Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106397
Name   oriT_p20ES-288 in_silico
Organism   Enterobacter cloacae strain 20ES
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CM008909 (78477..78731 [-], 255 nt)
oriT length   255 nt
IRs (inverted repeats)      153..158, 160..165  (AAAAGT..ACTTTT)
 122..130, 135..143  (TTAAGGCTT..AAGCCTTAA)
 49..55, 60..66  (GAATTTT..AAAATTC)
Location of nic site      78..79
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 255 nt

>oriT_p20ES-288
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4281 GenBank   WP_242449839
Name   mobH_CS906_RS01015_p20ES-288 insolico UniProt ID   _
Length   718 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 718 a.a.        Molecular weight: 81943.68 Da        Isoelectric Point: 4.8737

>WP_242449839.1 MobH family relaxase [Enterobacter cloacae]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVIRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDVITKITDT
VETAVLKVNDLGRSSASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDTTMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLELPPP
TCKCSRRTFWRRGISWNR

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(108-304)


  Protein structure



No available structure.




T4CP

ID   4300 GenBank   WP_001284073
Name   traD_CS906_RS01020_p20ES-288 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 78123.91 Da        Isoelectric Point: 8.0177

>WP_001284073.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(406-609)

AAA-like domain (AAA_10)

(199-249)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 229799..239005

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CS906_RS27910 227089..227250 - 162 WP_001371949 hypothetical protein -
CS906_RS01845 (CS906_04275) 229799..232480 - 2682 WP_185846499 TraC family protein virb4
CS906_RS01850 (CS906_04280) 232489..233439 - 951 WP_001022588 IncHI-type conjugal transfer lipoprotein TrhV traV
CS906_RS01855 (CS906_04285) 233449..234201 - 753 WP_182911723 protein-disulfide isomerase HtdT -
CS906_RS01860 (CS906_04290) 234320..234802 - 483 WP_000377632 hypothetical protein -
CS906_RS01865 (CS906_04295) 234810..236165 - 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
CS906_RS01870 (CS906_04300) 236155..236616 - 462 WP_000521242 plasmid transfer protein HtdO -
CS906_RS01875 (CS906_04305) 236686..237888 - 1203 WP_242449837 type-F conjugative transfer system secretin TraK traK
CS906_RS01880 (CS906_04310) 237888..238676 - 789 WP_185846500 TraE/TraK family type IV conjugative transfer system protein traE
CS906_RS01885 (CS906_04315) 238688..239038 - 351 WP_001447770 type IV conjugative transfer system protein TraL -
CS906_RS01890 (CS906_04320) 239056..239409 - 354 WP_000423602 hypothetical protein -
CS906_RS01900 (CS906_04330) 240558..241433 - 876 WP_000594611 RepB family plasmid replication initiator protein -
CS906_RS01905 (CS906_04335) 241910..242962 + 1053 WP_001065778 hypothetical protein -
CS906_RS01910 (CS906_04340) 242982..243182 + 201 WP_000594930 hypothetical protein -
CS906_RS01915 (CS906_04345) 243179..243895 + 717 WP_000838219 zinc finger-like domain-containing protein -

Host bacterium

ID   6834 GenBank   NZ_CM008909
Plasmid name   p20ES-288 Incompatibility group   IncHI2A
Plasmid size   282693 bp Coordinate of oriT [Strand]   78477..78731 [-]
Host baterium   Enterobacter cloacae strain 20ES

Cargo genes

Drug resistance gene   blaSHV-12, aadA2, qacE, sul1, qnrA1, dfrA19, aph(3'')-Ib, aph(6)-Id, mcr-9, catA2, blaTEM-1B
Virulence gene   -
Metal resistance gene   pcoS, pcoE, ncrC, rcnR/yohL, arsC, arsB, arsR, arsH, merR, merT, merP, merC, merA, merD, merE, pbrA, terE, terD, terC, terB, terA, terZ, terW
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -