Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 106317
• Name: oriT_pHMPREF1625_3
• Organism: Staphylococcus aureus 880
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_KK240934 (11180..11324 [-], 145 nt)
• oriT length: 145 nt
• IRs (inverted repeats): 105..110, 114..119 (TTTAAA..TTTAAA) 33..39, 44..50 (TGTCACA..TGTGACA)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 145 nt

>oriT_pHMPREF1625_3
TGTCACAAAAATAAGACAAATTCTACATGTTGTGTCACAAAACTGTGACAAACACAATATATAGTGTCACAAAACTGTGACACTATAGTTTATTGTGGGGTCACTTTAAATTCTTTAAAAACCTTGAAATGGCTGGCTTTGCCAG

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobP2_HMPREF1625_RS14810_pHMPREF1625_3

• ID: 4225
• GenBank: WP_223223918
• Name: mobP2_HMPREF1625_RS14810_pHMPREF1625_3
• UniProt ID: _
• Length: 383 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 383 a.a.
• Molecular weight: 45796.08 Da
• Isoelectric Point: 9.9143

>WP_223223918.1 MULTISPECIES: MobP2 family relaxase [Staphylococcus]
MMPKIVTVSQFEQPNEFYEYSNYVNYMKRGEAQNNKDDFKYDVYAHYMFDEEKSQNMFNEHDNFMSKKAI
NKIKSDFSHAQKNKGMMWKDVISFDNSALESVGIYDSKTHTLDEQKIKEATRKMMKRFEKKEGLEDNLVW
SGAIHYNTDNIHVHVAAVEKVIKRTRGKRKPATLKHMKSTFANELFDIKGERQKINEFIRERIVKGIRDE
SEPCKDKGMKKQIKKVHKLLEDIPRNQWNYNNNILKYIRPEIDKITDIYIKNYHSKDFEVFKNDLKRQTN
LYKETYGENSNYGSYEKTKMKDLYARSGNTILKNIKELDQDLMKVKQQSTHSKSNATITKLKIGKCLNQA
LYRTKYALRSDYDKEKNIQQYYQEFDKQSYRER

Protein domains

Predicted by InterproScan.

• MobL relaxases (MobL) (2-358)

Protein structure

No available structure.

T4CP

t4cp2_HMPREF1625_RS14860_pHMPREF1625_3

• ID: 4244
• GenBank: WP_223873963
• Name: t4cp2_HMPREF1625_RS14860_pHMPREF1625_3
• UniProt ID: _
• Length: 858 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 858 a.a.
• Molecular weight: 98780.14 Da
• Isoelectric Point: 6.3969

>WP_223873963.1 VirD4-like conjugal transfer protein, CD1115 family [Staphylococcus aureus]
MKQLNTKYTDYIFNFRLDAVLLYIPIWVLVFIFFGKKVYQFRQRFKSLNNNEKASGRFATRKEITQQYKA
IPQREHAFEGEGGLPVAMFGVYDFVTKTKQYLEQRKTAIREINGITDLTADEKLELRKDKLGKMNKEFAT
TFFSKREFTFIDESPTNNLIVGTSRSGKGEIVVLSMIENYSRSSEQPSLIVNDMKGELFSASYKTLENRG
YQVEIFNLDQPMESTMSFNPLQQVIDEYMKGDLGEAQEMTKQITYTLTNNEGVEDNEWNLMAGALINAMI
LALCEETLPKNPEKVTLYSVSNMLQTLSTKRFYKEIAIGNKVQLIEVSALDEYMERFPSHSPAKTQYATV
QAAPDKMRSSIIGTALKALQPFTTDTIAKLTSHTSFDINKLGFPSIIDGYATKDSTFELGVQVLRDGENG
IEYEEVEGIQIGVNEYGYWQYPFKTVLKVGDRIETIEKDGNNQVITSYFYADHIDDKGQVTFNQKGELDN
SDITIRKFNSFPKPIAVFMVVPDYNNANHGIASILVNQIVFEISKNSQLYTENQSTYRRVIFHLDEAGNM
PPIPNLSQKVNVSLSRGLRFNFFVQAFSQFKDKYGDAYDAIMDACQNKVYIMATQEQTLKDFSAMIGSQQ
ITVHSRSGEAGAIKSSITENQEERPLLRPDELARLQEGETVVVRNLKRQDKKRNKVMSYPIFNDGKYAMK
YRYQYLSDLMDTSQSIIHFRPMLKARCEHRHLQLEATLVDWDKRIEEMQEGIDGQKEQENKNINDINQYK
QSNEEGNVAVRVKRLETLKEKVGDAIFDKITRRFERYLRTYAEQGKNVNTITQKKLEELVSADNEVKEEE
KTKRIEMIQKFIKEAKTT

Protein domains

Predicted by InterproScan.

• TraM recognition site of TraD and TraG (TraG-D_C) (549-666)

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (143-392)

Protein structure

No available structure.

Host bacterium

• ID: 6754
• GenBank: NZ_KK240934
• Plasmid name: pHMPREF1625_3
• Incompatibility group: -
• Plasmid size: 55706 bp
• Coordinate of oriT [Strand]: 11180..11324 [-]
• Host baterium: Staphylococcus aureus 880

Cargo genes

• Drug resistance gene: VanHAX, aac(6')-aph(2'')
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21, AcrIIA14