Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106358
Name   oriT_CFSAN002050|unnamed in_silico
Organism   Salmonella enterica subsp. enterica serovar Cubana str. CFSAN002050
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NC_021845 (142384..142638 [+], 255 nt)
oriT length   255 nt
IRs (inverted repeats)      153..158, 160..165  (AAAAGT..ACTTTT)
 122..128, 137..143  (TTAAGGC..GCCTTAA)
 49..55, 60..66  (GAATTTT..AAAATTC)
Location of nic site      78..79
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 255 nt

>oriT_CFSAN002050|unnamed
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCATATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4259 GenBank   WP_242405922
Name   mobH_CFSAN002050_RS24910_CFSAN002050|unnamed insolico UniProt ID   _
Length   1050 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1050 a.a.        Molecular weight: 118137.60 Da        Isoelectric Point: 4.5894

>WP_242405922.1 MobH family relaxase [Salmonella enterica]
MMMNFRALYFCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVTKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKIVTEYRRPVPGRDAITKITDT
VETAVLKVNDLGRSSASIDVDIHSEKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDATMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGQLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFTQLSISRPEVG
SCATSSSVHNEKLLPEPSELPELNREQNADPQGTIERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGYNLNSKVFASTESDQNGEFSLISEGDVTELEFVEIALVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(108-304)


  Protein structure



No available structure.




T4CP

ID   4280 GenBank   WP_001284073
Name   traD_CFSAN002050_RS24905_CFSAN002050|unnamed insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 78123.91 Da        Isoelectric Point: 8.0177

>WP_001284073.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(406-609)

AAA-like domain (AAA_10)

(199-249)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 38418..47625

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFSAN002050_RS24340 (CFSAN002050_00160) 38418..41099 - 2682 WP_000387412 TraC family protein virb4
CFSAN002050_RS24345 (CFSAN002050_00165) 41108..42058 - 951 WP_020833706 IncHI-type conjugal transfer lipoprotein TrhV traV
CFSAN002050_RS24350 (CFSAN002050_00170) 42068..42820 - 753 WP_202196077 protein-disulfide isomerase HtdT -
CFSAN002050_RS24355 (CFSAN002050_00175) 42939..43421 - 483 WP_020833708 hypothetical protein -
CFSAN002050_RS24360 (CFSAN002050_00180) 43429..44784 - 1356 WP_020833709 IncHI-type conjugal transfer protein TrhB traB
CFSAN002050_RS24365 (CFSAN002050_00185) 44774..45235 - 462 WP_000521241 plasmid transfer protein HtdO -
CFSAN002050_RS24370 (CFSAN002050_00190) 45237..46508 - 1272 WP_020833711 type-F conjugative transfer system secretin TraK traK
CFSAN002050_RS24375 (CFSAN002050_00195) 46508..47296 - 789 WP_020833712 TraE/TraK family type IV conjugative transfer system protein traE
CFSAN002050_RS24380 (CFSAN002050_00200) 47308..47625 - 318 WP_000043359 type IV conjugative transfer system protein TraL traL
CFSAN002050_RS24385 (CFSAN002050_00205) 47676..48029 - 354 WP_000423602 hypothetical protein -
CFSAN002050_RS24390 (CFSAN002050_00210) 49181..50056 - 876 WP_000594612 RepB family plasmid replication initiator protein -
CFSAN002050_RS24395 (CFSAN002050_00215) 50533..51585 + 1053 WP_020833714 hypothetical protein -
CFSAN002050_RS24400 (CFSAN002050_00220) 51605..51805 + 201 WP_000594930 hypothetical protein -
CFSAN002050_RS24405 (CFSAN002050_00225) 51802..52518 + 717 WP_020833715 zinc finger-like domain-containing protein -

Host bacterium

ID   6795 GenBank   NC_021845
Plasmid name   CFSAN002050|unnamed Incompatibility group   IncHI2A
Plasmid size   166668 bp Coordinate of oriT [Strand]   142384..142638 [+]
Host baterium   Salmonella enterica subsp. enterica serovar Cubana str. CFSAN002050

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   merE, merD, merA, merC, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -