Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 106304
• Name: oriT_DS5|unnamed
• Organism: Enterococcus faecalis DS5
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_GG692896 (22414..22463 [+], 50 nt)
• oriT length: 50 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 50 nt

>oriT_DS5|unnamed
AATATCGCAACATGCTAGCATGTTGCTCTGCTCGCAAAAAGAAAGCCTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_EFEG_RS21310_DS5|unnamed

• ID: 4215
• GenBank: WP_002365873
• Name: Relaxase_EFEG_RS21310_DS5|unnamed
• UniProt ID: _
• Length: 561 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 561 a.a.
• Molecular weight: 65469.22 Da
• Isoelectric Point: 6.0174

>WP_002365873.1 relaxase/mobilization nuclease domain-containing protein [Enterococcus faecalis]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKRVFEQISDKIAAKEGAKIIEKSPKNS
HKKYTMWETESLYKEKIKSRLDFLLEQSSSINDFLEKAEALNLSVDFSKKWTTYRLLDEPQIKNTRSRSL
SKNDPTRYNYEKIVERLKENKSVLTLKEVVEKYVEKSEKTKNEFDYQLTIDEWQISHKTTRGYYVNVDFG
FGERGKVFIGAYKVDPLENGQYNIYVKRKDFFYLMNEKNADRNRYMTGETLIKQLRLYNGQTPLKKEPVM
RTIDELVNAINFLAANEIEDTRQLELLEEKLEAAFLEAEETLETLDEKMLELHQLSNLLLENELNGELEL
IQQKLKTLLPDATLAEFSYEDVRGEIEAIKTSQSLLENKLERTRNEINQLHEIQAVQKKETANQNNVKPK
L

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (37-296)

Protein structure

No available structure.

Auxiliary protein

WP_002365872_DS5|unnamed

• ID: 1879
• GenBank: WP_002365872
• Name: WP_002365872_DS5|unnamed
• UniProt ID: _
• Length: 118 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

Auxiliary protein sequence

• Length: 118 a.a.
• Molecular weight: 13988.05 Da
• Isoelectric Point: 9.6548

>WP_002365872.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Enterococcus]
MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVHSLNALVQSELNKLIKRKEQS

Protein domains

Predicted by InterproScan.

• Bacterial mobilisation protein (MobC) (MobC) (64-105)

Protein structure

No available structure.

T4CP

t4cp2_EFEG_RS21290_DS5|unnamed

• ID: 4237
• GenBank: WP_002365870
• Name: t4cp2_EFEG_RS21290_DS5|unnamed
• UniProt ID: _
• Length: 609 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 609 a.a.
• Molecular weight: 70184.31 Da
• Isoelectric Point: 6.5169

>WP_002365870.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNVAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEVKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRALEERKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (144-558)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 3466..19769

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFEG_RS21210 (EFEG_05301)	1..1412	+	1412	Protein_0	surface exclusion protein SEA1/PrgA	-
EFEG_RS21215 (EFEG_05302)	3466..5805	+	2340	WP_002365851	LPXTG-anchored aggregation substance	prgB
EFEG_RS21220 (EFEG_05304)	6442..6798	+	357	WP_002365854	pheromone response system RNA-binding regulator PrgU	-
EFEG_RS21225 (EFEG_05305)	6826..7701	+	876	WP_002365855	pheromone response LPXTG-anchored adhesin PrgC	prgC
EFEG_RS21230 (EFEG_05306)	7743..8642	+	900	WP_025186515	hypothetical protein	-
EFEG_RS21235 (EFEG_05307)	8662..9096	+	435	WP_002365857	hypothetical protein	-
EFEG_RS21240 (EFEG_05308)	9143..9370	+	228	WP_002365858	hypothetical protein	-
EFEG_RS21245 (EFEG_05309)	9384..9680	+	297	WP_002365860	hypothetical protein	-
EFEG_RS21250 (EFEG_05310)	9695..10495	+	801	WP_002365861	PrgH	prgHb
EFEG_RS21255 (EFEG_05311)	10497..10850	+	354	WP_002365862	PrgI family mobile element protein	prgIc
EFEG_RS21260 (EFEG_05312)	10804..13194	+	2391	WP_002365863	VirB4-like conjugal transfer ATPase, CD1110 family	virb4
EFEG_RS21265 (EFEG_05313)	13206..15821	+	2616	WP_002365864	phage tail tip lysozyme	prgK
EFEG_RS21270 (EFEG_05314)	15845..16471	+	627	WP_002365865	hypothetical protein	prgL
EFEG_RS21275 (EFEG_05315)	16458..16703	+	246	WP_002365866	hypothetical protein	-
EFEG_RS21280 (EFEG_05316)	16687..17295	+	609	WP_002365867	hypothetical protein	-
EFEG_RS21285 (EFEG_05317)	17455..17940	+	486	WP_016616031	PcfB family protein	gbs1365
EFEG_RS21290 (EFEG_05318)	17940..19769	+	1830	WP_002365870	VirD4-like conjugal transfer protein, CD1115 family	virb4
EFEG_RS21295 (EFEG_05319)	19820..21979	+	2160	WP_002365871	ArdC-like ssDNA-binding domain-containing protein	-
EFEG_RS21300 (EFEG_05320)	22012..22284	+	273	WP_002362409	LtrD	-
EFEG_RS21305 (EFEG_05321)	22530..22886	+	357	WP_002365872	plasmid mobilization relaxosome protein MobC	-
EFEG_RS21310 (EFEG_05322)	22887..24572	+	1686	WP_002365873	relaxase/mobilization nuclease domain-containing protein	-

Host bacterium

• ID: 6741
• GenBank: NZ_GG692896
• Plasmid name: DS5|unnamed
• Incompatibility group: -
• Plasmid size: 55755 bp
• Coordinate of oriT [Strand]: 22414..22463 [+]
• Host baterium: Enterococcus faecalis DS5

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -