Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   106257
Name   oriT_pRWS291.s5 in_silico
Organism   Klebsiella pneumoniae strain WS_29-1
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP110519 (13646..15352 [+], 1707 nt)
oriT length   1707 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 1707 nt

>oriT_pRWS291.s5
GCCGTATGAATGACATCTATTTCCGTCCAATTCATCGAAACCAGTATTTTGAGGAAATGAAAGCATGATAAAGCAAATTAAAGTAATCATGTTGGCAGCAATAGTTACCGGATGTTCATCGCCGCCGCCTCCCGTTCCTGTGGAGTGGGATAAAGCCGCTGACCCATTAAACACCAGTTTACCGCAATGGAGTGACAATAACGTTATAGTCCCTTCCCCGACAGTTAACGGTAAATGGACATCATCAATCAGCGCACAAAGTTTCTACGATACAATATGGACTCCAGCAGTATTTTACGCAGTCGCCCATTCAACACGAATTGTCGTCACTGCACAATCCGGCACTGATTTCTTTAATGCCAAAAACTGGCTTCGCCAGAATGGCGCAAAAGGCGTTATTGAATACCAGCCTGTCTTTAATTGCCTGACGTGCAGAGAAACCACTATTTATTTCTCTCGCTAATCCATTTAAGCAACACAATCTAATAAGGATTTATCATGAAAAAAACCGTTATCGCGGCGGCTGTATTATGCCTGACCGCAGGAACGCCGATTTATTCCTTTGCCGCTGACCCGTGCGAAGTTGTTCTTTGCATGTACGGAAAAGCTACAGGTAATAGTGGTGGGAGTGAATGCAGTTCAGCAGAACGCGCATTTTTTAAAATAAACGCATTTAAAAAACACCATCGTTTCAATCCGGGTAAAACATTCGATATGCGAAAAGAATTTTTAGGGGAGTGCAAAGCCGCAGACCCTGAATCAATAAGCAAAATCCTTTCAAAATTTGGTAGAGTGAAAGGATAACTGACATATCCATTTTTATATGAGATGTTCGGAATGATAAATGAAAATTTATTCATTAAGAATATACACTCAAAAAATCAGGACAGAATATCTGTAGCCCTGGTATATGACACGTTATCAAAAGAAGCCCACAGAGGTTGTGGGCTGTATTACGAAATATACGAAAGTTGTTTTATCGGTCTTTTGCGCGATCATCTTTCAGAACTTAATGAGGTTGACGCAAACAAATTAATACGCTATGCCGAAAGCAAAGGCACAAAAATTGATGATGCGTCCTACAGTGAAGCACTGGAAGCTGAAAGGGAGTGCCGGGCTGAAATATACCGCGAGCAAATGTAATCACACAAAACAAAAGTGTATTGTACAATACACAGACTAACAGGCTACGGGATTTTAAGGCTTCATTATTTTTGGCTTCAATTTCAAAGAAATTGTTAAGACGAAAAATAATGAAGTGGTACGCGGAAAGAAAATTCAATTTCGGAGAAATTGCTAATTTTCAGGAACCGCGCCCACCTGCGAACATTCGCCCACAGCGAATTGAGCAGGCCGGGGGGAGCCGCAAAAATTTTGCCGCCCCAGCGAGCAAAAAGGCACGCCCACGTGCCGTTTTTTGTGGGGGGCTTGCCCCGCACACCGTCGCAGCCAGGTAATATAACCCGCCCCAGCGGGGGATATTACCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCGATTTTTTCGAGCTTGCGAGGAAAAATAGGCTAAACGCAGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCAAGACGGTGTGCCGCCTTTGACCTTGGGGGGTTTTGGCTCGGCGTCCAGCCGGGACATTACCCCCATGTGTCATGCAGTGAGGGCGTCCAGCCCGAACGTAATGGCACACGCCGT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


T4CP

ID   4214 GenBank   WP_012457110
Name   t4cp2_OM421_RS30315_pRWS291.s5 insolico UniProt ID   _
Length   912 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 912 a.a.        Molecular weight: 103056.99 Da        Isoelectric Point: 7.4625

>WP_012457110.1 MULTISPECIES: VirB3 family type IV secretion system protein [Enterobacterales]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGSAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

  Protein domains


Predicted by InterproScan.

Type IV secretory pathway, VirB3-like protein (VirB3)

(3-79)

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(269-470)

TraG P-loop domain (P-loop_TraG)

(535-813)

  Protein structure



No available structure.



ID   4215 GenBank   WP_094309419
Name   t4cp2_OM421_RS30370_pRWS291.s5 insolico UniProt ID   _
Length   578 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 578 a.a.        Molecular weight: 65670.93 Da        Isoelectric Point: 7.4566

>WP_094309419.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLLAGLTLPFIFHLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKSLDNNILFRVPLWIMTIIFTAAGLIFHIAVRRLLAGEIDNLKHRMIKKSKLERNT
RTDVRKVKELLPDSIEYNPLDYIDLKKGVFIGLNKDDQPQYITIKEFKTQHAAIIGTTGSGKGVTATVLL
YQAILLGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKLNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLRDLFNTDFVQSLREDVPAFCGELEEIALVNSINATKGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDSVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKVDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTSTELPRATKMFQVQVKKENIELLSFEYK
QATPEKNESQEHKPSINL

  Protein domains


Predicted by InterproScan.

Helicase HerA, central domain (DUF87)

(174-280)

TraM recognition site of TraD and TraG (TraG-D_C)

(399-490)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 3756..17305

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OM421_RS30275 (OM421_30270) 895..1596 - 702 WP_258549312 RepA family replication protein -
OM421_RS30280 (OM421_30275) 2060..2419 - 360 WP_012457108 helix-turn-helix transcriptional regulator -
OM421_RS30285 (OM421_30280) 2673..3128 + 456 WP_009309974 transcription termination/antitermination NusG family protein -
OM421_RS30290 (OM421_30285) 3200..3415 + 216 WP_004178170 hypothetical protein -
OM421_RS30295 (OM421_30290) 3420..3638 + 219 WP_032722781 hypothetical protein -
OM421_RS30300 (OM421_30295) 3635..3757 - 123 WP_255093128 hypothetical protein -
OM421_RS30305 (OM421_30300) 3756..4466 + 711 WP_004178175 lytic transglycosylase domain-containing protein virB1
OM421_RS30310 (OM421_30305) 4463..4768 + 306 WP_012457109 TrbC/VirB2 family protein virB2
OM421_RS30315 (OM421_30310) 4781..7519 + 2739 WP_012457110 VirB3 family type IV secretion system protein virb4
OM421_RS30320 (OM421_30315) 7538..8248 + 711 WP_012457111 type IV secretion system protein -
OM421_RS30325 (OM421_30320) 8258..8485 + 228 WP_004178109 EexN family lipoprotein -
OM421_RS30330 (OM421_30325) 8497..9573 + 1077 WP_012457112 type IV secretion system protein virB6
OM421_RS30335 (OM421_30330) 9795..10478 + 684 WP_094309417 type IV secretion system protein virB8
OM421_RS30340 (OM421_30335) 10475..11383 + 909 WP_012457114 P-type conjugative transfer protein VirB9 virB9
OM421_RS30345 (OM421_30340) 11430..12698 + 1269 WP_139968916 VirB10/TraB/TrbI family type IV secretion system protein virB10
OM421_RS30350 (OM421_30345) 12688..13713 + 1026 WP_012457116 P-type DNA transfer ATPase VirB11 virB11
OM421_RS30355 (OM421_30350) 13710..14108 + 399 WP_012457117 cag pathogenicity island Cag12 family protein -
OM421_RS30360 (OM421_30355) 14144..14449 + 306 WP_012457118 hypothetical protein -
OM421_RS30365 (OM421_30360) 14483..14788 + 306 WP_094309429 dpoa decarboxylase -
OM421_RS30370 (OM421_30365) 15569..17305 + 1737 WP_094309419 type IV secretion system DNA-binding domain-containing protein virb4
OM421_RS30375 (OM421_30370) 17314..18069 + 756 WP_023316386 MobC family replication-relaxation protein -
OM421_RS30380 (OM421_30375) 18172..18702 + 531 WP_012457122 phospholipase D family protein -
OM421_RS30385 (OM421_30380) 18886..19854 - 969 WP_012457123 zincin-like metallopeptidase domain-containing protein -
OM421_RS30390 (OM421_30385) 20239..20904 + 666 WP_094309421 hypothetical protein -
OM421_RS30395 (OM421_30390) 21067..21216 + 150 WP_162262154 hypothetical protein -
OM421_RS30400 (OM421_30395) 21278..21577 - 300 WP_012457126 helix-turn-helix domain-containing protein -
OM421_RS30405 (OM421_30400) 21580..21930 - 351 WP_012457127 type II toxin-antitoxin system RelE/ParE family toxin -

Host bacterium

ID   6694 GenBank   NZ_CP110519
Plasmid name   pRWS291.s5 Incompatibility group   IncFII
Plasmid size   38030 bp Coordinate of oriT [Strand]   13646..15352 [+]
Host baterium   Klebsiella pneumoniae strain WS_29-1

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -