Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106398
Name   oriT_p20ES-184 in_silico
Organism   Enterobacter cloacae strain 20ES
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CM008910 (28873..29023 [-], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      12..17, 24..29  (AACCCG..CGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_p20ES-184
TGGTCGATGTGAACCCGTTTGACCGGGTTATGAATGAGTTGAAAAGTCGTGGCCGCAAGAACGCTCATATCCTGAGCATCCTTCAATTCGACTGGCCCGCATCGGAGGTCATCATCGAGAAGCTGAGCTGTTACATCACAGATGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4282 GenBank   WP_185846507
Name   mobH_CS906_RS02275_p20ES-184 insolico UniProt ID   _
Length   987 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 987 a.a.        Molecular weight: 109445.08 Da        Isoelectric Point: 4.9176

>WP_185846507.1 MobH family relaxase [Enterobacter cloacae]
MLKALHKLFGGRSGAIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWATQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAYRHGIDRYFIRWRDKRHKRHEQFSL
LAWHRIIPAETQEFLSKSGPSIIEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVNEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISQDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGPVKILMLRLESNDLVFTTEPPAAVTGEVVGDVEDAEIE
FVDPEEADEDQAEGDAGLNNDMLAAELEAEKALAGLGFGDAMAMLKSTSDIAEEKPEQEDTESTELPKPD
ASKKGKQQSKPGKARAEKQPQKPEVKEDLSPQDIAKNAPPLANDDPLQALKDVGGGLGDIDFPFDVFNAS
AETACTDATNLEIPDITEPEKQEEQPKPDFVPQEQNSLQNGDFPIFSDSDEPPSWAIEPLPMLTDTPEQT
IPAPDMQHAGKPKELEKDAKTLLSEMLAGYGEASTLLEQAIMPVLEGKTTLGEVLCLMKGQAVILYPEGA
RSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLVDQLSDAVVAAIKDAEASMGGYQEAFELV
SPPGSGVSKNKSAPKQQSRKKTQQQKPEVDAGAGAEQKTKDKSLQRQPKEKQGNVASLVEEQKRKPVQDQ
EKQNVARLPKREAQPVAPKPKVEHERELGHVEVRERDEPEVREFEPPKVKTNPKDINEEDFLPSGVTPEK
ALLMLKDMIQKRSGRWLVTPVLEEDGCLVTSVKAFDMIAGENVGISKHILYGLLSRAQRRPLLKKRQGKL
YLEVDKA

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4CP

ID   4301 GenBank   WP_012766329
Name   traC_CS906_RS02195_p20ES-184 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92529.82 Da        Isoelectric Point: 5.8328

>WP_012766329.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIITIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDQSVGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTSLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTDRILAKTSKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQYLAQIRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDREAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYDDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSQKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSTFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSDGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRKQAA

  Protein domains


Predicted by InterproScan.

TraC protein (TraC_F_IV)

(21-255)

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

  Protein structure



No available structure.



ID   4302 GenBank   WP_086598693
Name   traD_CS906_RS02270_p20ES-184 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69234.73 Da        Isoelectric Point: 7.1093

>WP_086598693.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacter cloacae complex]
MTMSYDPLAYEMPWRPNYEKNAVAGWMAASCAALAVEQVSTMPSEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPEDMWLGSGFLWENRHAQRVFEILKRDWTSIVGKESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVLTHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVKPDWEAEAGAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNTDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-293)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 5835..30939

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CS906_RS02135 (CS906_04570) 962..1867 - 906 WP_185846503 hypothetical protein -
CS906_RS02140 (CS906_04575) 1879..2847 - 969 WP_001553257 AAA family ATPase -
CS906_RS02145 (CS906_04580) 3067..3294 - 228 WP_236732500 hypothetical protein -
CS906_RS02150 (CS906_04585) 3563..3883 - 321 WP_012766334 hypothetical protein -
CS906_RS02155 (CS906_04590) 4174..4824 + 651 WP_000796641 hypothetical protein -
CS906_RS02160 (CS906_04595) 4936..5796 + 861 WP_012766333 hypothetical protein -
CS906_RS02165 (CS906_04600) 5835..8573 - 2739 WP_185846504 conjugal transfer mating pair stabilization protein TraN traN
CS906_RS02170 (CS906_04605) 8724..9728 - 1005 WP_000911304 TraU family protein traU
CS906_RS02175 (CS906_04610) 9722..10399 - 678 WP_012766331 EAL domain-containing protein -
CS906_RS02180 (CS906_04615) 10396..11664 - 1269 WP_001553264 TrbC family F-type conjugative pilus assembly protein traW
CS906_RS02185 (CS906_04620) 11627..12157 - 531 WP_000672456 S26 family signal peptidase -
CS906_RS02190 (CS906_04625) 12154..12471 - 318 WP_000364398 hypothetical protein -
CS906_RS02195 (CS906_04630) 12486..14933 - 2448 WP_012766329 type IV secretion system protein TraC virb4
CS906_RS02200 (CS906_04635) 14930..15637 - 708 WP_001259344 DsbC family protein -
CS906_RS02205 (CS906_04640) 15786..21272 - 5487 WP_185846505 Ig-like domain-containing protein -
CS906_RS02210 (CS906_04645) 21473..21865 - 393 WP_012766327 TraA family conjugative transfer protein -
CS906_RS02215 (CS906_04650) 21869..22447 - 579 WP_000794193 type IV conjugative transfer system lipoprotein TraV traV
CS906_RS02220 (CS906_04655) 22444..23760 - 1317 WP_185846506 TraB/VirB10 family protein traB
CS906_RS02225 (CS906_04660) 23757..24674 - 918 WP_012766326 type-F conjugative transfer system secretin TraK traK
CS906_RS02230 (CS906_04665) 24658..25284 - 627 WP_001049718 TraE/TraK family type IV conjugative transfer system protein traE
CS906_RS02235 (CS906_04670) 25281..25562 - 282 WP_000805626 type IV conjugative transfer system protein TraL traL
CS906_RS02240 (CS906_04675) 25705..26052 - 348 WP_001102160 hypothetical protein -
CS906_RS27920 26065..26208 - 144 WP_023356351 hypothetical protein -
CS906_RS02245 (CS906_04680) 26387..27049 - 663 WP_000045163 hypothetical protein -
CS906_RS02250 (CS906_04685) 27049..27426 - 378 WP_000869260 hypothetical protein -
CS906_RS02255 (CS906_04690) 27436..27882 - 447 WP_000122583 hypothetical protein -
CS906_RS02260 (CS906_04695) 27893..28522 - 630 WP_000743448 DUF4400 domain-containing protein tfc7
CS906_RS02265 (CS906_04700) 28479..29024 - 546 WP_023356350 hypothetical protein -
CS906_RS02270 (CS906_04705) 29074..30939 - 1866 WP_086598693 conjugative transfer system coupling protein TraD virb4
CS906_RS02275 (CS906_04710) 30936..33899 - 2964 WP_185846507 MobH family relaxase -
CS906_RS02280 (CS906_04715) 34067..34684 + 618 WP_012766323 hypothetical protein -
CS906_RS02285 (CS906_04720) 34666..34899 + 234 WP_001191893 hypothetical protein -
CS906_RS02290 (CS906_04725) 34899..35423 + 525 Protein_33 toprim domain-containing protein -

Host bacterium

ID   6835 GenBank   NZ_CM008910
Plasmid name   p20ES-184 Incompatibility group   IncA/C
Plasmid size   178976 bp Coordinate of oriT [Strand]   28873..29023 [-]
Host baterium   Enterobacter cloacae strain 20ES

Cargo genes

Drug resistance gene   aph(3'')-Ib, cmlB1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -