Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 106302 |
| Name | oriT_AR01/DG|unnamed  |
| Organism | Enterococcus faecalis ARO1/DG strain AR01/DG |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_GG692907 (28772..28821 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_AR01/DG|unnamed
AATATCGCAACATGGTACCATGTTGCTCTGCTCGCAAAAAGAAAGCCTAC
AATATCGCAACATGGTACCATGTTGCTCTGCTCGCAAAAAGAAAGCCTAC
Visualization of oriT structure
Relaxase
| ID | 4213 | GenBank | WP_002367697 |
| Name | Relaxase_EFFG_RS19150_AR01/DG|unnamed |
UniProt ID | _ |
| Length | 561 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 561 a.a. Molecular weight: 65315.04 Da Isoelectric Point: 6.1225
>WP_002367697.1 relaxase/mobilization nuclease domain-containing protein [Enterococcus faecalis]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKKVFEQISDKIAAKEGAKIIEKSPKNS
HKKYTMWETESLYKEKIKSRLDFLLEQSSSINDFLEKAEALNLSVDFSKKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIVERLKENKSVLTLKEVVEKYGEKSEKSKNEFDYQLTIDEWQISHKTTRGYYVNVDFG
FGERGKVFIGAYKVDPLENGQYNIYVKRKDFFYLMNEKNADRNRYMTGETLIKQLRLYNGQTPLKKEPVM
QTIDELVNAINFLAANEIDDTRQLELLEEKLEAAFLEAEETLETLDEKMLELHQLSNLLLENELNGELEL
IQQKLKTLLPDATLAEFSYKDVRGEIEAIKTSQSLLENKLERTRNEINQLHEIQAVQKKETANQNNVKPK
L
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKKVFEQISDKIAAKEGAKIIEKSPKNS
HKKYTMWETESLYKEKIKSRLDFLLEQSSSINDFLEKAEALNLSVDFSKKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIVERLKENKSVLTLKEVVEKYGEKSEKSKNEFDYQLTIDEWQISHKTTRGYYVNVDFG
FGERGKVFIGAYKVDPLENGQYNIYVKRKDFFYLMNEKNADRNRYMTGETLIKQLRLYNGQTPLKKEPVM
QTIDELVNAINFLAANEIDDTRQLELLEEKLEAAFLEAEETLETLDEKMLELHQLSNLLLENELNGELEL
IQQKLKTLLPDATLAEFSYKDVRGEIEAIKTSQSLLENKLERTRNEINQLHEIQAVQKKETANQNNVKPK
L
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 1878 | GenBank | WP_002367696 |
| Name | WP_002367696_AR01/DG|unnamed |
UniProt ID | Q82YL4 |
| Length | 118 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 118 a.a. Molecular weight: 14042.10 Da Isoelectric Point: 9.9724
>WP_002367696.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Bacteria]
MENKQKLKRPIQRKIRFSKEENELINRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKEQS
MENKQKLKRPIQRKIRFSKEENELINRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKEQS
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q82YL4 |
T4CP
| ID | 4236 | GenBank | WP_002367691 |
| Name | t4cp2_EFFG_RS19130_AR01/DG|unnamed |
UniProt ID | _ |
| Length | 609 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 609 a.a. Molecular weight: 70145.14 Da Isoelectric Point: 6.1807
>WP_002367691.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRALEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRALEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 7390..26127
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EFFG_RS19020 (EFFG_05003) | 2573..2953 | + | 381 | WP_002367664 | hypothetical protein | - |
| EFFG_RS19025 (EFFG_05005) | 3524..3829 | + | 306 | WP_002367666 | hypothetical protein | - |
| EFFG_RS19030 (EFFG_05006) | 3840..6512 | + | 2673 | WP_002367667 | surface exclusion protein SEA1/PrgA | - |
| EFFG_RS19035 (EFFG_05007) | 6578..7165 | + | 588 | WP_002367669 | hypothetical protein | - |
| EFFG_RS19040 (EFFG_05008) | 7390..11304 | + | 3915 | WP_002367670 | LPXTG-anchored aggregation substance PrgB/Asc10 | prgB |
| EFFG_RS19045 (EFFG_05009) | 11366..11722 | + | 357 | WP_002367672 | pheromone response system RNA-binding regulator PrgU | - |
| EFFG_RS19050 (EFFG_05010) | 11750..12607 | + | 858 | WP_002367673 | pheromone response LPXTG-anchored adhesin PrgC | prgC |
| EFFG_RS19055 (EFFG_05011) | 12648..13547 | + | 900 | WP_025186739 | hypothetical protein | - |
| EFFG_RS19060 (EFFG_05012) | 13567..14001 | + | 435 | WP_002365857 | hypothetical protein | - |
| EFFG_RS19065 (EFFG_05013) | 14048..14275 | + | 228 | WP_002365858 | hypothetical protein | - |
| EFFG_RS19070 (EFFG_05014) | 14288..14584 | + | 297 | WP_002367677 | hypothetical protein | - |
| EFFG_RS19075 (EFFG_05015) | 14599..15399 | + | 801 | WP_002367679 | type IV secretion system protein | prgHb |
| EFFG_RS19080 (EFFG_05016) | 15401..15754 | + | 354 | WP_002365862 | PrgI family mobile element protein | prgIc |
| EFFG_RS19085 (EFFG_05017) | 15708..18098 | + | 2391 | WP_002365863 | VirB4-like conjugal transfer ATPase, CD1110 family | virb4 |
| EFFG_RS19090 (EFFG_05018) | 18110..20725 | + | 2616 | WP_002367681 | phage tail tip lysozyme | prgK |
| EFFG_RS19095 (EFFG_05019) | 20749..21375 | + | 627 | WP_002367683 | hypothetical protein | prgL |
| EFFG_RS19100 (EFFG_05020) | 21362..21607 | + | 246 | WP_002365866 | hypothetical protein | - |
| EFFG_RS19105 (EFFG_05021) | 21591..22199 | + | 609 | WP_002367686 | hypothetical protein | - |
| EFFG_RS19110 (EFFG_05022) | 22313..23068 | + | 756 | WP_002367687 | Fic family protein | - |
| EFFG_RS19115 (EFFG_05023) | 23085..23543 | + | 459 | WP_002367688 | single-stranded DNA-binding protein | - |
| EFFG_RS19120 (EFFG_05024) | 23589..23771 | + | 183 | WP_002367689 | hypothetical protein | - |
| EFFG_RS19125 (EFFG_05025) | 23813..24298 | + | 486 | WP_002386493 | PcfB family protein | gbs1365 |
| EFFG_RS19130 (EFFG_05026) | 24298..26127 | + | 1830 | WP_002367691 | VirD4-like conjugal transfer protein, CD1115 family | virb4 |
| EFFG_RS19135 (EFFG_05027) | 26178..28337 | + | 2160 | WP_002367693 | ArdC-like ssDNA-binding domain-containing protein | - |
| EFFG_RS19140 (EFFG_05028) | 28370..28642 | + | 273 | WP_002367695 | LtrD | - |
| EFFG_RS19145 (EFFG_05029) | 28888..29244 | + | 357 | WP_002367696 | plasmid mobilization relaxosome protein MobC | - |
| EFFG_RS19150 (EFFG_05030) | 29245..30930 | + | 1686 | WP_002367697 | relaxase/mobilization nuclease domain-containing protein | - |
Host bacterium
| ID | 6739 | GenBank | NZ_GG692907 |
| Plasmid name | AR01/DG|unnamed | Incompatibility group | - |
| Plasmid size | 55443 bp | Coordinate of oriT [Strand] | 28772..28821 [+] |
| Host baterium | Enterococcus faecalis ARO1/DG strain AR01/DG |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | prgB/asc10 |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA9 |