Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106306
Name   oriT_EbN1|2 in_silico
Organism   Aromatoleum aromaticum EbN1
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NC_006824 (58472..58570 [+], 99 nt)
oriT length   99 nt
IRs (inverted repeats)     _
Location of nic site      61..62
Conserved sequence flanking the
  nic site    		  TCCTGCCCGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 99 nt

>oriT_EbN1|2
GAATAAGGGTTAGCGAAGGTAGATAACCGGCTCCGCTGGTTAGCTAACTTCGCCCATCCTGCCCGCCTTTCGGCGTTCATTACCCCAGGGAAAGTCTAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4217 GenBank   WP_011255046
Name   Relaxase_EBN1_RS22090_EbN1|2 insolico UniProt ID   Q5NWI9
Length   740 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 740 a.a.        Molecular weight: 81425.10 Da        Isoelectric Point: 10.7479

>WP_011255046.1 TraI/MobA(P) family conjugative relaxase [Aromatoleum aromaticum]
MIAKHVPMRSLAKSDFAELVEYITDEQGKTERLGPVTATHCAAATLQAVIGEVLATQRQNTRGAGDKTYH
LLVSFRPGEHPSADTLKVIEERICAGLGYGEHQRVSAVHHDTDNLHLHIAINKIHPERHTMHEPFQSYRT
LAELCAVLERDCGLERDNHQSRRSVAEGRAADMERHAGVESLVGWVRRESLEEMRGAQSWTELHQVMRKN
GLELRERGNGLVVEAADGTRVKASTIARELSKPGLEARLGPFEPSPDRLAEPKMRRQYEKPPIGLRVNTV
ELYARYKAEQQTLTAARADSLAKARRRKDRAVDGAKRANRLRRATITVVDSKGVGKKALYSQASTALRDQ
LAEIHKEYAKEREKLSQGFKRRAWADWLKQEALQGNADALAALRGREAAQGLKGNTIAGQGATKSERAHV
QDNITKKGTIIFRIGQSAVRDDGDKLQVSRDATREGVQAALRLAMERYGSQITVNGTPAFKAQIIRAAVD
SQLPITFADPALELRRQQLLSKEKAHERAARTERGRTDRRRLGGAGSSAAADDNRARTGAGAGRGGSDGR
GGAGMDAPAHVGRKPNVGRIGRVPPPQSQHRLRGMSELSVVRIVGGGEVLLPRDVPRHVEQQRAQSDHAL
RRDAVGVGRLKPAQQAAADEYLAEREDKPLKYFDIPKNSLYNKNARGFSFAGLRHVEGRTLALLRRDDAD
AVMVLPIDQATARRLSRVAVGDPVTVTPRGSIQTSKGRSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(13-251)

Large polyvalent protein-associated domain 7 (LPD7)

(420-509)


  Protein structure


Source ID Structure
AlphaFold DB Q5NWI9


T4CP

ID   4238 GenBank   WP_011255080
Name   t4cp2_EBN1_RS21890_EbN1|2 insolico UniProt ID   _
Length   845 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 845 a.a.        Molecular weight: 93764.76 Da        Isoelectric Point: 6.2164

>WP_011255080.1 VirB4 family type IV secretion/conjugal transfer ATPase [Aromatoleum aromaticum]
MIETIAITIAGVGTVLLLILFVRIRQVDAELQLKKHRSKDAGLADLLNYAAVVDDGVIVGKNGSFMAAWL
YRGDDNASSTDEQREMVSFRLNQALAGLGSGWMVHVDAVRRPAPNYSAKGSSHFPDAVSAAIDKERRQLF
EGLGTMYEGYFVLTLTWFPPLLAQRKFVELMFDDDAIAPDRQTRTMGLIEQFKRELTAIENRLSSAVKLM
RMRGQKVVNEDGSTVTHDDFLSWLQFCLTGRHHPILLPSNPVYLDAVIGGQELWGGVVPRIGRNFVQVVA
IEGFPLESTPGLLTALGELPCEYRWSSRFIFMDPHEAVKHLDKFRKKWRQKVRGFFDQVFNTHTGPVDQD
ALSMVGDAEAAIAEVNSGLVAVGYFTGVVILMDENRDKLEESARLVEKSINRLGFAARIETINTLDAFLG
SLPGHGVENVRRPLINTMNLADLLPTSSIWTGHADAPCPLYPPLSPALMHCVTHGATPFRLNLHVRDIGH
TFMFGPTGAGKSTHLALIAAQLRRYAGMTLYAFDKGMSLYPLTKAVGGLHFSVAADDDRLAFCPLQFLDT
KGDRAWAMEWIDTILALNGVETTPAQRNEIGHAIMSMHRSGGRTLSELSVTIQDEAIREAIRQYTVDGTM
GHLLDAEEDGLSLADFTTFEIEELMDLGEKYALPVLLYLFRRIERSLKGQPAVIILDEAWLMLGHPAFRA
KIREWLKVLRKANCLVLMATQSLSDVANSGILDVIVESTATKIFLPNVYACDQDTAALYRRMGLNARQIE
ILATAIPKRQYYYVSESGRRLYDLALGPFALAFVGSSDKESVASIKSLEAKFGDQWVHEWLAGKGLNLND
YLEAA

  Protein domains


Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(221-431)

TraG P-loop domain (P-loop_TraG)

(619-747)

  Protein structure



No available structure.



ID   4239 GenBank   WP_011255047
Name   t4cp2_EBN1_RS22085_EbN1|2 insolico UniProt ID   _
Length   634 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 634 a.a.        Molecular weight: 69736.77 Da        Isoelectric Point: 7.6457

>WP_011255047.1 type IV secretory system conjugative DNA transfer family protein [Aromatoleum aromaticum]
MKIKMDNAVGPQVRTKKGAGGKLLPVLGAVSLGAGLQSATQFFAHIFDYQANLGGHFNHVYAPWSILEWS
SKWYSHYPNEIMQAGSVGMMISTVGLLGVAVAKVVSSNSSKANEYLHGSARWAGKKDIQAAGLLPRPRRL
LDVVTGKDTAPSTGVYVGGWEDKDGNFHYLRHNGPEHILTYAPTRSGKGVGLVVPTLLSWGDSAVVTDLK
GELWAMTAGWRKKHAKNNVLRFEPATANGSVCWNPLDEIRMGTEHEVGDVQNLVTLIVDPDGKGLGSHWQ
KTAFALLVGVILHALYKAKDDDTTATLPSVDAMLADPNRDVGELWMEMATYGHVAGQNHHAIGSAARDMM
DRPEEEAGSVLSTAKSFLALYRDPVVARNVSRSEFCIRDLMHSDDPVTLYIVTHPNDKDRLRPLVRVMVN
MIVRLLADKMDFDNGRLVAHYKHRLLMMLDEFPSLGKLQILQESLAFVAGYGIKCYLICQDINQLKSRET
GYGPDETITSNCHVQNAYPPNRVETAEHLSRLTGQTTVVKEQITTSGRRTAAMLGQVSRTFQEVQRPLLT
PDECLRMPGPRKNADGNIEEPGDMVVYVAGYPAIYGKQPLYFKDPIFLARAAVPAPKDSDRLVQFAEAGE
RITI

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(121-615)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 12707..23216

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EBN1_RS21835 (EB_RS21435) 8539..9594 + 1056 WP_011255087 2-oxoacid:ferredoxin oxidoreductase subunit beta -
EBN1_RS21840 (EB_RS21440) 9664..10023 + 360 Protein_7 electron transfer flavoprotein subunit beta/FixA family protein -
EBN1_RS21845 (EB_RS25080) 10025..10211 - 187 Protein_8 electron transfer flavoprotein subunit beta/FixA family protein -
EBN1_RS21850 (EB_RS24560) 10211..10351 + 141 WP_197531916 hypothetical protein -
EBN1_RS21855 (EB_RS21445) 10689..10910 + 222 WP_041647956 hypothetical protein -
EBN1_RS21860 (EB_RS24565) 11115..11493 + 379 Protein_11 IS21 family transposase -
EBN1_RS21865 11537..11779 - 243 Protein_12 plasmid replication initiator TrfA -
EBN1_RS21870 (EB_RS21455) 12049..12411 + 363 WP_011255084 transcriptional regulator -
EBN1_RS21875 (EB_RS21460) 12707..13666 + 960 WP_011255083 P-type conjugative transfer ATPase TrbB virB11
EBN1_RS21880 (EB_RS21465) 13680..14105 + 426 WP_011255082 conjugal transfer system pilin TrbC virB2
EBN1_RS21885 (EB_RS21470) 14108..14419 + 312 WP_011255081 conjugal transfer protein TrbD virB3
EBN1_RS21890 (EB_RS21475) 14416..16953 + 2538 WP_011255080 VirB4 family type IV secretion/conjugal transfer ATPase virb4
EBN1_RS21895 (EB_RS21480) 16950..17735 + 786 WP_011255079 conjugal transfer protein TrbF virB8
EBN1_RS21900 (EB_RS21485) 17753..18649 + 897 WP_011255078 P-type conjugative transfer protein TrbG virB9
EBN1_RS21905 (EB_RS21490) 18652..19143 + 492 WP_041647958 conjugal transfer protein TrbH -
EBN1_RS21910 (EB_RS21495) 19149..20537 + 1389 WP_011255076 TrbI/VirB10 family protein virB10
EBN1_RS21915 (EB_RS21500) 20556..21314 + 759 WP_011255075 P-type conjugative transfer protein TrbJ virB5
EBN1_RS21920 (EB_RS21505) 21329..21559 + 231 WP_011255074 entry exclusion lipoprotein TrbK -
EBN1_RS21925 (EB_RS21510) 21570..23216 + 1647 WP_011255073 P-type conjugative transfer protein TrbL virB6
EBN1_RS21930 (EB_RS21515) 23234..23812 + 579 WP_011255072 TrbM/KikA/MpfK family conjugal transfer protein -
EBN1_RS21935 (EB_RS21520) 23818..24414 + 597 WP_041648023 muramidase -
EBN1_RS21940 (EB_RS21525) 24411..25109 + 699 WP_011255070 TraX family protein -
EBN1_RS21945 (EB_RS21530) 25204..25629 + 426 WP_041647959 hypothetical protein -
EBN1_RS21950 (EB_RS21535) 25716..26015 + 300 WP_041647960 DUF4258 domain-containing protein -
EBN1_RS21955 (EB_RS21540) 26017..27840 + 1824 WP_157866790 N-6 DNA methylase -

Host bacterium

ID   6743 GenBank   NC_006824
Plasmid name   EbN1|2 Incompatibility group   -
Plasmid size   223670 bp Coordinate of oriT [Strand]   58472..58570 [+]
Host baterium   Aromatoleum aromaticum EbN1

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -