Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 106289 |
| Name | oriT_163013|unnamed1  |
| Organism | Salmonella enterica subsp. enterica serovar Blockley strain 163013 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CM019093 (6999..7347 [-], 349 nt) |
| oriT length | 349 nt |
| IRs (inverted repeats) | 134..140, 154..160 (AAAATAT..ATATTTT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 349 nt
>oriT_163013|unnamed1
ACTGCGATGCTGGATAGGGCAAACGCCGCAAAATGACGTCTCTGACGCCATTCCGCAACGTTTGCCCGATGACCTACAGTCGGTTCTTGTTGGAGAGTTTTATGAAATATTTGGTCAAAGAGTTTATTAACGAAAAATATACTAAGGCTGTTAATATTTTAAAGGATAACCTTAAAGAACACTATCATGTTTTTTATTGTGTGAGATTAAGTGAGATTCTTTTTCCTGCCAGTGAATATGGAACTGAACAGTTTTTTAGTGATTTTGAAAAGATAAACTCCATTTCATTACCTGTCTTAGTGTTTGATCTCAAAGAGGGTGTTCCTGTGATTGTCATTAGTCTTGATGA
ACTGCGATGCTGGATAGGGCAAACGCCGCAAAATGACGTCTCTGACGCCATTCCGCAACGTTTGCCCGATGACCTACAGTCGGTTCTTGTTGGAGAGTTTTATGAAATATTTGGTCAAAGAGTTTATTAACGAAAAATATACTAAGGCTGTTAATATTTTAAAGGATAACCTTAAAGAACACTATCATGTTTTTTATTGTGTGAGATTAAGTGAGATTCTTTTTCCTGCCAGTGAATATGGAACTGAACAGTTTTTTAGTGATTTTGAAAAGATAAACTCCATTTCATTACCTGTCTTAGTGTTTGATCTCAAAGAGGGTGTTCCTGTGATTGTCATTAGTCTTGATGA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 4208 | GenBank | WP_000539540 |
| Name | mobP1_F1E30_RS00125_163013|unnamed1 |
UniProt ID | A0A744DX50 |
| Length | 388 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 388 a.a. Molecular weight: 44505.86 Da Isoelectric Point: 10.5067
>WP_000539540.1 MULTISPECIES: MobP1 family relaxase [Enterobacteriaceae]
MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAVTRQGIRNSI
DYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENKKITQNIVFSPPVSAKVKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GDLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFWGL
MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAVTRQGIRNSI
DYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENKKITQNIVFSPPVSAKVKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GDLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFWGL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A744DX50 |
T4CP
| ID | 4230 | GenBank | WP_080898538 |
| Name | t4cp2_F1E30_RS00190_163013|unnamed1 |
UniProt ID | _ |
| Length | 611 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 611 a.a. Molecular weight: 69359.20 Da Isoelectric Point: 7.1491
>WP_080898538.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESNIRTDIRLTAIPS
LLSGMVSSLIVPVFIIWQLNKTDVALYGDAKFASDNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKSRGRSTSQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPY
FMDRLMKVSPKLASLAMELNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL
MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESNIRTDIRLTAIPS
LLSGMVSSLIVPVFIIWQLNKTDVALYGDAKFASDNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKSRGRSTSQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPY
FMDRLMKVSPKLASLAMELNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 16499..26460
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| F1E30_RS00090 (F1E30_23125) | 11815..12060 | + | 246 | WP_000356542 | hypothetical protein | - |
| F1E30_RS00095 (F1E30_23130) | 12050..12265 | + | 216 | WP_001180117 | hypothetical protein | - |
| F1E30_RS00100 (F1E30_23135) | 12358..12687 | + | 330 | WP_000866654 | hypothetical protein | - |
| F1E30_RS00105 (F1E30_23140) | 12730..12909 | + | 180 | WP_021526659 | hypothetical protein | - |
| F1E30_RS23725 | 12931..13128 | + | 198 | WP_077688582 | hypothetical protein | - |
| F1E30_RS00115 (F1E30_23145) | 13141..13413 | + | 273 | WP_000160399 | hypothetical protein | - |
| F1E30_RS00120 (F1E30_23150) | 13739..14284 | + | 546 | WP_000757693 | DNA distortion polypeptide 1 | - |
| F1E30_RS00125 (F1E30_23155) | 14287..15453 | + | 1167 | WP_000539540 | MobP1 family relaxase | - |
| F1E30_RS00130 (F1E30_23160) | 15806..16324 | + | 519 | WP_001446885 | transcription termination/antitermination NusG family protein | - |
| F1E30_RS00135 (F1E30_23165) | 16254..16466 | + | 213 | WP_000125172 | hypothetical protein | - |
| F1E30_RS00140 (F1E30_23170) | 16499..17146 | + | 648 | WP_000953529 | lytic transglycosylase domain-containing protein | virB1 |
| F1E30_RS00145 (F1E30_23175) | 17124..17420 | + | 297 | WP_000916182 | TrbC/VirB2 family protein | virB2 |
| F1E30_RS00150 (F1E30_23180) | 17439..20192 | + | 2754 | WP_065327995 | VirB3 family type IV secretion system protein | virb4 |
| F1E30_RS00155 (F1E30_23185) | 20203..20961 | + | 759 | WP_000744210 | type IV secretion system protein | - |
| F1E30_RS00160 (F1E30_23190) | 20962..21189 | + | 228 | WP_000835348 | EexN family lipoprotein | - |
| F1E30_RS00165 (F1E30_23195) | 21199..22332 | + | 1134 | WP_000513458 | type IV secretion system protein | virB6 |
| F1E30_RS00170 (F1E30_23200) | 22578..23291 | + | 714 | WP_000394612 | type IV secretion system protein | virB8 |
| F1E30_RS00175 (F1E30_23205) | 23296..24225 | + | 930 | WP_000783385 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| F1E30_RS00180 (F1E30_23210) | 24222..25427 | + | 1206 | WP_031622284 | VirB10/TraB/TrbI family type IV secretion system protein | virB10 |
| F1E30_RS00185 (F1E30_23215) | 25429..26460 | + | 1032 | WP_001058468 | P-type DNA transfer ATPase VirB11 | virB11 |
| F1E30_RS00190 (F1E30_23220) | 26463..28298 | + | 1836 | WP_080898538 | type IV secretory system conjugative DNA transfer family protein | - |
| F1E30_RS00195 (F1E30_23225) | 28295..28708 | + | 414 | WP_080898537 | cag pathogenicity island Cag12 family protein | - |
| F1E30_RS00200 (F1E30_23230) | 28705..29007 | + | 303 | WP_000717627 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| F1E30_RS23735 | 29120..29314 | + | 195 | Protein_40 | ATP-binding protein | - |
| F1E30_RS00205 (F1E30_23235) | 29318..29893 | + | 576 | WP_063320189 | hypothetical protein | - |
Host bacterium
| ID | 6726 | GenBank | NZ_CM019093 |
| Plasmid name | 163013|unnamed1 | Incompatibility group | IncX1 |
| Plasmid size | 36629 bp | Coordinate of oriT [Strand] | 6999..7347 [-] |
| Host baterium | Salmonella enterica subsp. enterica serovar Blockley strain 163013 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |