Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106357
Name   oriT_pSAL65505 in_silico
Organism   Salmonella enterica subsp. enterica serovar Minnesota strain SAL65505
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CM004485 (64662..64750 [+], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pSAL65505
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4257 GenBank   WP_001474686
Name   nikB_A7Y01_RS00315_pSAL65505 insolico UniProt ID   A0A2J0RB70
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103834.33 Da        Isoelectric Point: 7.3473

>WP_001474686.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFLQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A2J0RB70


Auxiliary protein

ID   1892 GenBank   WP_001283947
Name   WP_001283947_pSAL65505 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   4279 GenBank   WP_001289271
Name   trbC_A7Y01_RS00310_pSAL65505 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86927.12 Da        Isoelectric Point: 6.7713

>WP_001289271.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKIDVQELIELH
PGEFFSIFRGETVPSASFFIPDNEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTAQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHLTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 18244..59284

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7Y01_RS23420 13306..14373 + 1068 WP_000742600 type IV pilus biogenesis lipoprotein PilL -
A7Y01_RS00095 (A7Y01_22905) 14373..14810 + 438 WP_000539807 type IV pilus biogenesis protein PilM -
A7Y01_RS00100 (A7Y01_22910) 14824..16506 + 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
A7Y01_RS00105 (A7Y01_22915) 16499..17794 + 1296 WP_000752774 type 4b pilus protein PilO2 -
A7Y01_RS00110 (A7Y01_22920) 17781..18233 + 453 WP_001247336 type IV pilus biogenesis protein PilP -
A7Y01_RS00115 (A7Y01_22925) 18244..19797 + 1554 WP_000362202 ATPase, T2SS/T4P/T4SS family virB11
A7Y01_RS00120 (A7Y01_22930) 19810..20907 + 1098 WP_001208805 type II secretion system F family protein -
A7Y01_RS00125 (A7Y01_22935) 20925..21539 + 615 WP_000959785 type 4 pilus major pilin -
A7Y01_RS00130 (A7Y01_22940) 21549..22109 + 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
A7Y01_RS23425 22094..22750 + 657 WP_001193553 prepilin peptidase -
A7Y01_RS00135 (A7Y01_22945) 22750..24042 + 1293 WP_032274560 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
A7Y01_RS23430 24039..24287 - 249 WP_001349157 hypothetical protein -
A7Y01_RS00150 (A7Y01_22960) 24978..26132 + 1155 WP_001139958 site-specific integrase -
A7Y01_RS00155 (A7Y01_22965) 26283..27107 + 825 WP_001238927 conjugal transfer protein TraE traE
A7Y01_RS00160 (A7Y01_22970) 27193..28395 + 1203 WP_000976353 conjugal transfer protein TraF -
A7Y01_RS26400 28455..28739 + 285 Protein_33 histidine phosphatase family protein -
A7Y01_RS00175 (A7Y01_22985) 30073..30375 + 303 Protein_35 lipopolysaccharide core heptose(II)-phosphate phosphatase -
A7Y01_RS00180 (A7Y01_22990) 30770..31228 + 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
A7Y01_RS00185 (A7Y01_22995) 31225..32043 + 819 WP_000646097 IncI1-type conjugal transfer lipoprotein TraI traI
A7Y01_RS00190 (A7Y01_23000) 32040..33188 + 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
A7Y01_RS23440 33185..33475 + 291 WP_001299214 hypothetical protein traK
A7Y01_RS00195 (A7Y01_23005) 33490..34041 + 552 WP_000014584 phospholipase D family protein -
A7Y01_RS00200 (A7Y01_23010) 34131..37898 + 3768 WP_001141535 LPD7 domain-containing protein -
A7Y01_RS00205 (A7Y01_23015) 37916..38263 + 348 WP_001055900 conjugal transfer protein traL
A7Y01_RS00210 (A7Y01_23020) 38260..38952 + 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
A7Y01_RS00215 (A7Y01_23025) 38963..39946 + 984 WP_001191878 IncI1-type conjugal transfer protein TraN traN
A7Y01_RS00220 (A7Y01_23030) 39949..41238 + 1290 WP_001271997 conjugal transfer protein TraO traO
A7Y01_RS00225 (A7Y01_23035) 41238..41942 + 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
A7Y01_RS00230 (A7Y01_23040) 41942..42469 + 528 WP_001055569 conjugal transfer protein TraQ traQ
A7Y01_RS00235 (A7Y01_23045) 42520..42924 + 405 WP_000086960 IncI1-type conjugal transfer protein TraR traR
A7Y01_RS00240 (A7Y01_23050) 42988..43176 + 189 WP_001277255 putative conjugal transfer protein TraS -
A7Y01_RS00245 (A7Y01_23055) 43160..43960 + 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
A7Y01_RS00250 (A7Y01_23060) 44050..47094 + 3045 WP_001024751 IncI1-type conjugal transfer protein TraU traU
A7Y01_RS23445 47094..47708 + 615 WP_000337394 IncI1-type conjugal transfer protein TraV traV
A7Y01_RS00255 (A7Y01_23065) 47675..48877 + 1203 WP_001189158 IncI1-type conjugal transfer protein TraW traW
A7Y01_RS00260 (A7Y01_23070) 48906..49490 + 585 WP_001037998 conjugal transfer protein TraX -
A7Y01_RS00265 (A7Y01_23075) 49587..51755 + 2169 WP_000698368 DotA/TraY family protein traY
A7Y01_RS00270 (A7Y01_23080) 51826..52488 + 663 WP_000644794 plasmid IncI1-type surface exclusion protein ExcA -
A7Y01_RS00275 (A7Y01_23085) 52560..52769 - 210 WP_000062603 HEAT repeat domain-containing protein -
A7Y01_RS26190 53161..53337 + 177 WP_001054900 hypothetical protein -
A7Y01_RS00280 (A7Y01_23090) 54058..55080 + 1023 WP_001572805 IS21-like element IS100 family transposase -
A7Y01_RS00285 (A7Y01_23095) 55077..55859 + 783 WP_001300609 IS21-like element IS100kyp family helper ATPase IstB -
A7Y01_RS00290 (A7Y01_23100) 56220..56471 + 252 WP_001291965 hypothetical protein -
A7Y01_RS00295 (A7Y01_23105) 56543..56695 - 153 WP_001303307 Hok/Gef family protein -
A7Y01_RS00300 (A7Y01_23110) 56987..58195 + 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
A7Y01_RS00305 (A7Y01_23115) 58214..59284 + 1071 WP_000151575 IncI1-type conjugal transfer protein TrbB trbB
A7Y01_RS00310 (A7Y01_23120) 59277..61568 + 2292 WP_001289271 F-type conjugative transfer protein TrbC -

Host bacterium

ID   6794 GenBank   NZ_CM004485
Plasmid name   pSAL65505 Incompatibility group   IncI1
Plasmid size   102920 bp Coordinate of oriT [Strand]   64662..64750 [+]
Host baterium   Salmonella enterica subsp. enterica serovar Minnesota strain SAL65505

Cargo genes

Drug resistance gene   blaCMY-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -