Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 106310 |
| Name | oriT_HIP11704|unnamed  |
| Organism | Enterococcus faecalis HIP11704 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_GG692659 (19148..19177 [+], 30 nt) |
| oriT length | 30 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 30 nt
>oriT_HIP11704|unnamed
AACGTAAGTTAGTGGGTATAAGTGCGCCCT
AACGTAAGTTAGTGGGTATAAGTGCGCCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 4220 | GenBank | WP_000974115 |
| Name | mobQ_EFHG_RS21540_HIP11704|unnamed |
UniProt ID | A0A7H0FKC2 |
| Length | 661 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 661 a.a. Molecular weight: 77412.46 Da Isoelectric Point: 9.3565
>WP_000974115.1 MULTISPECIES: MobQ family relaxase [Bacilli]
MAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDRQTL
WNKMELAEKSPNAQLCREVNVALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHIMLT
MREVDSEGNILNKRKRIPKLDENGNQIFNEKGQRVTVSIKTNDWDRKSLVSEIRKDWADKVNQYLKDRNI
DQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDVLSELDKLENQEKVLKQDQN
FTLKFEKTFSPLEKKELKNLSKELKIFVNDENIDKRISELKRWENSLIFNNKMEIQKQRLMLSKISSERD
MLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVVEKEESKK
IFKEKPFQTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDS
VIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKHID
ELSPLAKHNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSG
NQLQKKRNLDKLIKQTKAKENQSLQRNIPLR
MAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDRQTL
WNKMELAEKSPNAQLCREVNVALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHIMLT
MREVDSEGNILNKRKRIPKLDENGNQIFNEKGQRVTVSIKTNDWDRKSLVSEIRKDWADKVNQYLKDRNI
DQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDVLSELDKLENQEKVLKQDQN
FTLKFEKTFSPLEKKELKNLSKELKIFVNDENIDKRISELKRWENSLIFNNKMEIQKQRLMLSKISSERD
MLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVVEKEESKK
IFKEKPFQTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDS
VIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKHID
ELSPLAKHNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSG
NQLQKKRNLDKLIKQTKAKENQSLQRNIPLR
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7H0FKC2 |
T4CP
| ID | 4242 | GenBank | WP_000096577 |
| Name | t4cp2_EFHG_RS21495_HIP11704|unnamed |
UniProt ID | _ |
| Length | 551 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 551 a.a. Molecular weight: 63042.12 Da Isoelectric Point: 4.7709
>WP_000096577.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Bacilli]
MSSLLAESDGLILGKFQSGNVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLSLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVSEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQDYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVKEEAEEKELLMSDILSSFDEGIEENTANNDNSELPF
MSSLLAESDGLILGKFQSGNVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLSLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVSEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQDYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVKEEAEEKELLMSDILSSFDEGIEENTANNDNSELPF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 4515..16682
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EFHG_RS21450 (EFHG_05001) | 1..613 | + | 613 | WP_002371371 | recombinase family protein | - |
| EFHG_RS21455 (EFHG_05002) | 613..975 | + | 363 | WP_000108744 | toprim domain-containing protein | - |
| EFHG_RS21460 (EFHG_05003) | 1512..3002 | - | 1491 | WP_001025005 | primase C-terminal domain-containing protein | - |
| EFHG_RS21465 (EFHG_05004) | 3406..3654 | - | 249 | WP_000362415 | helix-turn-helix transcriptional regulator | - |
| EFHG_RS22590 | 4092..4196 | + | 105 | WP_113792438 | type I toxin-antitoxin system Fst family toxin | - |
| EFHG_RS22705 (EFHG_05005) | 4515..5219 | - | 705 | WP_002371373 | LPXTG cell wall anchor domain-containing protein | prgC |
| EFHG_RS21475 (EFHG_05006) | 5279..5647 | - | 369 | WP_000516429 | hypothetical protein | - |
| EFHG_RS21480 (EFHG_05007) | 5676..6644 | - | 969 | WP_000122915 | hypothetical protein | - |
| EFHG_RS21485 (EFHG_05008) | 6661..7593 | - | 933 | WP_000382494 | conjugal transfer protein TrbL family protein | trsL |
| EFHG_RS21490 (EFHG_05009) | 7595..8518 | - | 924 | WP_001013727 | hypothetical protein | traP |
| EFHG_RS21495 (EFHG_05010) | 8536..10191 | - | 1656 | WP_000096577 | VirD4-like conjugal transfer protein, CD1115 family | - |
| EFHG_RS21500 (EFHG_05011) | 10184..10615 | - | 432 | WP_001085135 | hypothetical protein | - |
| EFHG_RS21505 (EFHG_05012) | 10620..11171 | - | 552 | WP_000393725 | hypothetical protein | - |
| EFHG_RS21510 (EFHG_05013) | 11184..12293 | - | 1110 | WP_000501303 | lysozyme family protein | orf14 |
| EFHG_RS21515 (EFHG_05014) | 12315..13667 | - | 1353 | WP_000875579 | hypothetical protein | trsF |
| EFHG_RS21520 (EFHG_05015) | 13681..15642 | - | 1962 | WP_000411529 | hypothetical protein | virb4 |
| EFHG_RS21525 (EFHG_05016) | 15653..16282 | - | 630 | WP_000459258 | hypothetical protein | - |
| EFHG_RS21530 (EFHG_05017) | 16299..16682 | - | 384 | WP_001083514 | glycosyl transferase | trsC |
| EFHG_RS21535 (EFHG_05018) | 16701..17033 | - | 333 | WP_000713503 | CagC family type IV secretion system protein | - |
| EFHG_RS21540 (EFHG_05019) | 17057..19042 | - | 1986 | WP_000974115 | MobQ family relaxase | - |
| EFHG_RS21545 (EFHG_05020) | 19334..19633 | + | 300 | WP_000431202 | hypothetical protein | - |
| EFHG_RS21550 (EFHG_05021) | 19636..19893 | + | 258 | WP_000002668 | hypothetical protein | - |
| EFHG_RS21555 (EFHG_05022) | 19916..20221 | - | 306 | WP_000351790 | hypothetical protein | - |
| EFHG_RS21560 (EFHG_05023) | 20255..20752 | - | 498 | WP_000874092 | DnaJ domain-containing protein | - |
| EFHG_RS21565 (EFHG_05024) | 20791..21108 | - | 318 | WP_000415964 | hypothetical protein | - |
Host bacterium
| ID | 6747 | GenBank | NZ_GG692659 |
| Plasmid name | HIP11704|unnamed | Incompatibility group | - |
| Plasmid size | 44912 bp | Coordinate of oriT [Strand] | 19148..19177 [+] |
| Host baterium | Enterococcus faecalis HIP11704 |
Cargo genes
| Drug resistance gene | VanHAX |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |