompatibility group   ColE10
Plasmid size   9564 bp Coordinate of oriT [Strand]   8363..8407 [+]
Host baterium   Klebsiella michiganensis strain KO_408

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -