Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106309
Name   oriT_1,231,410|unnamed in_silico
Organism   Enterococcus faecium 1231410
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_GG692535 (39677..39868 [-], 192 nt)
oriT length   192 nt
IRs (inverted repeats)      106..112, 122..128  (ATTTTTT..AAAAAAT)
 107..113, 120..126  (TTTTTTG..CAAAAAA)
 93..98, 105..110  (AAAATA..TATTTT)
 65..71, 75..81  (AGTTGGC..GCCAACT)
 41..49, 52..60  (CACCTTCCT..AGGAAGGTG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 192 nt

>oriT_1,231,410|unnamed
CCAAGGAATTAATACAAAGAGCATAAGGGAAAACTGATAGCACCTTCCTAAAGGAAGGTGGCTAAGTTGGCTGTGCCAACTGGTTTTCTTTCAAAATAGCTTCATATTTTTTGCTATCACAAAAAAATCCATTTTCGACCTATTTTCGGTCATAATATAGTACCTACTTTTGGTCATAGTTTCGTCCGTAGT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4219 GenBank   WP_002326870
Name   mobP2_EFTG_RS20645_1,231,410|unnamed insolico UniProt ID   B5U8V6
Length   506 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 506 a.a.        Molecular weight: 60597.01 Da        Isoelectric Point: 9.3485

>WP_002326870.1 MULTISPECIES: MobP2 family relaxase [Enterococcus]
MTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDYISRQEAIRQDKLDHDYSEDELNELARIEKAL
EKIEQETDQPVIKDLREMDKYIDYMTRKKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNG
SVMFQDVISFDNDFLVREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHV
TAMERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLELKQSVKDSLI
QLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDNPKRKEYDRMTKEIDEL
YQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLLKQSEEKNLKSNELKNLLDGKKLNVKFDREKPEF
KTKYAKQSYDDFQKRLEERQLAREKNEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMI
EQEKMRQAREFEIGMN

  Protein domains


Predicted by InterproScan.

MobL relaxases (MobL)

(11-438)


  Protein structure


Source ID Structure
AlphaFold DB B5U8V6


T4CP

ID   4241 GenBank   WP_002311855
Name   t4cp2_EFTG_RS20585_1,231,410|unnamed insolico UniProt ID   _
Length   952 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 952 a.a.        Molecular weight: 108225.12 Da        Isoelectric Point: 7.4751

>WP_002311855.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Enterococcus]
MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIEGEDLPKSLQDKLNVPLVFIGVFLGSI
VFLAITYLLNFLKALLVAIGQSESGLLGVEFDPKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNH
ESEKNLVYGQKGDSRLSTIKEIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGK
GETFVVPLLDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVIDSWA
NKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDCIEKITMYNVAQMLNEL
GSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGDKAKGSILSTASQGIEMFTSDLFGKLTSK
MSIDLKEIGFPKSIQFKVNTNLVGKRISISFLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLD
IRYEEEKSRAWYRIEFPKKQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNH
VLASIFISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVIQSYSQL
ETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVE
RASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEIDIACEHINLSL
QDNQIPYARFIKDLKTRLEYSIINNIPISEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLS
EQEMKINIYLKESQKEAIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQ
IMILEQQLANAYELAVFKTKNSILKNKIIKLKNAMTKLAKAI

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(199-442)

TraM recognition site of TraD and TraG (TraG-D_C)

(589-701)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 20848..48348

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EFTG_RS20530 (EFTG_05419) 16129..19758 + 3630 WP_002311844 SpaA isopeptide-forming pilin-related protein -
EFTG_RS20535 (EFTG_05420) 19818..20180 + 363 WP_002311845 hypothetical protein -
EFTG_RS22270 (EFTG_05421) 20194..20346 + 153 WP_002311846 hypothetical protein -
EFTG_RS20540 (EFTG_05422) 20414..20836 + 423 WP_002311847 hypothetical protein -
EFTG_RS20545 (EFTG_05423) 20848..22263 + 1416 WP_002311848 CpaF/VirB11 family protein virB11
EFTG_RS20550 (EFTG_05424) 22280..23095 + 816 WP_002313315 hypothetical protein -
EFTG_RS20555 (EFTG_05425) 23095..23892 + 798 WP_002311850 hypothetical protein -
EFTG_RS20560 (EFTG_05426) 23910..24272 + 363 WP_002311851 DUF4320 family protein -
EFTG_RS22515 24536..24664 + 129 WP_002317412 hypothetical protein -
EFTG_RS20570 (EFTG_05427) 24683..24958 + 276 WP_002311852 TrbC/VirB2 family protein virB2
EFTG_RS20575 (EFTG_05428) 25313..26893 + 1581 WP_224997648 hypothetical protein -
EFTG_RS20580 (EFTG_05429) 26906..27622 + 717 WP_002311854 hypothetical protein -
EFTG_RS20585 (EFTG_05430) 27635..30493 + 2859 WP_002311855 VirD4-like conjugal transfer protein, CD1115 family -
EFTG_RS20590 (EFTG_05431) 30544..33180 + 2637 WP_002313318 pLS20_p028 family conjugation system transmembrane protein -
EFTG_RS20595 (EFTG_05432) 33228..33539 + 312 WP_002317413 DUF5592 family protein -
EFTG_RS20600 (EFTG_05433) 33536..34153 + 618 WP_002311858 hypothetical protein virb4
EFTG_RS20605 (EFTG_05434) 34169..34636 + 468 WP_002311859 hypothetical protein -
EFTG_RS20610 (EFTG_05435) 34652..36607 + 1956 WP_002311860 tra protein virb4
EFTG_RS20615 (EFTG_05436) 36629..37765 + 1137 WP_002311861 bifunctional lytic transglycosylase/C40 family peptidase orf14
EFTG_RS20620 (EFTG_05437) 37780..38430 + 651 WP_002311862 cell division protein FtsL -
EFTG_RS20625 (EFTG_05438) 38444..39340 + 897 WP_002311863 hypothetical protein -
EFTG_RS20630 (EFTG_05439) 39376..39606 + 231 WP_002311864 hypothetical protein -
EFTG_RS20635 (EFTG_05440) 39957..40253 + 297 WP_002311865 hypothetical protein -
EFTG_RS20640 (EFTG_05441) 40250..40717 + 468 WP_002311867 hypothetical protein -
EFTG_RS20645 (EFTG_05442) 40799..42319 + 1521 WP_002326870 MobP2 family relaxase -
EFTG_RS20650 (EFTG_05443) 42341..42670 + 330 WP_002311869 hypothetical protein -
EFTG_RS20655 (EFTG_05444) 42672..42935 + 264 WP_002311870 hypothetical protein -
EFTG_RS20660 (EFTG_05445) 43110..43976 + 867 WP_002311871 hypothetical protein -
EFTG_RS20665 (EFTG_05446) 44131..45957 + 1827 WP_002311872 ArdC-like ssDNA-binding domain-containing protein -
EFTG_RS20670 (EFTG_05447) 45965..47038 + 1074 WP_002311873 hypothetical protein -
EFTG_RS20675 (EFTG_05448) 47047..47307 + 261 WP_002311874 hypothetical protein -
EFTG_RS20680 (EFTG_05449) 47344..48348 + 1005 WP_002311875 DUF3991 domain-containing protein traP
EFTG_RS20685 (EFTG_05450) 48373..48729 + 357 WP_002311876 hypothetical protein -
EFTG_RS20690 (EFTG_05451) 48891..49082 + 192 WP_002311877 hypothetical protein -
EFTG_RS20695 (EFTG_05452) 49191..49409 + 219 WP_002311878 hypothetical protein -
EFTG_RS20700 (EFTG_05453) 49446..49763 + 318 WP_002311879 hypothetical protein -
EFTG_RS20705 (EFTG_05454) 49774..49971 + 198 WP_002311880 hypothetical protein -
EFTG_RS20710 (EFTG_05455) 49968..50936 + 969 WP_002311881 zeta toxin family protein -
EFTG_RS20715 (EFTG_05456) 51180..52352 + 1173 WP_002311882 hypothetical protein -
EFTG_RS20720 (EFTG_05457) 52912..53289 - 378 WP_002305129 type II toxin-antitoxin system HicB family antitoxin -

Host bacterium

ID   6746 GenBank   NZ_GG692535
Plasmid name   1,231,410|unnamed Incompatibility group   -
Plasmid size   60477 bp Coordinate of oriT [Strand]   39677..39868 [-]
Host baterium   Enterococcus faecium 1231410

Cargo genes

Drug resistance gene   dfrG
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA1