Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 106243 |
| Name | oriT_pKP102-3  |
| Organism | Klebsiella pneumoniae strain KP102 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP089887 (30091..30195 [-], 105 nt) |
| oriT length | 105 nt |
| IRs (inverted repeats) | 32..37, 40..45 (GCCGGC..GCCGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 105 nt
>oriT_pKP102-3
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 4184 | GenBank | WP_085844212 |
| Name | Relaxase_LR951_RS28485_pKP102-3 |
UniProt ID | _ |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75246.00 Da Isoelectric Point: 9.9948
>WP_085844212.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLIRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQNTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSENDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLIRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQNTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSENDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 4203 | GenBank | WP_004187443 |
| Name | t4cp2_LR951_RS28365_pKP102-3 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79519.87 Da Isoelectric Point: 4.8309
>WP_004187443.1 MULTISPECIES: hypothetical protein [Enterobacterales]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1..27293
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LR951_RS28355 (LR951_28300) | 1..1308 | + | 1308 | WP_015062796 | hypothetical protein | trbA |
| LR951_RS28360 (LR951_28305) | 1319..2269 | + | 951 | WP_277696346 | DsbC family protein | trbB |
| LR951_RS28365 (LR951_28310) | 2282..4369 | + | 2088 | WP_004187443 | hypothetical protein | - |
| LR951_RS28370 | 4420..4515 | - | 96 | WP_004206884 | DinQ-like type I toxin DqlB | - |
| LR951_RS28375 (LR951_28315) | 5741..6796 | - | 1056 | WP_159174226 | plasmid replication initiator RepA | - |
| LR951_RS28380 (LR951_28320) | 7094..7342 | - | 249 | WP_015062836 | IncL/M type plasmid replication protein RepC | - |
| LR951_RS28385 (LR951_28325) | 7399..8052 | - | 654 | WP_004187494 | hypothetical protein | - |
| LR951_RS28390 (LR951_28330) | 8055..10235 | - | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| LR951_RS28395 (LR951_28335) | 10228..10878 | - | 651 | WP_004187488 | hypothetical protein | - |
| LR951_RS28400 (LR951_28340) | 10875..12083 | - | 1209 | WP_004187486 | conjugal transfer protein TraW | traW |
| LR951_RS28405 (LR951_28345) | 12080..15130 | - | 3051 | WP_040119746 | conjugal transfer protein | traU |
| LR951_RS28410 (LR951_28350) | 15127..15621 | - | 495 | WP_011091080 | hypothetical protein | - |
| LR951_RS28415 (LR951_28355) | 15667..16056 | - | 390 | WP_011091078 | DUF6750 family protein | traR |
| LR951_RS28420 (LR951_28360) | 16073..16603 | - | 531 | WP_011091077 | conjugal transfer protein TraQ | traQ |
| LR951_RS28425 (LR951_28365) | 16627..17331 | - | 705 | WP_040119747 | conjugal transfer protein TraP | traP |
| LR951_RS28430 (LR951_28370) | 17343..18692 | - | 1350 | WP_015062830 | conjugal transfer protein TraO | traO |
| LR951_RS28435 (LR951_28375) | 18704..19855 | - | 1152 | WP_011091074 | DotH/IcmK family type IV secretion protein | traN |
| LR951_RS28440 (LR951_28380) | 19864..20577 | - | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| LR951_RS28445 (LR951_28385) | 20645..21055 | + | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| LR951_RS28450 (LR951_28390) | 21084..21239 | + | 156 | WP_172693602 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| LR951_RS28455 (LR951_28395) | 21240..21752 | - | 513 | WP_011091071 | hypothetical protein | traL |
| LR951_RS28460 (LR951_28400) | 21718..25065 | - | 3348 | WP_225577820 | LPD7 domain-containing protein | - |
| LR951_RS28465 (LR951_28405) | 25090..25350 | - | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| LR951_RS28470 (LR951_28410) | 25340..26503 | - | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| LR951_RS28475 (LR951_28415) | 26514..27293 | - | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| LR951_RS28480 (LR951_28420) | 27290..27790 | - | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| LR951_RS28485 (LR951_28425) | 27804..29783 | - | 1980 | WP_085844212 | TraI/MobA(P) family conjugative relaxase | - |
| LR951_RS28490 (LR951_28430) | 29770..30087 | - | 318 | WP_004206923 | plasmid mobilization protein MobA | - |
| LR951_RS28495 (LR951_28435) | 30333..30728 | + | 396 | WP_019725163 | hypothetical protein | - |
| LR951_RS28500 (LR951_28440) | 31229..31765 | - | 537 | WP_015058935 | hypothetical protein | - |
| LR951_RS28505 (LR951_28445) | 31898..32209 | - | 312 | WP_015058934 | hypothetical protein | - |
Host bacterium
| ID | 6680 | GenBank | NZ_CP089887 |
| Plasmid name | pKP102-3 | Incompatibility group | IncL/M |
| Plasmid size | 58472 bp | Coordinate of oriT [Strand] | 30091..30195 [-] |
| Host baterium | Klebsiella pneumoniae strain KP102 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |