Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 106396 |
| Name | oriT_p20ES-93  |
| Organism | Enterobacter cloacae strain 20ES |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CM008908 (45092..45196 [-], 105 nt) |
| oriT length | 105 nt |
| IRs (inverted repeats) | 32..37, 40..45 (GCCGGC..GCCGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 105 nt
>oriT_p20ES-93
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 4280 | GenBank | WP_004206924 |
| Name | Relaxase_CS906_RS00220_p20ES-93 |
UniProt ID | A0A2V4FJ40 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75087.84 Da Isoelectric Point: 10.0285
>WP_004206924.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2V4FJ40 |
T4CP
| ID | 4299 | GenBank | WP_004206885 |
| Name | t4cp2_CS906_RS00470_p20ES-93 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 18143..42294
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CS906_RS00085 (CS906_00085) | 14464..14940 | + | 477 | Protein_16 | Tn3 family transposase | - |
| CS906_RS00090 (CS906_00090) | 15831..16886 | - | 1056 | WP_021526650 | plasmid replication initiator RepA | - |
| CS906_RS00095 (CS906_00095) | 17183..17413 | - | 231 | WP_185846481 | IncL/M type plasmid replication protein RepC | - |
| CS906_RS00100 (CS906_00100) | 17487..18140 | - | 654 | WP_004206935 | hypothetical protein | - |
| CS906_RS00105 (CS906_00105) | 18143..18547 | - | 405 | WP_242449833 | hypothetical protein | traY |
| CS906_RS00110 (CS906_00110) | 18549..21554 | - | 3006 | WP_001143760 | Tn3-like element Tn3 family transposase | - |
| CS906_RS00115 (CS906_00115) | 21718..22275 | + | 558 | WP_001235713 | recombinase family protein | - |
| CS906_RS00120 (CS906_00120) | 22458..23318 | + | 861 | WP_000027057 | broad-spectrum class A beta-lactamase TEM-1 | - |
| CS906_RS00125 (CS906_00125) | 23413..25278 | - | 1866 | WP_242449834 | DotA/TraY family protein | traY |
| CS906_RS00130 (CS906_00130) | 25271..25921 | - | 651 | WP_060450166 | hypothetical protein | - |
| CS906_RS00135 (CS906_00135) | 25918..27126 | - | 1209 | WP_004187486 | conjugal transfer protein TraW | traW |
| CS906_RS00140 (CS906_00140) | 27123..30173 | - | 3051 | WP_011154474 | conjugative transfer protein | traU |
| CS906_RS00145 (CS906_00145) | 30170..30664 | - | 495 | WP_004187480 | hypothetical protein | - |
| CS906_RS00150 (CS906_00150) | 30710..31099 | - | 390 | WP_011154472 | DUF6750 family protein | traR |
| CS906_RS00155 (CS906_00155) | 31116..31646 | - | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| CS906_RS00160 (CS906_00160) | 31670..32374 | - | 705 | WP_004206933 | conjugal transfer protein TraP | traP |
| CS906_RS00165 (CS906_00165) | 32386..33735 | - | 1350 | WP_004206932 | conjugal transfer protein TraO | traO |
| CS906_RS00170 (CS906_00170) | 33747..34898 | - | 1152 | WP_015062843 | DotH/IcmK family type IV secretion protein | traN |
| CS906_RS00175 (CS906_00175) | 34907..35620 | - | 714 | WP_227549829 | DotI/IcmL family type IV secretion protein | traM |
| CS906_RS00180 (CS906_00180) | 35688..36098 | + | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| CS906_RS00185 (CS906_00185) | 36127..36282 | + | 156 | WP_172693602 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| CS906_RS00190 (CS906_00190) | 36283..36795 | - | 513 | WP_011091071 | hypothetical protein | traL |
| CS906_RS28095 | 36761..40066 | - | 3306 | WP_227549828 | LPD7 domain-containing protein | - |
| CS906_RS00200 (CS906_00200) | 40091..40351 | - | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| CS906_RS00205 (CS906_00205) | 40341..41504 | - | 1164 | WP_004206926 | plasmid transfer ATPase TraJ | virB11 |
| CS906_RS00210 (CS906_00210) | 41515..42294 | - | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| CS906_RS00215 (CS906_00215) | 42291..42791 | - | 501 | WP_004206925 | DotD/TraH family lipoprotein | - |
| CS906_RS00220 (CS906_00220) | 42805..44784 | - | 1980 | WP_004206924 | TraI/MobA(P) family conjugative relaxase | - |
| CS906_RS00225 (CS906_00225) | 44771..45088 | - | 318 | WP_004206923 | plasmid mobilization protein MobA | - |
| CS906_RS00230 (CS906_00230) | 45364..45729 | + | 366 | WP_015586046 | hypothetical protein | - |
| CS906_RS00235 (CS906_00235) | 46230..46766 | - | 537 | WP_004206920 | hypothetical protein | - |
| CS906_RS00240 (CS906_00240) | 46899..47210 | - | 312 | WP_004187333 | hypothetical protein | - |
Host bacterium
| ID | 6833 | GenBank | NZ_CM008908 |
| Plasmid name | p20ES-93 | Incompatibility group | IncL/M |
| Plasmid size | 84608 bp | Coordinate of oriT [Strand] | 45092..45196 [-] |
| Host baterium | Enterobacter cloacae strain 20ES |
Cargo genes
| Drug resistance gene | aac(3)-IIa, blaOXA-1, aac(6')-Ib-cr, tet(A), blaTEM-1B, aph(6)-Id, aph(3'')-Ib, blaSCO-1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |