Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 106311 |
| Name | oriT_T11|unnamed  |
| Organism | Enterococcus faecalis T11 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_GG688649 (27875..27924 [-], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | 34..35 |
| Conserved sequence flanking the nic site |
GCTTGCAAAA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_T11|unnamed
AATATCGCAACATGGTACCATGTTGCTCTGCTTGCAAAAAGAAAGCCTAC
AATATCGCAACATGGTACCATGTTGCTCTGCTTGCAAAAAGAAAGCCTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 4221 | GenBank | WP_002395042 |
| Name | Relaxase_EFMG_RS16460_T11|unnamed |
UniProt ID | _ |
| Length | 561 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 561 a.a. Molecular weight: 65352.91 Da Isoelectric Point: 6.3222
>WP_002395042.1 relaxase/mobilization nuclease domain-containing protein [Enterococcus faecalis]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKRVFEQISDKIAAKEGAKIIEKSPKQT
HTKYTKWQTESIYKQKIKSRLDFLLEQSSSINDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIIERLKENKNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYG
YGNSGKLFVGGYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVM
QTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSNLLLENELQGDPEL
IQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQSLLENKLERTRNEINQLHEIQAVQKKETTNQNNVKPK
L
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKRVFEQISDKIAAKEGAKIIEKSPKQT
HTKYTKWQTESIYKQKIKSRLDFLLEQSSSINDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIIERLKENKNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYG
YGNSGKLFVGGYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVM
QTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSNLLLENELQGDPEL
IQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQSLLENKLERTRNEINQLHEIQAVQKKETTNQNNVKPK
L
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 1881 | GenBank | WP_002395043 |
| Name | WP_002395043_T11|unnamed |
UniProt ID | _ |
| Length | 118 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 118 a.a. Molecular weight: 14032.06 Da Isoelectric Point: 9.9723
>WP_002395043.1 plasmid mobilization relaxosome protein MobC [Enterococcus faecalis]
MENKQKLKRPIQRKIRFSKEENELINRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTNEIHKIGI
NINQMARLANQFNEISSEDIKDLTDKVQSLNALVQSELNKLIKRKEQS
MENKQKLKRPIQRKIRFSKEENELINRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTNEIHKIGI
NINQMARLANQFNEISSEDIKDLTDKVQSLNALVQSELNKLIKRKEQS
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 4243 | GenBank | WP_002425085 |
| Name | t4cp2_EFMG_RS16480_T11|unnamed |
UniProt ID | _ |
| Length | 609 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 609 a.a. Molecular weight: 70145.14 Da Isoelectric Point: 6.1811
>WP_002425085.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEGEKTTGEDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEGEKTTGEDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 30569..49308
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EFMG_RS16460 (EFMG_03035) | 25766..27451 | - | 1686 | WP_002395042 | relaxase/mobilization nuclease domain-containing protein | - |
| EFMG_RS16465 (EFMG_03036) | 27452..27808 | - | 357 | WP_002395043 | plasmid mobilization relaxosome protein MobC | - |
| EFMG_RS16470 (EFMG_03037) | 28054..28326 | - | 273 | WP_002395044 | LtrD | - |
| EFMG_RS16475 (EFMG_03038) | 28359..30518 | - | 2160 | WP_002425084 | ArdC-like ssDNA-binding domain-containing protein | - |
| EFMG_RS16480 (EFMG_03039) | 30569..32398 | - | 1830 | WP_002425085 | VirD4-like conjugal transfer protein, CD1115 family | virb4 |
| EFMG_RS16485 (EFMG_03040) | 32398..32883 | - | 486 | WP_025187431 | DUF3801 domain-containing protein | gbs1365 |
| EFMG_RS16490 (EFMG_03041) | 32912..33109 | - | 198 | WP_002425087 | hypothetical protein | - |
| EFMG_RS16495 (EFMG_03042) | 33155..33613 | - | 459 | WP_002425088 | single-stranded DNA-binding protein | - |
| EFMG_RS16500 (EFMG_03043) | 33630..34385 | - | 756 | WP_002425089 | Fic family protein | - |
| EFMG_RS16505 (EFMG_03044) | 34498..35106 | - | 609 | WP_002367686 | hypothetical protein | - |
| EFMG_RS16510 (EFMG_03045) | 35090..35335 | - | 246 | WP_002393743 | hypothetical protein | - |
| EFMG_RS16515 (EFMG_03046) | 35322..35948 | - | 627 | WP_002425090 | hypothetical protein | prgL |
| EFMG_RS16520 (EFMG_03047) | 35972..38587 | - | 2616 | WP_002410288 | phage tail tip lysozyme | prgK |
| EFMG_RS16525 (EFMG_03048) | 38599..40989 | - | 2391 | WP_002386486 | VirB4-like conjugal transfer ATPase, CD1110 family | virb4 |
| EFMG_RS16530 (EFMG_03049) | 40943..41296 | - | 354 | WP_002386485 | PrgI family protein | prgIc |
| EFMG_RS16535 (EFMG_03050) | 41298..42098 | - | 801 | WP_002425091 | type IV secretion system protein | prgHb |
| EFMG_RS16540 (EFMG_03051) | 42113..42409 | - | 297 | WP_002382798 | hypothetical protein | - |
| EFMG_RS16545 (EFMG_03052) | 42422..42649 | - | 228 | WP_002365858 | hypothetical protein | - |
| EFMG_RS16550 (EFMG_03053) | 42696..43130 | - | 435 | WP_002365857 | hypothetical protein | - |
| EFMG_RS16555 (EFMG_03054) | 43150..44040 | - | 891 | WP_241761406 | type III secretion system protein PrgD | - |
| EFMG_RS16560 (EFMG_03055) | 44091..44948 | - | 858 | WP_002367673 | pheromone response LPXTG-anchored adhesin PrgC | prgC |
| EFMG_RS16565 (EFMG_03056) | 44976..45329 | - | 354 | WP_002367913 | pheromone response system RNA-binding regulator PrgU | - |
| EFMG_RS16570 (EFMG_03057) | 45391..49308 | - | 3918 | WP_002425093 | LPXTG-anchored aggregation substance PrgB/Asc10 | prgB |
| EFMG_RS16575 (EFMG_03058) | 50801..51190 | - | 390 | WP_002425094 | hypothetical protein | - |
| EFMG_RS16580 (EFMG_03059) | 51464..51658 | - | 195 | WP_002425095 | hypothetical protein | - |
| EFMG_RS16585 (EFMG_03060) | 52118..52396 | - | 279 | WP_002425096 | hypothetical protein | - |
Host bacterium
| ID | 6748 | GenBank | NZ_GG688649 |
| Plasmid name | T11|unnamed | Incompatibility group | - |
| Plasmid size | 64654 bp | Coordinate of oriT [Strand] | 27875..27924 [-] |
| Host baterium | Enterococcus faecalis T11 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | prgB/asc10 |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA9 |